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Tissue repair material, preparation method thereof and application

A tissue repair and raw material technology, applied in medical science, prosthesis, etc., to achieve the effect of prolonging the action time and enhancing the proliferation activity

Active Publication Date: 2012-10-24
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the combination of SIS and NGF to promote wound healing

Method used

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  • Tissue repair material, preparation method thereof and application
  • Tissue repair material, preparation method thereof and application
  • Tissue repair material, preparation method thereof and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Preparation of the tissue repair material of the present invention

[0043] The small intestinal submucosa (SIS) was mixed with 0.5M acetic acid at 2:100W / V, stirred intermittently by a tissue homogenizer at 4°C for 1h until gelatinous, and placed in a self-made cylindrical mold. Then add NGF to 100ng / ml to the gel-like SIS and stir evenly. After overnight pre-freezing at -20°C, freeze-drying and ethylene oxide sterilization, the tissue repair material of the present invention (NGF-SIS) was obtained. It has been determined that the content of NGF in the tissue repair material of the present invention is 5ng / mg; when in use, take an appropriate amount of NGF-SIS and add water to swell it, and apply it to the wound.

[0044] The small intestinal submucosa described in the present invention is the small intestinal submucosa acellular matrix. At present, the main preparation method is to take the empty small intestine, wash it with water, remove the serosa layer,...

Embodiment 2

[0050] The present invention can also adopt the reported method to prepare SIS, for example, Luo Jingcong, et al., Research on Cytocompatibility of Small Intestine Submucosa, Journal of Biomedical Engineering, Volume 21, Issue 5, 2004; or, application number: CN200710177285. 6. Invention title: A preparation method of SIS tissue repair material. Example 2 The preferred concentration of NGF in the tissue repair material of the present invention

[0051] 1. Main reagents and instruments

[0052] 1.1 Reagents

[0053] Recombinant human β-NGF (Peprotech, U.S.); β-NGF antibody (abcam, U.K.); MTT (Sigma, U.S.); DMEM / F12 medium (GIBCO, U.S.); Industrial Bioengineering Technology Service Co., Ltd.);

[0054] 1.2 Instruments

[0055] Inverted phase contrast microscope (Olympus, Japan); fluorescence microscope (Olympus, Japan); continuous spectrum microplate optical density reader (Molecular Devices, USA); real-time fluorescent quantitative gene amplification instrument (Bio-Rad, US...

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Abstract

The invention discloses a tissue repair material which comprises an SIS (small intestinal submucosa) and an NGF (nerve growth factor). When the NGF is individually used or simply mixed with the SIS in use, the NGF is rapidly inactivated and is unfavorable for wound repair. However, after the composite material is prepared with the NGF and the SIS according to the specific method, the action time of the NGF can be effectively prolonged, the cell proliferation activity of the NGF-SIS composite material is remarkably enhanced as compared with that of the individually used SIS, and the NGF-SIS composite material plays a role in synergy.

Description

technical field [0001] The invention relates to a tissue repair material and its preparation method and application, belonging to the field of biological materials. Background technique [0002] Wound is the damage of normal skin or tissue caused by external injury factors and internal factors of the body. Often accompanied by damage to the integrity of the skin and loss of a certain amount of normal tissue, at the same time, the normal function of the skin is also impaired. Wounds are mainly divided into acute wounds and chronic wounds. Common acute wounds include surgical incisions, skin abrasions, burns, etc.; common chronic wounds include decubitus ulcers, vascular ulcers of lower extremities, diabetic foot ulcers, and other difficult-to-heal wounds. Once the wound surface is formed, the body will respond quickly and start the healing process to repair it. Ordinary acute wounds can be healed through the body's own repair, while most chronic wounds, especially difficul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/54
Inventor 罗静聪钱志勇解慧琪杨志明
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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