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Preparation method and application of co-immobilized enzyme electrode used for detecting p-dihydroxybenzene and o-dihydroxybenzene

A technology of hydroquinone and catechol, which is applied in the field of co-immobilized enzyme electrode preparation for detecting hydroquinone and catechol, can solve the problems of high detection limit and inability to detect sensitive pollutants, and achieve Low detection limit, high sensitivity and good selectivity

Inactive Publication Date: 2014-08-27
HOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These electrodes can detect HQ and CC, but their detection limits are relatively high, and they cannot be sensitively detected for trace pollutants in the environment

Method used

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  • Preparation method and application of co-immobilized enzyme electrode used for detecting p-dihydroxybenzene and o-dihydroxybenzene
  • Preparation method and application of co-immobilized enzyme electrode used for detecting p-dihydroxybenzene and o-dihydroxybenzene
  • Preparation method and application of co-immobilized enzyme electrode used for detecting p-dihydroxybenzene and o-dihydroxybenzene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Preparation of tyrosinase / peroxidase-graphene oxide-chitosan / glassy carbon electrode (Tyr / Hrp-GO-Chit / GCE)

[0044] Activation treatment of GCE: In the electrochemical workstation, Ag / AgCl is used as the reference electrode, the platinum electrode is used as the counter electrode, and GCE is used as the working electrode, and the 0.5M sulfuric acid solution is scanned at a scan rate of 100mV / s at a potential of -1.0-1.0V. Scan 20 laps. The activated electrodes were left to dry at room temperature.

[0045] 10 mg of GO was stirred in 40 mL of concentrated sulfuric acid and nitric acid mixture (3:1) for 1.5 h, put into concentrated hydrochloric acid and sonicated for 1.5 h, filtered and washed with deionized water, and dried at room temperature.

[0046] Dissolve 10 mg of carboxylated GO in 100 mL of SOCl 2 The mixed solution of DMF and DMF (20:1) was stirred in a water bath for 24 hours at a temperature of 70°C. The resulting acid-chlorinated graphene was filtered, wa...

Embodiment 2

[0053] Sensitivity, detection limit and detection range of Tyr / Hrp-GO-Chit / GCE for HQ and CC detection

[0054] Configuration pH is the phosphate buffer solution of 6.5, this solution is under agitation, and the Tyr / Hrp-GO-Chit / GCE that prepares with embodiment 1 is made working electrode, and platinum electrode is made counter electrode, and Ag / AgCl is made reference electrode, The solution was scanned using chronoamperometry.

[0055] image 3 The chronoamperometry graph of the buffer solution in which HQ was continuously added dropwise was scanned by chronoamperometry for the Tyr / Hrp-GO-Chit / GCE electrode (the inset is the fitting curve between the current value and the concentration of HQ (I(HQ)=-0.0222C(HQ )-1.5854)), the operating voltage is -0.13V. According to the graph analysis, the linear range of Tyr / Hrp-GO-Chit / GCE to detect HQ is 3-200μM, and the sensitivity is 22.2μAmM -1 , the limit of detection was 1.26 μM.

[0056] Figure 4 The chronoamperometric diagram...

Embodiment 3

[0058] Preparation of Tyr / Hrp-GO-Chit / GCE

[0059] In the electrochemical workstation, Ag / AgCl was used as the reference electrode, the platinum electrode was used as the counter electrode, and the GCE was used as the working electrode. The 0.1M sulfuric acid solution was scanned at a scan rate of 50mV / s at a potential of -1.0-1.0V for 10 cycles. The activated electrodes were left to dry at room temperature.

[0060] 5 mg of GO was stirred in 20 mL of concentrated sulfuric acid and nitric acid mixture (2:1) for 1 h, put into concentrated hydrochloric acid and sonicated for 1 h, filtered and washed with deionized water, and dried at room temperature.

[0061] Dissolve 5 mg of carboxylated GO in 50 mL of SOCl 2 And the mixed solution of DMF (10:1) was stirred in a water bath for 12h at a temperature of 60°C. The resulting acid-chlorinated graphene was filtered, washed with anhydrous tetrahydrofuran, and vacuum-dried at room temperature.

[0062] 5 mg of acid-chlorinated GO wa...

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Abstract

The invention provides a preparation method and an application of a co-immobilized enzyme electrode which is used for detecting p-dihydroxybenzene and o-dihydroxybenzene. The preparation method comprises steps of: immobilizing tyrosinase and peroxidase on oxidized grephene to obtain a co-immobilized enzyme liquid and smearing dropwisely the co-immobilized enzyme liquid and a chitosan solution successively on a surface of a glassy carbon electrode to obtain the co-immobilized enzyme electrode which is used for detecting p-dihydroxybenzene and o-dihydroxybenzene. The electrode is low in cost, is easy and convenient to operate, is quick and sensitive, is low in detection limit, is good in selectivity and can be used for detecting microscale p-dihydroxybenzene and o-dihydroxybenzene in environment.

Description

technical field [0001] The invention belongs to the field of environmental detection, and in particular relates to a preparation method and application of a co-immobilized enzyme electrode for detecting hydroquinone and catechol. Background technique [0002] Catechol (Catechol, CC) and hydroquinone (Hydroquinone, HQ), as important chemical raw materials, are widely used in rubber anti-aging agents, photographic developers and spices, pesticides, medicines, dyes and other fields. Larger than common phenolic pollutants, HQ and CC are extremely harmful to animals, plants and the aquatic environment even at extremely low concentrations. In order to assess the risks to actual human health due to exposure to HQ and CC, accurate data on their concentrations in the environment at very low concentration levels are required. Therefore, it is of great significance to develop an analytical method for the simple and sensitive detection of trace amounts of HQ and CC. [0003] In recent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/416
Inventor 华祖林白雪王超凡黄欣秦琴张琪张晓媛马文强顾海鑫戴章艳
Owner HOHAI UNIV
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