Preparation method of paper-based ratio photoelectrochemical biosensor for detecting microRNA

A biosensor, photoelectrochemical technology, applied in the direction of biochemical equipment and methods, electrochemical variables of materials, measurement/inspection of microorganisms, etc., can solve the problem of low microRNA content, improve signal amplification ability, reduce internal and external interference, The effect of accelerating the separation and transfer of photogenerated charges

Inactive Publication Date: 2020-04-17
UNIV OF JINAN
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AI Technical Summary

Problems solved by technology

However, the content of microRNA is low, in order to achieve quantitative detection of microRNA, it is necessary to improve the accuracy and sensitivity of the analysis method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Application of paper-based ratio photoelectrochemical biosensor in detecting microRNA-141

[0021] (1) First, use Adobe illustrator CS4 software to design the batik patterns of the paper auxiliary board and the paper detection board, and then print the designed batik patterns on the chromatogram paper through the wax printer model Color Qube 8580, and finally print the wax The chromatographic paper is heated in an oven at 130 ℃ for 50 s to obtain the hydrophilic auxiliary area of ​​the paper auxiliary board, the hydrophilic working electrode area and the internal reference electrode area of ​​the paper detection board;

[0022] (2) The hydrophilic working electrode area and the internal reference electrode area of ​​the paper test board obtained in step (1) are functionalized gold nanoparticle conductive layers. The specific steps are as follows: first prepare gold seeds, and add 100 μL of 0.24 M Mercaptoethylamine was added to 10 mL of 1.5 mM chloroauric acid sol...

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Abstract

The invention discloses a preparation method of a paper-based ratio photoelectrochemical biosensor for detecting microRNA. Gold nanoparticles grow on the surfaces of paper-based dual electrodes composed of a working electrode and an internal reference electrode, then cuprous oxide is electrodeposited, graphene quantum dots and silver iodide nanoparticles are sensitized, and a photocurrent signal is enhanced; when the target microRNA exists, DNA probes output by microRNA-induced double-stranded specific nuclease reactions with different concentrations and constant concentrations are respectively incubated on the surfaces of the working electrode and the internal reference electrode; DNA bridge chains and DNA hairpins H1 and H2 on the surface of the electrode are combined to form a DNA bridge nano structure through induction; and the silver iodide nanoparticles marked at the end parts of the H1 and the H2 are far away from the surface of the electrode, so that the photocurrent signal isreduced, and the sensitive detection of the microRNA is realized based on the ratio of the working current signal to the internal reference current signal.

Description

Technical field [0001] The invention relates to the field of photoelectrochemical signal detection technology, paper-based sensor device preparation technology, nanocomposite material synthesis technology and microRNA analysis and detection technology, and more specifically to the preparation of a paper-based ratio photoelectrochemical biosensor for detecting microRNA method. Background technique [0002] In recent years, based on the comprehensive advantages of microfluidic paper analysis devices and photoelectrochemical analysis methods, such as low cost, portability, simple operation, and high sensitivity, various paper-based photoelectrochemical analysis devices have been prepared. In the process of paper-based photoelectrochemical analysis, the single-signal readout mode is usually used. This single-signal analysis strategy is susceptible to interference from the external environment, analysis equipment, target analytes, etc., resulting in false positive / negative signals and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/38G01N27/327C12Q1/6825
CPCC12Q1/6825G01N27/301G01N27/305G01N27/308G01N27/3272G01N27/3273G01N27/3278G01N27/38
Inventor 于京华杨红梅李正林胡孟苏张彦张丽娜葛慎光
Owner UNIV OF JINAN
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