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Method for measuring content of chloramphenicol based on biosensor of glucometer

A biosensor, chloramphenicol technology, applied in the field of molecular biology, can solve problems such as inability to realize real-time detection on site, and achieve the effects of excellent signal amplification performance, simple detection equipment, and strong specific recognition ability

Pending Publication Date: 2021-12-07
NANCHANG HANGKONG UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The problem to be solved by the present invention is to provide a method for determining the content of chloramphenicol based on a biosensor based on a blood glucose meter, which solves the problem of antibiotic detection in the prior art requiring the use of large instruments, complex sample pretreatment, Unable to realize on-site real-time detection and other problems

Method used

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  • Method for measuring content of chloramphenicol based on biosensor of glucometer
  • Method for measuring content of chloramphenicol based on biosensor of glucometer
  • Method for measuring content of chloramphenicol based on biosensor of glucometer

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Embodiment Construction

[0044] The implementation of the present invention will be described in detail below with reference to the drawings and examples, so as to fully understand and implement the implementation process of how to use technical means to solve technical problems and achieve technical effects in the present invention.

[0045]A method for determining the content of chloramphenicol based on a blood glucose meter biosensor, specifically according to the following steps:

[0046] Step S1: Synthesis of AuNPs

[0047] First, soak the glassware used in aqua regia, rinse with ultrapure water, and dry it for later use. Then 2 mL of 1% (w / w, 34 mM) sodium citrate solution was added to 50 mL of boiling 0.01% (w / w, 0.24 mM) HAuCl 4 in solution. The solution gradually changed from purple to wine red.

[0048] Step S2: Synthesis of Aminated Magnetic Particles (MNPs)

[0049] [1,6-hexanediamine (6.5g) + anhydrous sodium acetate (2g) + FeCl 3 .6H 2 O (1.0g)] was added to 30mL ethylene glycol so...

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Abstract

The invention discloses a method for determining the content of chloramphenicol based on a biosensor of a glucometer, which comprises the following steps: connecting a specific complementary chain DNA sequence to the surface of a magnetic particle through biotin-avidin interaction, and releasing the specific DNA sequence through the specific action of chloramphenicol and an aptamer, and initiating a cyclic self-assembly process of a hairpin structure modified with gold nanoparticles / amylase, and measuring the content of chloramphenicol by utilizing magnetic separation and collection and combining with a glucometer. The preparation method comprises the following specific steps: S1, synthesizing AuNPs; S2, synthesizing aminated magnetic particles (MNPs); S3, modifying the MNPs (MNPs / tDNA / Aptamer); S4, carrying out DNA hairpin pretreatment (H1 and H2); S5, marking the NDA and glucoamylase (AuNPs / H1 / E, AuNPs / H2 / E) by using the AuNPs; S6, measuring chloramphenicol based on a glucometer; and S7, selectively investigating the content of chloramphenicol based on a glucometer. According to the method, the problem of measuring the content of chloramphenicol by using a glucometer is solved, and the detection requirement of the public on chloramphenicol is met.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a method for measuring the content of chloramphenicol with a biosensor based on a blood glucose meter. Background technique [0002] Chloramphenicol (2,2-dichloro-N-[1,3-dihydroxy-1-(4nitrophenyl)propan-2-yl]acetamide), also known as chloramphenicol, is a widely used Spectrum antibiotic drugs. Can effectively inhibit Gram-negative bacteria, Gram-positive bacteria and other pathogenic bacteria. It has very obvious effect and strong bactericidal ability. But the excessive use of this antibiotic will also damage human health. Chloramphenicol is absorbed into the human body through food and medicine, and it is easy to accumulate in the liver, thereby causing many serious side effects on humans, such as leukemia, gray baby syndrome and regeneration. Obstructive anemia. The harm of chloramphenicol to human health has attracted great attention from international organizati...

Claims

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Application Information

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IPC IPC(8): G01N33/535G01N33/543G01N33/58
CPCG01N33/9446G01N33/54326G01N33/54346G01N33/581G01N33/535
Inventor 司艳美李艳丽张书渠
Owner NANCHANG HANGKONG UNIVERSITY
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