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Bacterial cell 3-8 decoder and cell computer

A technology of bacterial cells and decoders, applied in the field of synthetic biology, can solve the problem that there is no decoder yet

Active Publication Date: 2019-10-08
MINZU UNIVERSITY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there are no decoders built in cells

Method used

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  • Bacterial cell 3-8 decoder and cell computer
  • Bacterial cell 3-8 decoder and cell computer
  • Bacterial cell 3-8 decoder and cell computer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] This embodiment is used to decode the input conditions of IPTG (first signal), aTc (second signal), and Arabinose (third signal); green fluorescence is used as an indicator signal; CRISPR / dCas9 system is used to realize the indicator Signal inhibition, dCas9 protein is a protein that loses Cas9 cutting activity, cannot cut DNA, but can bind to DNA to prevent transcription, use sgRNA to guide dCas9 protein to bind to the corresponding DNA fragment, thereby preventing transcription from occurring and making gene elements Unable to express; using the flipping function of the DNA site-directed recombinase Cre / loxP system to realize promoter de-repression / repression; using the flipping function of the DNA site-directed recombinase FLP / frt system to realize indication signal de-repression / repression.

[0057] Promoters and signature signals are selected as follows:

[0058] The first promoter: the promoter P activated by the first signal IPTG lac

[0059] Second promoter: p...

Embodiment 2

[0083] In this example, the CRISPR / dCas9 system in Example 1 was replaced with CRISPR / ddCpf1 to achieve the effect of inhibiting gene expression. The ddCpf1 protein is similar to the dCas9 protein in that it cannot cut DNA, but it can bind to DNA to prevent the occurrence of transcription. The sgRNA is used to guide the ddCpf1 protein to bind to the corresponding DNA fragment, thereby preventing the occurrence of transcription and preventing the expression of genetic elements. In this example, the sgRNA is designed according to the requirements of the CRISPR / ddCpf1 system, and E. coli strains or other strains that can ensure the work of CRISPR / ddCpf1 should be selected or constructed. Others are the same as in Example 1.

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Abstract

The invention provides a bacterial cell 3-8 decoder and a cell computer. 8 cells from a first cell to an eighten cell are composed of a first characteristic signal promoter, a second characteristic signal promoter, a third characteristic signal promoter, a constitutive promoter, a promoter inhibition element, a promoter deinhibition element, an indication signal inhibition element and an indication signal deinhibition element. The decoder is a multi-cell computing component, decoding calculation is completed through activation and inhibition operation on the promoter and the indication signal,and the decoder has the accuracy, flexibility and usability of calculation. The bacterial cell 3-8 decoder can be used in related applications requiring a decoder such as a bio-computer, a biosensorand the like and has a wide range of applications.

Description

technical field [0001] The present disclosure relates to the technical field of synthetic biology, in particular, to a bacterial cell 3-8 decoder and a cell computer. Background technique [0002] The construction of cellular computing components is one of the current hot issues. At present, various logic gates such as cellular AND, OR, and NOT, as well as cellular computing components such as bistable switches, memories, and counters, have been successfully constructed. [0003] A decoder is a logic circuit with a "translation" function, and its function is to distinguish codes with specific meanings and convert them into control signals. If there are n binary input lines, it can be decoded and converted into 2 n output data. The decoder has a wide range of uses in the world, and can be used for code conversion, terminal digital display, data distribution, memory addressing and combination control signals, etc. It is a commonly used computing component. [0004] The inp...

Claims

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Application Information

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IPC IPC(8): G06N3/00
CPCG06N3/002
Inventor 陈梅赵洪帅
Owner MINZU UNIVERSITY OF CHINA
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