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A kind of multi-cell signal size comparator, comparison method and application

A technology of signal size and comparator, applied in the field of cellular computer, which can solve the problems of errors, inability to judge the same signal, and inability to make equal judgments.

Active Publication Date: 2021-02-09
MINZU UNIVERSITY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] (1) Only one cell accepts input, so only different signals can be judged, and the same signal cannot be judged (that is, equal judgment cannot be made)
If the biological computer needs to compare the results of two sub-tasks to determine the next step during operation, and the results of the two tasks are equal in size (a signal), the above-mentioned calculation components can only use it as an input , resulting in an error;
[0005] (2) The user needs to know the type of the signal to be compared before the calculation. If the biological computer needs to compare the results of the two subtasks to determine the next step during the operation, the user needs to first identify the substance type of the result Then compare and calculate, adding an intermediate step

Method used

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  • A kind of multi-cell signal size comparator, comparison method and application
  • A kind of multi-cell signal size comparator, comparison method and application
  • A kind of multi-cell signal size comparator, comparison method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] This example is used to compare the size of the first signal IPTG (isopropyl-β-D-thiogalactopyranoside) and the second signal Arabinose (arabinose); green fluorescence is used as the indicator signal; DNA fixed point is used The flipping function of the recombinase Cre / loxP system realizes the uninhibition / inhibition of the indication signal, thereby realizing the uninhibition / inhibition of the circuit.

[0068] Promoters, chemical lines and signature signals are selected as follows:

[0069] The first signal promoter: the promoter P initiated by the first signal IPTG lac .

[0070] The second signal promoter: the promoter P initiated by the second signal Arabinose BAD .

[0071] Indicator signal: green fluorescent protein GFP.

[0072] The first chemical line: quorum sensing signal molecule 3OC12-HSL, the corresponding gene is LasI, and the corresponding promoter is P las .

[0073] The second chemical line: quorum sensing signal molecule C4-HSL, the corresponding ...

example 1

[0075] The gene circuit diagram of each cell in Example 1 is as follows Figure 6-9 shown.

[0076] In each input case, the calculation process of Example 1 is:

[0077] (1) The first input bit: IPTG, the second input bit: Arabinose

[0078] The first cell works in the 1-1 line expressing the first chemical line 3OC12-HSL; the second cell works in the 2-2 line expressing the second chemical line C4-HSL; the third cell expresses the second chemical in the 3-2 line line promoter P rhl Start the expression of Cre recombinase, the GFP in the 3-1 line is turned forward, under the action of the first chemical line 3OC12-HSL, the first chemical line promoter P las Initiate the expression of the indicator signal GFP, the first output is detectable green fluorescence; the second chemical line promoter P in the 4-2 line in the fourth cell rhl Initiate the expression of Cre recombinase, the GFP in the 4-1 line is flipped forward, but because there is no third chemical line AHL, the t...

Embodiment 2

[0106] Just replace the Cre / loxP system in Example 1 with the FLP / frt system. Correspondingly, the gene circuit diagram of each cell is shown in 10-13. Promoters, chemical lines and characteristic signals, etc. and results are also shown in Example 1.

[0107] The beneficial effect of the present invention is: through the combination of three different intermediate signals (the first chemical line, the second chemical line, the third chemical line) instead of various combinations of two-bit input to realize the size of the two input bit input signals Comparison, the principle is: transform the smaller signal into the second chemical line, and convert the larger signal into the first chemical line (first input bit) and the third chemical line (second input bit) according to the difference of its input bit. ). For each output bit cell, the indicator signal can only be expressed under the action of the intermediate signal of the two signals, that is, the first chemical line and...

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Abstract

The invention discloses a multi-cell signal size comparator, a comparison method and an application. The comparator includes four kinds of bacterial cells, the first cell and the second cell are input receiving cells, and the third cell and the fourth cell are result output cells; The first cell corresponds to the first input bit, the second cell corresponds to the second input bit, the third cell corresponds to the first output bit, and the fourth cell corresponds to the second output bit; between the first cell and the third cell The first chemical line or the second chemical line communicates, the second cell and the third cell communicate through the second chemical line, the first cell and the fourth cell communicate through the second chemical line, the second cell and the fourth cell They communicate with each other through the second chemical line or the third chemical line. Through multi-cell cooperation, the size judgment of the input signals of two input bits can be judged, and whether the two input bit signals are equal can be used in related applications such as biological computers that require size comparison.

Description

technical field [0001] The invention relates to the technical field of cell computers, in particular to a multi-cell signal size comparator, comparison method and application. Background technique [0002] The construction of cellular computing components is one of the current hot issues. At present, various logic gates such as cellular AND, OR, and NOT, as well as cellular computing components such as bistable switches, memories, and counters, have been successfully constructed. A comparator is a basic computing component that compares two or more data items to determine whether they are equal, or to determine the magnitude relationship between them. A circuit or device capable of performing this comparison function is called a comparator. [0003] The current cell comparator such as "Construction of a genetic comparator in Escherichia Coli" (Chen Mei, IEEE Access, vol.7, pp.85024-85028, Jun, 2019) published by the inventor in the paper, and as the inventor previously Pa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/21G01N21/64C12R1/19
CPCC12N1/205C12R2001/19G01N21/6428
Inventor 陈梅程小艳施娜韦洁瑶
Owner MINZU UNIVERSITY OF CHINA
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