Hepatitis B virus surface antigen detection particles, preparation thereof and use thereof

Abstract

The invention relates to a reagent for the diagnosis of hepatitis B and discloses hepatitis B surface antigen detection particles, which are luminous particles coated by a hepatitis B surface antigen.The invention also discloses the preparation and use of the hepatitis B surface antigen detection particles. In addition, the invention also discloses an in-vitro diagnostic kit for determining the hepatitis B surface antigen in a sample and also discloses the using method of the kit. The kit of the invention can be used in combination with other serums and clinic information to diagnose the acute or chronic hepatitis B infection condition of an individual and can also be used for screening hepatitis B in perinatal females to judge the hepatitis B infection risk of the newborns.

Description

technical field

[0001] The invention relates to a diagnostic reagent for hepatitis B, in particular to a detection particle for hepatitis B virus surface antigen (HBsAg) based on the principle of light-induced chemiluminescence, its preparation and application. Background technique

[0002] Immunological detection is a means of detection based on the specific reaction of antigens and antibodies. Because it can use isotopes, enzymes, chemiluminescent substances, etc. to amplify and display the detected signals, it is often used to detect proteins, hormones, etc. trace bioactive substances.

[0003] Immunological testing in my country has basically experienced radioimmunoassay (emerged in the 1970s, and is still widely used in hospitals above the county level); enzyme-linked immunoassay (emerged in the 1980s, commonly used in various clinical institutions); Photobiological labeling and immunoassay technology represented by luminescence (began to be popularized and used in the ...

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