The invention provides an
analysis method for screening
fetus trisomy 21 syndrome, and belongs to the technical field of inspection and detection.According to the method, methylated HLCS, C21orf25 and RASSF1A genes from
placenta are detected at the same time, and the method comprises the following steps that
methylation sensitive
restriction enzyme cutting is conducted, specifically, after maternal
peripheral blood is collected,
DNA is extracted, and unmethylated CGCG loci are selectively
cut through BstUI; light-driven amplification: carrying out isothermal amplification under the
irradiation of an external coaxial reflection LED
light source by utilizing a
photosensitizer Bi2MoO6 (at) NiS, and initiating a multiple light-driven LAMP reaction by taking
placenta-derived methylated HLCS, C21orf25 and RASSF1A genes in maternal
peripheral blood as templates respectively, so as to obtain a double-
chain structure DNA amplification product; detecting by using photo-
thermal test paper: modifying Bi2MoO6 (at) NiS by using three
nucleic acid chains to obtain three photo-thermal
signal probes; and mixing the amplification product with a photo-thermal
signal probe, and carrying out photo-thermal temperature
change detection to obtain a photo-thermal temperature change
signal positively correlated with the content of the amplification product. According to the
analysis method disclosed by the invention, the simultaneous amplification and efficient detection of the HLCS, C21orf25 and RASSF1A genes of the
placenta specific genes, namely the highly methylated CGCG site are realized.