Short interference ribonucleic acid as novel anti-tumor gene therapeutic medicine

A deoxyribonucleic acid, anti-tumor technology, applied in the field of a group of novel anti-tumor gene therapy drugs short-interfering RNA, can solve the problems of decreased activity and difficulty in judging efficacy
CN1425466AInactive Publication Date: 2003-06-25MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
Publication Date
2003-06-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

By means of biological computer technology, one group of gene sequence relevant to human tumor is obtained from GenBank. Based on RNA interference technology, one group of siRNA capable of inducing RNA interference is designed. Certain amount of siRNA is synthesized chemically and used as novel efficient and specific antitumor medicine. MTT detection shows that siRNA has obvious cytotoxic effect on MCF-7, BEL 7402, KB, HT-29 and other tuomr cells. Western Blotting detection shows that siRNA can lower the expression level of specific protein by 80-90%. Morphological observation shows that siRNA can cause tumor cell produce corresponding morphological change and chromatin concentration. Compared with available technology, siRNA is one antitumor medicine with completely new mechanism, high efficiency and fast speed.
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Description

Technical field:

[0001] The present invention relates to the design and synthesis of a group of siRNA (short interfering ribonucleic acid) drugs and their application in tumor gene therapy. Background technique:

[0002] Inhibiting the expression of tumorigenesis-related genes is an important idea in the research and development of new anti-tumor drugs. The RNA interference (RNAi) technology (Nature 1998; 391: 806-811), discovered in 1998, has become an effective tool to inhibit the expression of target genes. RNA interference is considered to be a physiological mechanism to defend against exogenous nucleic acid intrusion, which is common in lower organisms to higher organisms, and it can rapidly and specifically degrade target mRNA. Therefore, based on the RNA interference that exists in this organism itself, artificially design and synthesize specific siRNA targeting tumor-related genes as a precursor to induce RNA interference, so that it can effectively degrade tumor-re...

Claims

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