SERS detection kit and method for detecting SARS-CoV-2 nucleic acid
A detection kit, sars-cov-2 technology, applied in the field of functional nanomaterials and biological detection, can solve the problems of time-consuming and high detection cost, and achieve the effect of high-sensitivity detection
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[0080] Example 1 Preparation of Surface-enhanced Raman Scattering Detection Kit for SARS-CoV-2 Nucleic Acid Detection
[0081] The SERS detection kit for SARS-CoV-2 nucleic acid detection of the present embodiment includes:
[0082] 1. SERS detection chip
[0083] That is, the silver nanorod array is used as the substrate, and the surface of the substrate is decorated with captured single-chain C at a concentration of 500 nM. The silver nanorod array substrate includes 3 × 10 array-type small holes, and the diameter of each small hole is 4mm, and the depth of each small hole is 1mm.
[0084] 2. The first reagent (i.e. detection probe)
[0085] It is obtained by mixing auxiliary single-stranded primer with a concentration ratio of 1:1 and hairpin DNA single-stranded H1, and annealing at 90°C to 95°C for 5 minutes, and the working concentration of the first reagent is 5 μM .
[0086] 3. The second reagent (i.e. hairpin DNA single strand H2)
[0087] Hairpin DNA single stran...
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