The invention relates to a
heparin sodium purification technology. The crude
heparin sodium purification technology sequentially comprises the following steps: 1, dissolving crude
heparin sodium in a sodium
chloride solution, and adding an
alkaline protease for
enzymatic hydrolysis; 2, inactivating, adding diatomite, carrying out high speed
centrifugation, and removing insoluble impurities; 3, adding polysilicate, carrying out high speed
centrifugation, and removing insoluble impurities; 4, adding
alcohol for precipitating, and removing supernatant
alcohol; 5, dissolving in the sodium
chloride solution, adding
hydrogen peroxide for oxidizing, carrying out high speed
centrifugation after oxidation, and removing insoluble impurities; 6, adding
alcohol for precipitating, and removing supernatant alcohol; and 7, filtering, and carrying out vacuum lyophilizing to obtain refined
heparin sodium. The crude
heparin sodium purification technology has the advantages of low content of proteins in the refined
heparin sodium, and high yield of the refined heparin sodium.