A special culture medium and culture method for scaffold-free organoids of breast cancer
A culture medium and organoid technology, which is applied in the special medium and culture field of breast cancer organoids, can solve the problems of affecting the cell state, cumbersome removal of scaffold materials, poor cell growth, etc., achieving a high success rate and maintaining tumor specificity. , the effect of high specificity of drug susceptibility results
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[0060] Example 1 Culture of Breast Cancer 3D Organoids
[0061] 1. Special medium formula for breast cancer organoids
[0062] The ingredients, the concentration of each ingredient or the volume percentage content are shown in Table 1.
[0063] Table 1 Composition of special medium for breast cancer organoids
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[0066] 2. Patient Breast Cancer Tissue Processing
[0067] Tumor tissues were mainly derived from surgically resected specimens and biopsies obtained from breast cancer patients, and patients were required not to have received any antitumor therapy. In order to ensure the activity of isolated tumor primary cells, the blood clots, necrotic tissue, fat and connective tissue on the surgically resected tumor specimens were immediately cut off, and then treated with double antibodies (500 U / mL penicillin and 500 μg / mL streptavidin) prime) PBS solution, and then cut the tissue pieces to 1mm with tissue scissors 3 left and right size. The tissue ca...
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