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Small intestinal submucosa (SIS) soft tissue repair patch and preparation method thereof

A soft tissue and patch technology, which is applied in the preparation and preparation of SIS tissue repair materials, can solve the problems of degreasing and deproteinizing disinfection, low mechanical strength, single shape and structure of products, etc.

Inactive Publication Date: 2012-05-23
BEIJING MED ZENITH MEDICAL SCI CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the preparation process of SIS is simple, and most of them are limited to decellularization alone. Generally speaking, there will be problems of defat deproteinization or insufficient disinfection, which leads to certain immunogenicity and antigenicity of the material. At the same time, its single-layer product The mechanical strength is low, and the shape and structure of the product are single, which is not conducive to the repair of soft tissues

Method used

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  • Small intestinal submucosa (SIS) soft tissue repair patch and preparation method thereof
  • Small intestinal submucosa (SIS) soft tissue repair patch and preparation method thereof
  • Small intestinal submucosa (SIS) soft tissue repair patch and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0059] Take the fresh pig small intestine within 6 hours of slaughter, rinse the pig jejunum (between duodenum and ileum) with distilled water, cut into 20cm pieces, soak in 0.5% bromogeramine solution for 30min, and then rinse the rear edge with pure water. Cut open longitudinally, mechanically scrape off the small intestinal mucosa, muscle layer and serosal layer, soak and disinfect in a mixed aqueous solution containing 0.2% peracetic acid and 5% ethanol for 60 minutes, rinse with normal saline, and obtain the small intestinal submucosa; Then place it in disodium edetate (0.05M) solution for 2 hours and soak in a solution of sodium hydroxide (1%) (ph value is 11-12) for 8 hours at a temperature of 4°C ± 2°C. The submucosa of the small intestine was initially disinfected and decellularized; then soaked in a solution containing hydrochloric acid (1mol / L) for 4 hours, rinsed in phosphate buffered saline (PBS) until neutral; degreased with absolute ethanol Treat for 12 hours, t...

Embodiment 2

[0061] Adopt 3% pentobarbital sodium anesthesia intraperitoneal injection to anesthetize mice, then carry out relevant subcutaneous implantation test, method: first mouse back hair is cleaned up, then adopts puncher to respectively inject product of the present invention (embodiment 1) Implanted subcutaneously on both sides of the back of the mouse, then sutured the skin, and sterilized. After the operation, the animals were fasted for 12 hours, housed in separate cages, and penicillin was injected intramuscularly for three consecutive days. Observe the general state of the mice and the performance of the incision site. At the same time, the mice were sacrificed on day 4, 12, and 21, and the morphological changes and peripheral tissue growth of the SIS soft tissue repair patches were observed.

[0062] Results: The mice grew well after operation, and the surgical wound was basically healed after 4 days. The results of pathological sections showed that the SIS soft tissue repa...

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Abstract

The invention relates to a method for preparing a small intestinal submucosa (SIS) soft tissue repair patch, and the soft tissue repair patch prepared by the method. The invention also relates to application of the soft tissue repair path to tissue repair.

Description

technical field [0001] The present invention relates to a soft tissue repair patch and a preparation method thereof, in particular to a preparation of a SIS (small intestinal submucosa, small intestinal submucosa material) tissue repair material, a preparation method thereof and an application thereof. Background technique [0002] Tissue trauma or defect is a common clinical disease, such as trauma caused by internal surgery, partial defect of organ tissue, hernia, skin injury on the body surface, etc., which seriously affects the quality of life of the patient and is a problem that must be faced and solved urgently in medicine . [0003] At present, there are two main types of materials for tissue repair on the market: one is polymer synthetic materials such as polypropylene, polyester, and polytetrafluoroethylene, but because they are good adhesion carriers for bacteria, bacteria adhere to them. After being applied, it can produce a biofilm that protects it from the host...

Claims

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Application Information

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IPC IPC(8): A61F2/02A61L27/38
Inventor 孟坚丛敬徐小雨可大年
Owner BEIJING MED ZENITH MEDICAL SCI CORP LTD
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