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Method for detecting aflatoxin through immunosensor

An immune sensor and aflatoxin technology, applied in the medical field, can solve difficult problems and achieve the effect of improving electrochemical performance and good electrochemical conductivity

Inactive Publication Date: 2017-01-11
HONGHE COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, it has been found that the surface of graphene is inert, has high chemical stability, and there is a strong van der Waals force between graphene, which is easy to aggregate and difficult to dissolve in water and other solvents.

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  • Method for detecting aflatoxin through immunosensor
  • Method for detecting aflatoxin through immunosensor
  • Method for detecting aflatoxin through immunosensor

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Embodiment Construction

[0054] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0055] The application principle of the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0056] A method for detecting aflatoxin by an immunosensor, the method for detecting aflatoxin by the immunosensor comprises:

[0057] S101: The prepared Au / PANI / GN is modified on the surface of the gold electrode, and the electrochemical performance of the electrode is characterized by AC impedance method and cyclic voltammetry;

[0058] S102: Construction of Aspergillus flavus immunosensor, drop-coating Au / PANI / GN composite nanomaterials on the surface of gold...

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Abstract

The invention discloses a method for detecting aflatoxin through an immunosensor. The aflatoxin (AFT) is a fungal toxin which has the high carcinogenicity, teratogenicity and toxicity, and accurate and rapid analysis on the aflatoxin is one of most effective means for reducing and avoiding harm of the aflatoxin. A prepared gold-polyaniline-graphene (Au-PANI-GN) composite nanomaterial is represented by adopting an ultraviolet spectrum and a transmission electron microscope and used for constructing the electrochemical immunosensor, the electrochemical immunosensor is represented by adopting a cyclic voltammetry method, an electrochemical AC impedance method and a square wave voltammetry method, and the prepared electrochemical immunosensor detects the aflatorxin B1. Experiment results show that the constructed electrochemical immunosensor has the good electrochemical activity on detection of the aflatoxin, and the concentrations of the aflatoxin and peak currents have the good linear relation, wherein the linear equation of the relation is y=-0.177x+4.28.

Description

technical field [0001] The invention belongs to the field of medicine, in particular to a method for detecting aflatoxin by an immunosensor. Background technique [0002] Overview of Electrochemical Immunosensors [0003] Electrochemical immunosensor is a commonly used analysis method in immunoassay methods. Through the specific binding and recognition between antibodies and antigens, electrodes are used as sensing elements to form immune complexes loaded on the surface of electrodes, and Quantitative detection and research of analytes. Immunosensors have many advantages, such as fast and convenient detection, high sensitivity, low price, good selectivity, strong specificity, suitable for on-line detection, and can realize the detection of actual samples. So far, immunosensors have important applications in fields such as environmental inspection, clinical medicine, and food safety industry. [0004] Overview of Aflatoxins [0005] Aflatoxin is a strong toxin, so far, th...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/416
CPCG01N27/3278G01N27/4163
Inventor 石玲刘卫王泽锋陈显兰杨光明苟高章徐世娟
Owner HONGHE COLLEGE
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