34 results about "Biocomputer" patented technology

By means of biological computer technology, one group of gene sequence relevant to human tumor is obtained from GenBank. Based on RNA interference technology, one group of siRNA capable of inducing RNA interference is designed. Certain amount of siRNA is synthesized chemically and used as novel efficient and specific antitumor medicine. MTT detection shows that siRNA has obvious cytotoxic effect on MCF-7, BEL 7402, KB, HT-29 and other tuomr cells. Western Blotting detection shows that siRNA can lower the expression level of specific protein by 80-90%. Morphological observation shows that siRNA can cause tumor cell produce corresponding morphological change and chromatin concentration. Compared with available technology, siRNA is one antitumor medicine with completely new mechanism, high efficiency and fast speed.

The present disclosure provides a bacterial cell computing component that indicates priority of a signal at different accompanying signals. A cell calculation part is composed of a first cell and a second cell. The first cell is used for detecting a signal to be detected and a first accompanying signal, and the second cell is used for detecting the signal to be detected and a second accompanying signal; the gene lines in the first cell comprise a first line and a second line, and the gene lines in the second cell comprise a third line and a fourth line. According to the cell calculation part provided by the invention, by designing a gene line, activation of the promoter and inhibition of the indication signal are used for making different indications on no signal to be detected, only the signal to be detected, the signal to be detected and the corresponding accompanying signal exist at the same time, and a final result is indicated through output combination of multiple cells. The component can be used for related applications such as a biological computer and a biosensor which need to judge the priority of a certain specific signal in different states, and has the advantages of calculation accuracy, high efficiency and usability.

The invention provides an implementation method of a two-bit Gray code subtractor molecular circuit based on DNA strand displacement. The implementation method is used for solving the problem of two-bit Gray code subtractor molecular operation based on Gray codes. The method comprises the following steps of: listing an operation truth table according to an operation rule of a two-bit Gray code subtractor circuit; converting the two-bit Gray code subtractor circuit into a two-bit Gray code subtractor double-track logic circuit by using a double-track idea; researching a DNA strand displacementreaction principle based on DNA strand displacement reaction; designing and cascading all DNA molecule fan-out doors, DNA molecule AND gates, DNA molecule OR gates and DNA report gate structures according to a DNA strand displacement reaction principle, and constructing a two-bit Gray code subtractor molecular circuit; visual DSD software is used for carrying out simulation analysis on the molecular circuit, and the dynamic characteristics of the molecular circuit are verified. According to the invention, a basic theoretical basis is provided for constructing a more complex logic operation circuit in the future, and the development of the biological computer is promoted, so that the reliability of the logic circuit of the biological computer is improved.

The invention relates to the field of biological computing protein structure prediction, and provides a hybrid search optimizing method based on a BSA (bovine serum albumin) algorithm and a TS (tabu search) algorithm. According to the method, the BSA algorithm is applied to the protein three-dimensional structure prediction, and the BSA algorithm and the TS algorithm are respectively improved to a certain degree. The experiment is performed on a real protein sequence and a Fibonacci sequence which are widely used in the prior art; by analyzing the comparison result of data obtained from the experiment and other methods, the method better overcomes the defect that the conventional algorithm is more sensitive to the initial value of control parameters and can be easily caught in the local optimization; high performance and high precision are realized.

The invention relates to a DNA network construction method for regulating the function of E6 ribozyme based on strand displacement. Based on the hairpin-like secondary structure of the E6 ribozyme and the sequence of the catalytic core conservative domain, the method realizes the regulation of the function of the E6 ribozyme through strand displacement technology; the substrate binding arm of the E6 ribozyme is used to add an RNA-modified DNA substrate, Form a branch ring structure to construct a DNA logic computing unit; construct a DNA logic gate by transforming the DNA logic computing unit; finally connect the DNA logic gate to form a DNA network. The method does not destroy the sequence integrity of the E6 ribozyme, and the DNA logic computing unit has the characteristics of stable structure, customization, modularization, low leakage, and anti-interference, and can be widely used in biological computing and DNA nanostructures.

