A kind of multi-cell signal size comparator, comparison method and application
A technology of signal size and comparator, applied in the field of cellular computer, which can solve the problems of errors, inability to judge the same signal, and inability to make equal judgments.
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Embodiment 1
[0067] This example is used to compare the size of the first signal IPTG (isopropyl-β-D-thiogalactopyranoside) and the second signal Arabinose (arabinose); green fluorescence is used as the indicator signal; DNA fixed point is used The flipping function of the recombinase Cre / loxP system realizes the uninhibition / inhibition of the indication signal, thereby realizing the uninhibition / inhibition of the circuit.
[0068] Promoters, chemical lines and signature signals are selected as follows:
[0069] The first signal promoter: the promoter P initiated by the first signal IPTG lac .
[0070] The second signal promoter: the promoter P initiated by the second signal Arabinose BAD .
[0071] Indicator signal: green fluorescent protein GFP.
[0072] The first chemical line: quorum sensing signal molecule 3OC12-HSL, the corresponding gene is LasI, and the corresponding promoter is P las .
[0073] The second chemical line: quorum sensing signal molecule C4-HSL, the corresponding ...
example 1
[0075] The gene circuit diagram of each cell in Example 1 is as follows Figure 6-9 shown.
[0076] In each input case, the calculation process of Example 1 is:
[0077] (1) The first input bit: IPTG, the second input bit: Arabinose
[0078] The first cell works in the 1-1 line expressing the first chemical line 3OC12-HSL; the second cell works in the 2-2 line expressing the second chemical line C4-HSL; the third cell expresses the second chemical in the 3-2 line line promoter P rhl Start the expression of Cre recombinase, the GFP in the 3-1 line is turned forward, under the action of the first chemical line 3OC12-HSL, the first chemical line promoter P las Initiate the expression of the indicator signal GFP, the first output is detectable green fluorescence; the second chemical line promoter P in the 4-2 line in the fourth cell rhl Initiate the expression of Cre recombinase, the GFP in the 4-1 line is flipped forward, but because there is no third chemical line AHL, the t...
Embodiment 2
[0106] Just replace the Cre / loxP system in Example 1 with the FLP / frt system. Correspondingly, the gene circuit diagram of each cell is shown in 10-13. Promoters, chemical lines and characteristic signals, etc. and results are also shown in Example 1.
[0107] The beneficial effect of the present invention is: through the combination of three different intermediate signals (the first chemical line, the second chemical line, the third chemical line) instead of various combinations of two-bit input to realize the size of the two input bit input signals Comparison, the principle is: transform the smaller signal into the second chemical line, and convert the larger signal into the first chemical line (first input bit) and the third chemical line (second input bit) according to the difference of its input bit. ). For each output bit cell, the indicator signal can only be expressed under the action of the intermediate signal of the two signals, that is, the first chemical line and...
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