Dental plaque temperature-sensitive gel color developing agent

Abstract

The invention relates to the technical field of temperature-sensitive gel, in particular to a dental plaque temperature-sensitive gel color developing agent. The temperature-sensitive gel color developing agent for dental plaque comprises the following components in percentage by mass volume: 0.4-5% of food coloring; 0.5%-2% of a charge protective agent; 0.5%-3% of an adhesive material; 10%-55% of a temperature-sensitive material; 0.05%-0.3% of a bacteriostatic agent and a proper amount of water. The color developing agent is safe and non-toxic, is a flowing liquid at room temperature, is converted into a gel solid from a liquid state when the color developing agent is coated on the tooth surface to reach a phase transition temperature, can effectively reduce the problem of non-specific adsorption compared with a liquid color developing agent, and is easy to remove after being used.

Description

Technical Field

[0001] The present invention relates to the technical field of thermosensitive gels, in particular to a thermosensitive dental plaque gel developer. Background Art

[0002] Dental plaque is a complex three-dimensional structure composed of microbial cells from various genera, including Streptococcus, Lactobacillus, and Actinomyces, and an extracellular polymer matrix. It adheres to and colonizes the tooth surface, forming a typical bacterial biofilm. Studies have shown that bacteria present in dental plaque biofilms utilize a completely different set of genetic systems to exert their pathogenicity, resulting in enhanced drug resistance, virulence, and the ability to thwart host immune defenses compared to the same bacteria in their planktonic state.

[0003] Plaque consists of two parts: cells and an extracellular matrix. The extracellular matrix is ​​composed of proteins and extracellular polysaccharides (EPS), which enhance the adhesion and cohesion of the biofilm, protect attached bacteria, and promote the reduction of local pH to form an acidic environment. Extracellular polysaccharides constitute the external environment for the growth of biofilm bacteria and have a protective effect on biofilm bacteria. Their porous network structure helps bacteria capture surrounding nutrients to ensure bacterial metabolism in a state of starvation and also promotes adhesion between bacteria. The various bacterial species in the plaque form regular copolymerization combinations. Filamentous bacteria grow parallel to each other and perpendicular to the tooth surface, arranged in a fence-like shape. Cocci and bacilli surround them to form corncob or wheat ear-like structures. This structure helps bacteria deep in the dental plaque biofilm obtain nutrients and oxygen, while also increasing the bacterial adhesion area.

[0004] The formation of dental plaque is a continuous kinetic process in which planktonic oral bacteria adhere to the acquired pellicle on the tooth surface, grow, migrate, and reattach. When salivary glycoproteins come into contact with teeth, they adhere to the enamel surface, forming a film-like substance known as the acquired pellicle. Bacteria then grow on the surface of the acquired pellicle and produce acid, which deposits the glycoproteins. Subsequently, the bacteria grow, develop, reproduce, and die within the acquired pellicle, carrying out a complex series of metabolic activities. Plaque substances gradually mineralize into dental calculus in the salivary environment. This calculus not only compresses and irritates the gums but also absorbs bacteria and toxic substances, causing gingivitis, redness, swelling, and easy bleeding. If gingivitis is not treated promptly, it can progress to periodontitis, leading to gum recession, exposed tooth roots, and tooth sensitivity. When dental plaque expands and grows below the gum line, the body's own immune system will fight against the bacteria. The bacterial toxins and the body's own enzymes will begin to destroy the tissues around the teeth that support them. After the alveolar bone, gums and other tissues that support the teeth are destroyed, the teeth will gradually loosen and eventually fall out.

[0005] Because dental plaque is colorless and its growth is restricted by anatomical conditions, it is often impossible to completely remove it. Currently used plaque detection agents, while displaying plaque, tend to nonspecifically adsorb to the gums, tongue, and other areas of the mouth, making them difficult to remove after use. Therefore, the development of new colorants is of great significance. Summary of the Invention

[0006] The present application solves at least one of the problems of the related art from the following aspects.

[0007] The present application provides a thermosensitive gel developer for dental plaque, comprising an edible pigment, a charge protection agent, an adhesive material, a thermosensitive material, an antibacterial agent, and water. The contents of the components are as follows, expressed in percentage by mass and volume:

[0008] Food coloring: 0.4%-5%;

[0009] Charge protection agent: 0.5%-2%;

[0010] Adhesive materials: 0.5%-3%;

[0011] Temperature-sensitive materials: 10%-55%; and

[0012] Antibacterial agent: 0.05%-0.3%

[0013] The balance is water.

[0014] In some embodiments, the plaque thermosensitive gel developer comprises an edible pigment, a charge protection agent, an adhesive material, a thermosensitive material, an antibacterial agent, and water. The contents of each component are as follows, calculated by weight volume percentage:

[0015] Food coloring: 0.4%-3%;

[0016] Charge protection agent: 0.8%-1%;

[0017] Adhesive materials: 1.2%-1.6%;

[0018] Temperature-sensitive materials: 20%-30%;

[0019] Bacteriostatic agents: 0.05%-0.3%; and

[0020] The balance is water.

