Immunomagnetic composite, preparation method thereof, antigen capture reagent, kit and system comprising same, and applications

Abstract

The invention discloses an immunomagnetic composite, a preparation method thereof, an antigen capture reagent, a kit and a system comprising same, and applications. The immunomagnetic composite includes: amino-functionalized magnetic microspheres formed by modifying magnetic microspheres with an amino polymer, and agglutinin jointed with the amino polymer. By means of the technical scheme, the agglutinin is actually connected to the magnetic microspheres through induction by the amino polymer coating the surfaces of the magnetic microspheres, wherein the surfaces of the magnetic microspheres are modified by a large quantity of amino groups by coating the magnetic microspheres with the amino polymer. The outer polymer has a dendritic network structure, so that conjugation load is fully increased, and steric hindrance is reduced. In addition, the target conjugate, agglutinin, is far away from the rigid surface of the magnetic microspheres, so that bioactivity of the target conjugate is guaranteed as most as possible.

Description

technical field

[0001] The present invention relates to the technical field of biomedicine, in particular, to an immunomagnetic complex, a preparation method thereof, an antigen capture agent comprising the same, a kit, a system and an application thereof. Background technique

[0002] Primary liver cancer (primary hepatic carcinoma, PHC) is a common malignant tumor, and its mortality rate ranks third in all tumors, second only to lung cancer and gastric cancer. The number of people who die of liver cancer in my country accounts for 45% of the world's deaths every year . In recent years, it is generally believed that the occurrence of PHC is the result of long-term effects of multiple factors, multiple channels, and multiple steps, including external environmental carcinogenic factors (parasites, bacteria, virus infection, aflatoxin intake, water pollution, smoking, drinking, etc.) ) and hereditary factors. Early diagnosis of PHC is particularly important because of its ins...

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