Preparation of CCP polypeptide series and its use

A polypeptide sequence and sequence technology, applied in the field of diagnostic kits for rheumatoid arthritis, can solve the problems of lack of sensitivity and specificity, difficult differential diagnosis, unfavorable early diagnosis, etc., achieve strong specificity, low sensitivity, and improve early diagnosis rate effect

Inactive Publication Date: 2004-04-28
昆明广博生物技术有限公司
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Problems solved by technology

[0009] The above four methods lack sensitivity (40-50%) and specificity (50-60%) in the diagnosis of rheumatoid arthritis, which

Method used

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Experimental program
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Embodiment 1

[0027] The preparation of the CCP polypeptide sequence of the present invention is carried out according to the following steps:

[0028] 1) According to the primary structure of filaggrinr, calculate the hydrophilicity value of amino acids, the water treatment index and the frequency of advanced structures, calculate the most antigenic site according to the protein structure, and select the sequence of the synthetic peptide chain, namely: SHQESTXGRSRGRSGRSGS (19 peptides).

[0029] 2) The target peptide chain was synthesized by the Fmoc chemical method, and the sequence of the target peptide was: HQCHQESTXGRSRGRSGRSGS (21 peptides).

[0030] The ELISA method for detecting rheumatoid arthritis using CCP polypeptide as an antigen is:

[0031] The CCP polypeptide is used to prepare a diagnostic kit for rheumatoid arthritis, and its preparation method is as follows:

[0032] (1) Coat the CCP polypeptide (antigen) prepared above on a 96 microwell plate at a concentration of 10 μ...

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Abstract

The present invention relates to CCP polypeptide and its application. CCP polypeptide is prepared through the following steps: calculating the hydrophilicity value, water treatment index and higher structure emergence frequency of amino acid, calculating the site with maximum antigenicity based on protein structure and thus obtaining synthetic peptide chain sequence SHQESTXGRSRGRSGRSGS; synthesizing in Fmoc chemical synthesis process with the said peptide chain the destination peptide of sequence HQCGQESTXGRSRGRSGRSGS. The enzyme-linked immunological method with the CCP peptide as antigen to detect rheumatoid arthritis is provided. The rheumatoid arthritis diagnosing kit has high diagnosis specificity, up to 80 %. The present invention has high early diagnosis rate on rheumatoid arthritis.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to the preparation of CCP polypeptide series and its application to rheumatoid arthritis diagnostic kits. Background technique [0002] Rheumatoid arthritis is a teratogenic autoimmune disease. Irreversible bone and joint damage can occur within two years of onset. Recently, it is believed that early treatment can alleviate and control the disease, reduce bone and joint damage and improve prognosis. The current diagnostic criteria for rheumatoid commonly used are the following: [0003] 1. Determination of rheumatoid factor (RF) [0004] (1) Latex agglutination test: RF is an anti-human denatured IgG antibody that mainly occurs in patients with rheumatoid arthritis, and can bind to the FC segment of IgG. The denatured IgG is coated on the polystyrene latex particles, and when the sensitized latex meets the RF in the serum to be tested, agglutination can occur visible to the naked...

Claims

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Application Information

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IPC IPC(8): C07K11/00G01N33/68
Inventor 张杰
Owner 昆明广博生物技术有限公司
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