Method for establishing three-dimensional tissue in vitro

Abstract

The invention provides a method for establishing a three-dimensional tissue in vitro. The method comprises the steps of separately culturing epithelial cells and muscle cells; implanting a small intestinal submucosa acellular matrix stent into a muscle cell suspension; continuing to culture after finishing oscillating and compounding operations; and dropwise adding an epithelial cell suspension on a muscle cell layer of the stent treated in the step 2, and continuing to culture to obtain the three-dimensional tissue. According to the method, the epithelial cells and the muscle cells of tongue mucosa are used as seed cells, so that materials are conveniently obtained, the cells can be increased to the relatively high quantity within relatively short time, and therefore, the application requirement is met; the muscle cells are compounded with the small intestinal submucosa acellular matrix stent by using an oscillating method, so that a plurality of muscle cells can be soaked into the stent and can be relatively uniformly distributed; meanwhile, the stent can be loosened as much as possible on the premise that the stent is not obviously damaged, so that nutrient permeation and neovascularization can be favorably realized; and the three-dimensional tissue which can be used for tissue engineering urethra can be established.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a method for constructing a three-dimensional tissue in vitro, and the three-dimensional tissue constructed by the method. Background technique [0002] Surgical repair of congenital or acquired urethral diseases is one of the difficult problems that are constantly being explored in the field of urology. The development of tissue engineering technology provides a new idea for the repair of urethral stricture. Among them, acellular matrix is ​​an important content of tissue engineering as a scaffold for cell regeneration. At present, the most studied acellular matrix is ​​small intestinal submucosal acellular matrix (SIS), which has been applied to clinical urethral repair and has achieved good results. [0003] The submucosal acellular matrix of the small intestine is a kind of allogeneic tissue obtained from the small intestine of pigs. The mucosal layer, serosa lay...

AI Technical Summary

Problems solved by technology

[0005] For the seed cells used in urethral tissue engineering, the most common traditional methods are urothelial cells and smooth muscle cells, but the acquisition of such seed cells often requires bladder biopsy or incision, which is inconvenient.

Images