SERS detection kit and method for detecting SARS-CoV-2 nucleic acid

A detection kit, sars-cov-2 technology, applied in the field of functional nanomaterials and biological detection, can solve the problems of time-consuming and high detection cost, and achieve the effect of high-sensitivity detection

Active Publication Date: 2022-04-12
GUANGZHOU NAT LAB +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Although this technology has achieved great success in the detection of viral nucleic acids, there are still obvious deficiencies and limitations in the control of reaction conditions and experimental operations, which lead to problems such as high cost and time-consuming detection.
However, due to the rapid spread of COVID-19 in a short period of time, a long test backlog can have unpredictable results in the spread of the pandemic

Method used

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  • SERS detection kit and method for detecting SARS-CoV-2 nucleic acid
  • SERS detection kit and method for detecting SARS-CoV-2 nucleic acid
  • SERS detection kit and method for detecting SARS-CoV-2 nucleic acid

Examples

Experimental program
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Effect test

Embodiment 1

[0080] Example 1 Preparation of Surface-enhanced Raman Scattering Detection Kit for SARS-CoV-2 Nucleic Acid Detection

[0081] The SERS detection kit for SARS-CoV-2 nucleic acid detection of the present embodiment includes:

[0082] 1. SERS detection chip

[0083] That is, the silver nanorod array is used as the substrate, and the surface of the substrate is decorated with captured single-chain C at a concentration of 500 nM. The silver nanorod array substrate includes 3 × 10 array-type small holes, and the diameter of each small hole is 4mm, and the depth of each small hole is 1mm.

[0084] 2. The first reagent (i.e. detection probe)

[0085] It is obtained by mixing auxiliary single-stranded primer with a concentration ratio of 1:1 and hairpin DNA single-stranded H1, and annealing at 90°C to 95°C for 5 minutes, and the working concentration of the first reagent is 5 μM .

[0086] 3. The second reagent (i.e. hairpin DNA single strand H2)

[0087] Hairpin DNA single stran...

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Abstract

The invention discloses a SERS detection kit and a method for detecting SARS-CoV-2 nucleic acid. The SERS detection kit includes a SERS detection chip, a first reagent, a second reagent and a third reagent. The SERS detection kit of the present invention detects the Raman signal of the SERS probe on the SERS detection chip through the mutual cooperation of the SERS detection chip, the first reagent, the second reagent and the third reagent, so as to realize the detection of SARS-CoV-2 nucleic acid The rapid, specific and highly sensitive detection can be further applied to faster, more sensitive, multi-channel and on-site instant viral nucleic acid detection.

Description

technical field [0001] The invention belongs to the technical field of functional nanomaterials and biological detection, more specifically, the invention relates to a surface-enhanced Raman scattering (SERS) detection kit for detecting SARS-CoV-2 nucleic acid and a preparation method thereof, and a A method for detecting SARS-CoV-2 nucleic acid based on SERS. Background technique [0002] Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a newly discovered infectious coronavirus, which has caused the novel coronavirus pneumonia (COVID-19) pandemic, which has posed a huge threat to human life and health . Therefore, before developing effective drugs and vaccines to suppress the epidemic of COVID-19, early diagnosis of COVID-19 is still an important way to prevent infection at this stage. Furthermore, rapid and early detection of SARS-CoV-2 plays a crucial role for screening and diagnosis, given that COVID-19 infection can be transmitted during the incubation ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/6816C12Q1/6837C12N15/11G01N21/65
CPCC12Q1/701C12Q1/6816C12Q1/6837G01N21/658Y02A50/30
Inventor 缪夏萍张晶晶于世辉汪联辉陈娜宋春元
Owner GUANGZHOU NAT LAB
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