Kit for assisting in diagnosing hematogenous disseminated pulmonary tuberculosis

A technique of hematogenous dissemination and auxiliary diagnosis, which is applied in the field of molecular biology to achieve the effects of easy promotion, simple operation and high diagnostic specificity

Active Publication Date: 2019-04-12
BEIJING TUBERCULOSIS & THORACIC TUMOR RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no molecular diagnosis method for the identification of hematogenous disseminated pulmonary tuberculosis in the world, so relevant research is urgently needed to fill this gap

Method used

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  • Kit for assisting in diagnosing hematogenous disseminated pulmonary tuberculosis
  • Kit for assisting in diagnosing hematogenous disseminated pulmonary tuberculosis

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Experimental program
Comparison scheme
Effect test

Embodiment

[0034] All subjects were patients who visited Beijing Chest Hospital Affiliated to Capital Medical University and were diagnosed (according to the People's Republic of China's health industry standards newly issued by the National Health and Family Planning Commission in 2017), and they were volunteers who gave informed consent. The secondary pulmonary tuberculosis group (TB) consisted of 56 patients with secondary pulmonary tuberculosis. The hematogenous disseminated pulmonary tuberculosis group (DTB) consisted of 24 patients with hematogenous disseminated pulmonary tuberculosis. All subjects were excluded from diabetes, HIV infection and other autoimmune diseases.

[0035] 1. Collect the fasting peripheral blood of the subject, and separate peripheral blood mononuclear cells (PBMC) from the peripheral blood.

[0036] 2. Collect peripheral blood mononuclear cells and extract total RNA.

[0037] 3. Take the total RNA and perform reverse transcription to obtain cDNA.

[0038...

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Abstract

The invention discloses a kit for assisting in diagnosing hematogenous disseminated pulmonary tuberculosis. The present invention also includes applications of a substance for detecting microRNA-451aabundance, a substance for detecting microRNA-451a relative level, a substance for detecting microRNA-451a gene, a substance for detecting microRNA-451a gene expression level, and a substance for detecting microRNA-451a gene relative expression level in preparing a kit. The purpose of the kit is to diagnose or assist in diagnosing hematogenous disseminated pulmonary tuberculosis; to diagnose or assist in diagnosing hematogenous disseminated tuberculosis from pulmonary tuberculosis; and to identify or assist in identifying secondary pulmonary tuberculosis and hematogenous disseminated pulmonarytuberculosis. The inventor of the present invention find in research work that microRNA-451a is associated with the occurrence of hematogenous disseminated pulmonary tuberculosis, and can predict theoccurrence of hematogenous disseminated pulmonary tuberculosis and provide a reliable basis for timely adjustment of clinical treatment plans.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a kit for auxiliary diagnosis of hematogenous disseminated pulmonary tuberculosis. Background technique [0002] Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis (M.tb) infection. One-third of the world's population is infected, and there are about 9 million new cases every year. Therefore, tuberculosis is still a threat to the public. One of the biggest killers of health. After M.tb infects the body, it can remain latent for a long time. When it becomes ill, it mainly infects the lungs. In addition, tuberculosis can affect various organs and tissues of the body except nails, hair and teeth. Hematogenously disseminated pulmonary tuberculosis is caused by Mycobacterium tuberculosis invading the blood circulatory system and colonizing the lungs with the blood, resulting in acute onset and high fatality rate. When the bacteria spread to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 王伟刘浩然赵玲娟李传友
Owner BEIJING TUBERCULOSIS & THORACIC TUMOR RES INST
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