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Immunochromatographic test strip for semi-quantitatively and simultaneously detecting cTnI and Myo and preparation method thereof

An immunochromatographic test paper, semi-quantitative technology, applied in biological testing, analytical materials, measuring devices, etc., can solve the problems of narrow linear range and low sensitivity of polyproteins, and achieve good specificity, high sensitivity, and good application prospects Effect

Active Publication Date: 2012-01-18
SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a semi-quantitative immunochromatographic test strip for simultaneously detecting cTnI and Myo and a preparation method thereof. The test strip detects two proteins with large differences in abundance at the same time. The rapid diagnosis of myocardial infarction provides great convenience, solves and makes up for the low sensitivity of traditional immune test strip technology, and the defects of narrow linear range when detecting polyproteins at the same time

Method used

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  • Immunochromatographic test strip for semi-quantitatively and simultaneously detecting cTnI and Myo and preparation method thereof
  • Immunochromatographic test strip for semi-quantitatively and simultaneously detecting cTnI and Myo and preparation method thereof
  • Immunochromatographic test strip for semi-quantitatively and simultaneously detecting cTnI and Myo and preparation method thereof

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Embodiment 1

[0030] 1) Soak the sample pad in the sample pad treatment solution for 30 minutes, take it out and dry it in a 37°C drying oven for 12 hours, then add a desiccant and store it for later use; the sample pad treatment solution contains 1% BSA, 0.05% Tween-20, 0.05% sodium azide in 10 mMPBS.

[0031] 2) Using 10mM, pH 7.4 PBS, adjust the concentration of cTnI-coated antibody, Myo-coated antibody and goat anti-mouse IgG antibody to 1mg / ml, respectively, and print the three on the nitrocellulose membrane evenly, and then in 37 ℃ drying oven for 12 hours, add desiccant and seal for later use.

[0032] 3) Using a nano-gold solution with a diameter of 13nm, using 0.2M K 2 CO 3 Adjust the pH of the solution to 8.5, add cTnI capture antibody to adjust the final concentration to 8 μg / ml, let it stand at room temperature for 30 minutes, then add biotinylated single-stranded DNA to make the final concentration 1 μM, and let it stand at room temperature for 16 hours. Then add a final con...

Embodiment 2

[0055] 1) After soaking the sample pad in the sample pad treatment solution for 60 minutes, take it out and dry it in a drying oven at 45°C for 18 hours, then add a desiccant and store it for later use; the sample pad treatment solution contains 1% BSA, 0.05% Tween- 20, 0.05% sodium azide in 10 mM PBS.

[0056] 2) Using 10mM, pH 7.4 PBS, adjust the concentration of cTnI-coated antibody, Myo-coated antibody and goat anti-mouse IgG antibody to 1mg / ml, respectively, and print the three evenly on the nitrocellulose membrane, and then in 45 ℃ drying oven for 18 hours, adding a desiccant and storing for later use.

[0057] 3) Using a nano-gold solution with a diameter of 13nm, using 0.2M K 2 CO 3 Adjust the pH value of the solution to 8.5, add cTnI capture antibody to adjust the final concentration to 8 μg / ml, let it stand at room temperature for 30 minutes, then add biotinylated single-stranded DNA to make the final concentration 1 μM, and let it stand at room temperature for 16 ...

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Abstract

The invention relates to an immunochromatographic test strip for semi-quantitatively and simultaneously detecting cTnI and Myo and a preparation method thereof. The test strip comprises a base plate, a nitrocellulose membrane, a first bonding pad, a second bonding pad, a sample pad and a water absorbing pad. According to the preparation method, the pretreated nitrocellulose membrane, first bonding pad, second bonding pad, sample pad and water absorbing pad are sequentially and mutually staggered and attached to the base plate so as to obtain the immunochromatographic test strip for simultaneously detecting the cTnI and the Myo. The immunochromatographic test strip disclosed by the invention can be used for simultaneously detecting two proteins with greater abundance difference, providing great convenience for quick diagnosis of clinical myocardial infarction and overcoming and making up the defects of low sensitiveness and narrow linear range during detection of polyprotein in a traditional immunochromatographic test strip technology. The preparation method has the advantages of simple process, low cost, simpleness and convenience in operation and favorable application prospect.

Description

technical field [0001] The invention belongs to the detection field of cTnI and Myo, in particular to an immunochromatographic test strip for semi-quantitatively detecting cTnI and Myo simultaneously and a preparation method thereof. Background technique [0002] Acute myocardial infarction (AMI) is an emergency and severe disease of the cardiovascular system, with high morbidity, poor prognosis and high mortality. Early and rapid diagnosis is of great significance for the timely treatment and prognosis of the disease, and it is an important research direction in laboratory medicine. In the early diagnosis of acute myocardial infarction, the joint detection of troponin I (cTnI) and myoglobin (Myo) has important diagnostic significance. cTnI is a cardioregulatory protein present in the myofilament of cardiomyocytes, and it is a more specific protein when AMI occurs. Its content in normal blood is very low, about 20.4pg / ml. Its concentration began to increase at 2.2-6.8 hou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/531
CPCG01N2333/805G01N33/558G01N33/68G01N33/531G01N33/6887G01N33/588B82Y15/00G01N33/54388
Inventor 毛红菊祝继敏金庆辉赵建龙朱大年
Owner SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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