The present invention proposes a four-input factorial addition molecular circuit design method based on DNA chain replacement, the steps of which are: write out the factorials corresponding to two binary numbers, and list ten kinds of factorial sums of two binary numbers according to random combinations The truth table of the factorial addition operation circuit is constructed; the four-input factorial addition operation circuit is transformed into a dual-track logic circuit containing only logical AND gates and logical OR gates by adopting the dual-track idea, and DNA molecular logic gates are designed by using DNA molecules and four-input factorial is constructed. For the molecular circuit of addition operation, the correctness of the output results is verified by Visual DSD simulation software, the complex dynamic behavior of the four-input factorial addition operation molecular circuit is analyzed, and its dynamic behavior is verified. The invention provides a basic theoretical basis for constructing more complex logical operation circuits in the future, promotes the development of biological computers, and thus improves the reliability of biological computer logic circuits.

The invention discloses a multi-cell signal size comparator, a comparison method and an application. The comparator includes four kinds of bacterial cells, the first cell and the second cell are input receiving cells, and the third cell and the fourth cell are result output cells; The first cell corresponds to the first input bit, the second cell corresponds to the second input bit, the third cell corresponds to the first output bit, and the fourth cell corresponds to the second output bit; between the first cell and the third cell The first chemical line or the second chemical line communicates, the second cell and the third cell communicate through the second chemical line, the first cell and the fourth cell communicate through the second chemical line, the second cell and the fourth cell They communicate with each other through the second chemical line or the third chemical line. Through multi-cell cooperation, the size judgment of the input signals of two input bits can be judged, and whether the two input bit signals are equal can be used in related applications such as biological computers that require size comparison.

The invention publishes a kind of especial siRNA and expression plasmid, which can control men's siRNA gene expression, and their application in curing or preventing knub. The invention can get a group of bcl-2 gene orders from geneBank by biotechnology and bases on the RNA interference technology. By this condition, a group of siRNA which can induce RNA interference and can be gotten. And quantitative siRNA by chemical composition can be composed to control bcl-2 gene expression and to control neoplasitic growth. The siRNA and the expression plasmid can be used to produce antineoplasitic that has high active, high specificity and little side effect.

The invention publish a kind of siRNA and express carrier, which can control men's VEGF gene expression, and their application in preparing drug for affection relation to VEGF gene. The invention get a group VEGF orders by biocomputer technology and work out a group of siRNA to induce RNA disturbance basing on the RNA disturbance technology. After this, compose.

The present invention relates to an AND gate molecular circuit, a NOT gate molecular circuit, an XOR molecular circuit and a half-subtracter molecular circuit based on a DNA hairpin structure, belonging to the biocomputer technology field. The principles of the DNA single hairpin structure, the double hairpin structure and the two-way strand displacement reaction are employed to design a strand displacement reaction structure taking a conventional small fulcrum as an identification area to realize the construction of AND gate, NOT gate and XOR gate basic logic operation structures and perform globality simulation argumentation. A basic logic circuit is employed to perform combination design of the half-subtracter molecular circuit and perform argumentation simulation of the circuit feasibility.

The invention relates to the technical fields of biological computing and Monte Carlo simulation, and particularly discloses a Monte Carlo simulation-based protein thermomechanical-analysis method, which comprises the following steps of A, determining a protein energy model; B, simulating and calculating the state density of a protein system. According to the method, the whole thermomechanical process of protein folding can be efficiently analyzed and researched to further explore and research a protein folding process.

The invention provides a bacterial cell 3-8 decoder and a cell computer. 8 cells from a first cell to an eighten cell are composed of a first characteristic signal promoter, a second characteristic signal promoter, a third characteristic signal promoter, a constitutive promoter, a promoter inhibition element, a promoter deinhibition element, an indication signal inhibition element and an indication signal deinhibition element. The decoder is a multi-cell computing component, decoding calculation is completed through activation and inhibition operation on the promoter and the indication signal,and the decoder has the accuracy, flexibility and usability of calculation. The bacterial cell 3-8 decoder can be used in related applications requiring a decoder such as a bio-computer, a biosensorand the like and has a wide range of applications.