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A h-bn/mos for targeted photothermal and chemical synergistic therapy 2 Nanoprobe and its preparation method and application

A nanoprobe, h-bn technology, applied in the field of nanoprobes, can solve the problems of limited sensitivity, single treatment method, no fluorescence and Raman dual-mode detection and analysis, etc. The effect of high infrared absorption and superior optical properties

Active Publication Date: 2022-08-02
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0007] (1) In the process of cell detection, h-BN nanosheets are only used for Raman signal detection, with a single detection standard, high background signal and limited sensitivity;
[0008] (2) Existing probes are only for fluorescence or Raman monitoring, but no fluorescence and Raman dual-mode detection analysis;
[0009] (3) The existing technology uses h-BN as a carrier to adsorb a single therapeutic drug, and performs chemotherapy and photodynamic therapy separately, with a single treatment method and limited therapeutic effect;
[0010] (4) At the present stage, when using probes for detection and treatment, the method is single, and Raman and fluorescence dual-modal detection cannot be realized, as well as the coordinated treatment of chemotherapy and photothermal therapy, which has certain advantages in the construction of an integrated platform for diagnosis and treatment. limitation

Method used

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  • A h-bn/mos for targeted photothermal and chemical synergistic therapy <sub>2</sub> Nanoprobe and its preparation method and application
  • A h-bn/mos for targeted photothermal and chemical synergistic therapy <sub>2</sub> Nanoprobe and its preparation method and application
  • A h-bn/mos for targeted photothermal and chemical synergistic therapy <sub>2</sub> Nanoprobe and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] h-BN / MoS 2 The nanoprobe is prepared according to the following preparation method:

[0059] (1) Synthesis of h-BN nanosheets

[0060] Dissolve 1g of hexagonal boron nitride powder in 50mL of concentrated sulfuric acid, and use magnetic stirring to slowly add 0.5g of KMnO 4 , the solution changed from white to green, and continued stirring for 12h. Then, 10 mL of 30% H was added to the mixed solution 2 O 2 . After the solution was cooled to room temperature, the suspension was obtained by ultrasonic treatment for 1 h. The suspension was centrifuged for 10 min, and the centrifuged white solid powder at 8000-12000 rpm was retained to remove the h-BN nanosheets with too large or too small particle size. The centrifuged white solid powder was washed repeatedly with deionized water until the pH of the solution was neutral. Finally, the solid powder was vacuum-dried at 60 °C for 12 h to obtain h-BN nanosheets.

[0061] (2) MoS 2 Synthesis and modification of quantum d...

Embodiment 2

[0094] Example 2 DOX@h-BN / MoS 2 Construction and application of nanoformulations

[0095] ①DOX loading and releasing

[0096] The h-BN / MoS 2 Nanoprobes (0.2mL, 1.0mg / mL) and DOX solution (0.2mL, 1.0mg / mL) were diluted to 1mL with PBS buffer, shaken at room temperature for 24h, and centrifuged to separate DOX@h-BN / MoS 2 Nanoformulations and unloaded DOX solutions. The supernatant solution was collected, using a Hitachi F-4600 fluorescence spectrophotometer (E X = 488 nm) to measure the fluorescence of the supernatant. And compared h-BN / MoS by hydrodynamic particle size and Zeta potential experiments 2 Changes before and after loading the nanoprobe with DOX and PBS buffers with different pH (pH=5.5, 6.5 and 7.4), and the DOX fluorescence after shaking at room temperature for 12 h.

[0097] Further investigation of DOX@h-BN / MoS by fluorescence in the supernatant under different pH buffer media 2 Release capacity of nanoformulations. The DOX@h-BN / MoS 2 Nanoformulations (2...

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Abstract

The invention relates to the technical field of nanoprobes, in particular to a h-BN / MoS capable of realizing targeted photothermal and chemical synergistic therapy 2 Nanoprobe and preparation method and application thereof, the preparation method includes: h-BN nanosheet dispersion and FA-PEI modified MoS 2 Equal volume mixing of quantum dot dispersion to obtain h‑BN / MoS 2 Composite materials; preparation of DNA hairpins, h‑BN / MoS 2 The composites adsorb DNA strands to obtain h‑BN / MoS 2 Nanoprobe; h‑BN / MoS 2 Nanoprobes can be used for miRNA fluorescence and Raman dual-mode detection and / or fluorescence in situ imaging and preparation of nanoformulations. The invention broadens the application of 2D nanosheets, realizes photothermal and chemical therapy, and also lays a foundation for further developing a diagnosis and treatment integrated platform for cancer marker detection and synergistic therapy.

Description

technical field [0001] The invention relates to the technical field of nanoprobes, in particular to an h-BN / MoS capable of realizing targeted photothermal and chemical synergistic therapy 2 Nanoprobe and preparation method and application thereof. Background technique [0002] 2D nanocomposites are nanomaterials for meaningful biomedical applications due to their excellent physicochemical properties and high diversity in morphology, size, and biodegradability, and are widely used in multimodal state-of-the-art imaging, biosensors, drug / gene delivery, and cancer therapy. [0003] Due to their large specific surface area, 2D composite nanomaterials can effectively adsorb various types of molecules, such as drugs, fluorescent probes, nucleic acids, proteins, and other molecules, through covalent or non-covalent interactions, and stimulate them through targeted stimulation. , to achieve a controlled release. Including Fe 3 O 4 Nanoparticles, TiO 2 A series of functional na...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00
CPCA61K49/0002A61K47/6923A61K31/704A61K47/545A61K41/0052A61P35/00C09K11/681C09K11/63C09K11/0883B82Y5/00B82Y20/00B82Y30/00B82Y40/00A61K49/0021A61K49/0013A61K49/005A61K49/0067A61K49/0054A61K2300/00
Inventor 何鹏李甜甜隋永鹍赵林韩文豪李东祥徐景坤毕成
Owner QINGDAO UNIV OF SCI & TECH
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