High-stability novel coronavirus spike protein, related biological material, application of related biological material, detection test paper and detection kit

Abstract

The invention discloses a high-stability novel coronavirus spike protein, a related biological material, application of the related biological material, detection test paper and a detection kit, relates to the field of biotechnology and biomedicine. The bottleneck of the industry is broken through through internal crosslinking modification on the optimal antigen spike protein, namely S protein, onthe surface of novel coronavirus, The high-stability full-length S protein trimer is obtained through expression and purification in mammals, all antigen epitopes of the new coronavirus surface spikeprotein are reserved to the maximum extent, the stable S protein serves as an antigen to capture a new coronavirus antibody in a patient sample, and a novel coronavirus antibody instant detection kitwith high accuracy, high sensitivity and high stability is produced.

Description

technical field [0001] The present invention relates to the fields of biotechnology and biomedicine, in particular to a highly stable novel coronavirus spike protein, related biological materials and applications thereof, detection test strips and detection kits, and to a stable S protein novel coronavirus based on internal cross-linking. Quantitative real-time detection kits for viral antibodies, specifically applying the best antigen stable full-length S protein on the surface of the new coronavirus, colloidal gold and nano-magnetic particle technology, to realize the anti-new coronavirus S protein IgM antibody and (or) in the blood Test strips and kits for point-of-care detection of IgG antibodies. Background technique [0002] Novel coronavirus pneumonia (COVID-19) is an infectious disease caused by infection with a new type of coronavirus (SARS-CoV-2). [0003] The novel coronavirus is highly transmissible, has a long incubation period, and is generally susceptible to ...

AI Technical Summary

Problems solved by technology

However, due to the poor stability of S protein after purification, it cannot be stored for a long time, so the immunological detection reagents currently on the market use N protein or a part of S protein (such as the receptor binding region RBD)

These reagents cannot fully characterize the antigenic properties of the virus surface, and cannot guarantee the stability of the antigenic protein in the kit reserve, and have many problems such as poor specificity and high false positives, and cannot meet the epidemic prevention and control requirements for large-scale investigation of suspected cases and asymptomatic patients. control requirements

Images