The invention discloses a sweat induction and multi-parameter detection integrated sensor and a preparation method and application thereof, and belongs to the technical field of flexible biosensing, a PDMS flexible substrate is adopted to construct a multi-layer integrated structure, the sweat induction and multi-parameter detection integrated sensor comprises a sweat guide layer, a sweat collection layer, a detection
electrode layer and a micro-fluidic
chip layer, sweat
secretion is promoted by accurately controlling the temperature to be less than or equal to 40 DEG C; the detection
electrode layer adopts a silk-
screen printing process to prepare a three-
electrode system, comprises a specifically modified glucose
working electrode and a beta-
hydroxybutyric acid working electrode and can synchronously detect the concentration of glucose and beta-
hydroxybutyric acid in sweat, and the micro-fluidic
chip layer is integrated with a
Bluetooth transmission module to realize real-time
wireless transmission of detection data to a mobile terminal; the
biosensor has the advantages of being good in
biocompatibility, high in detection sensitivity and comfortable to wear and can tolerate bending with the curvature
radius larger than 5 mm, and a non-invasive and continuous
metabolism monitoring solution is provided for
diabetes management.