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Bacterial cell computing component that indicates priority of a signal at different accompanying signals

A technology for indicating signals and computing components, which is applied in the field of synthetic biology and can solve problems such as incomparability

Active Publication Date: 2019-09-27
MINZU UNIVERSITY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing cell size comparator can only compare the size of three different substances, but cannot compare the size of the same substance in different states, thereby indicating its priority

Method used

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  • Bacterial cell computing component that indicates priority of a signal at different accompanying signals
  • Bacterial cell computing component that indicates priority of a signal at different accompanying signals
  • Bacterial cell computing component that indicates priority of a signal at different accompanying signals

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The signal S to be detected, the first accompanying signal S1 , the second accompanying signal S2 , the first indication signal R1 , and the second indication signal R2 are shown in the table below.

[0045] Signal to be detected S Ara (arabinose) First accompanying signal S1 IPTG (isopropyl-β-D-thiogalactopyranoside) Second accompanying signal S2 aTc (Anhydrotetracycline) The first indication signal R1 Green fluorescent protein GFP (that is, green light means 0) The second indication signal R2 Red fluorescent protein RFP (that is, red light indicates 1)

[0046] The promoter is specifically: the S promoter adopts the promoter P initiated by Ara BAD , the S1 promoter adopts the promoter P initiated by IPTG lac , the S2 promoter adopts the promoter P initiated by aTc tet .

[0047] In this example, the CRISPR / dCas9 system is used to realize the indicator signal inhibition function. The dCas9 protein is a protein that loses th...

Embodiment 2

[0056] In this example, the CRISPR / dCas9 system in Example 1 is replaced with CRISPR / ddCpf1. The ddCpf1 protein is similar to the dCas9 protein and cannot cut DNA, but it can bind to DNA to prevent transcription from occurring. sgRNA is used to guide the ddCpf1 protein and the corresponding DNA fragment Binding, thereby preventing the occurrence of transcription and making the gene element unable to be expressed. E. coli strains or other strains that can ensure the work of CRISPR / ddCpf1 should be selected or constructed, and the sgRNA design can be in accordance with the requirements of the CRISPR / ddCpf1 system, and the others are the same as in Example 1.

Embodiment 3

[0058] In this embodiment, the Cre recombinase and loxP sites in Example 1 are replaced with FLP recombinase and frt sites, such as image 3 , and others are the same as in Embodiment 1. The function of FLP recombinase is similar to that of Cre recombinase, and the frt site is its corresponding site. FLP recombinase can delete the fragment between two forward frt sites, and can also flip between two reverse frt sites. fragments.

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Abstract

The present disclosure provides a bacterial cell computing component that indicates priority of a signal at different accompanying signals. A cell calculation part is composed of a first cell and a second cell. The first cell is used for detecting a signal to be detected and a first accompanying signal, and the second cell is used for detecting the signal to be detected and a second accompanying signal; the gene lines in the first cell comprise a first line and a second line, and the gene lines in the second cell comprise a third line and a fourth line. According to the cell calculation part provided by the invention, by designing a gene line, activation of the promoter and inhibition of the indication signal are used for making different indications on no signal to be detected, only the signal to be detected, the signal to be detected and the corresponding accompanying signal exist at the same time, and a final result is indicated through output combination of multiple cells. The component can be used for related applications such as a biological computer and a biosensor which need to judge the priority of a certain specific signal in different states, and has the advantages of calculation accuracy, high efficiency and usability.

Description

technical field [0001] The present disclosure relates to the technical field of synthetic biology, in particular, to a bacterial cell computing component that indicates the priority of signals under different accompanying signals. Background technique [0002] The construction of cellular computing components is one of the hot issues in synthetic biology. At present, various logic gates such as cellular AND, OR, and NOT, as well as cellular computing components such as bistable switches, oscillators, and memories, have been successfully constructed. [0003] Priority is a parameter that determines the priority level of each task to receive system resources when the computer system processes multiple computing tasks. The cellular computer is limited by the computing resources in the cell. In order to make the most effective use of the limited computing resources, it is usually necessary to compare the importance of each signal, determine its priority, and give priority to re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06N3/00
CPCG06N3/002
Inventor 陈梅
Owner MINZU UNIVERSITY OF CHINA
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