[0021] In some embodiments, the charge protection agent is at least one of sodium alginate, sodium chloride, and mannitol.

[0022] In some embodiments, the adhesive material is at least one of povidone K30, hydroxypropyl methylcellulose, methylcellulose, and chitosan.

[0023] In some embodiments, the antibacterial agent is at least one of sodium benzoate, potassium sorbate, ethylparaben, sorbic acid, and sodium propylparaben.

[0024] In some embodiments, the adhesive material is povidone K30 and hydroxypropyl methylcellulose.

[0025] In some embodiments, the plaque thermosensitive gel developer comprises, by weight volume percentage:

[0026] Povidone K30: 0.03%-0.06%; and

[0027] Hydroxypropyl methylcellulose: 1.2%-1.6%.

[0028] In some embodiments, the temperature-sensitive material is poloxamer 407 and poloxamer 188.

[0029] In some embodiments, the plaque thermosensitive gel developer comprises, by weight volume percentage:

[0030] Poloxamer 407: 18%-23%; and

[0031] Poloxamer 188: 4%-6%.

[0032] In some embodiments, the food pigments are erythrosine and brilliant blue, and in terms of mass volume percentage, the dental plaque thermosensitive gel developer comprises: erythrosine 0.8% and brilliant blue 0.4%; or the dental plaque thermosensitive gel developer comprises erythrosine 2% and brilliant blue 1%.

[0033] In some embodiments, the food coloring is erythrosine, and the plaque thermosensitive gel developer comprises, in terms of mass volume percentage, erythrosine 0.8%-2%.

[0034] In some embodiments, the food coloring is brilliant blue, and the plaque thermosensitive gel developer comprises, by weight volume percentage, 0.4%-1% brilliant blue.

[0035] In some embodiments, a liquid developer is prepared by using water as a solvent and adding food coloring, cellulose, alcohols, potassium salts, pH regulators, antibacterial agents, and flavors.

[0036] In some embodiments, a thermosensitive gel developer is prepared using an edible coloring, a charge protection agent, an adhesive material, a thermosensitive material, an antibacterial agent, and an appropriate amount of water. This developer achieves the following benefits: it is safe and non-toxic, is a flowing liquid at room temperature, and transforms from a liquid to a gel solid when applied to the tooth surface and reaches a phase transition temperature. Compared to liquid developers, it effectively reduces nonspecific adsorption and is easy to remove after use.

[0037] The embodiments of the present application achieve the following beneficial effects:

[0038] The thermosensitive gel color developer provided in the embodiments of the present application is safe and non-toxic, is a flowing liquid at room temperature, and when applied to the tooth surface and reaches the phase transition temperature, it changes from a liquid to a gel solid. Compared with liquid color developers, it can effectively reduce the problem of nonspecific adsorption and is easy to remove after use. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0040] Figure 1 The coating results of the commercially available developer (left) and the developer of prescription 1 (right) in Example 1 are shown.

[0041] Figure 2 The results of the elution test of the commercially available developer in Example 1 are shown.

[0042] Figure 3 The results of the elution test of the color developers of Prescription 1 (left), Prescription 2 (middle) and Prescription 3 (right) in Example 1 are shown.

[0043] Figure 4 The results are a comparison of the nonspecific adsorption and elution of the thermosensitive gel prepared by adding 0.1% sodium benzoate, an antibacterial agent, to the fourth group of formulations in Table 3 of Example 2 and a commercially available color developer. DETAILED DESCRIPTION

[0044] The present invention will be further described in detail below in conjunction with specific embodiments. The examples provided are only for illustrating the present invention and are not intended to limit the scope of the present invention. The examples provided below can serve as a guide for further improvements by those skilled in the art and are not intended to limit the present invention in any way.

[0045] This application is made based on the following knowledge of the inventors:

[0046] Poloxamer, a typical example of a nonionic in situ gel matrix, is marketed as Pluronic and is a widely used temperature-sensitive in situ gel matrix. Poloxamers are nonionic polymers composed of alternating polyoxyethylene (PEO) and polyoxypropylene (PPO) chains. Generally, poloxamer molecules exhibit a PEO-PPO-PEO block structure. The gelling properties of poloxamer molecules are influenced by many factors, the most important being the relative ratio of the PPO and PEO blocks within the molecule. Adjusting the ratio of the two blocks can effectively alter the relevant gelling parameters, resulting in superior in situ gel matrix materials. Therefore, poloxamers represent a family of polymers with distinct compositions and properties. Although all belong to the poloxamer class, the ability to manipulate the ratio of the two blocks results in significant compositional differences. These are distinguished by numerical designations, such as P407 and P188. The suffix number clearly indicates the average molecular weight and the proportion of the PEO block within the molecule. Poloxamers are exceptional in situ gel matrices and are currently the mainstream of temperature-sensitive in situ gel matrices. P407 and P188 are the most widely used. P407 is typically used at a concentration of 20%-30%, exhibiting excellent temperature-sensitive gelling properties (gelling with increasing temperature) within this concentration range.

[0047] Research has found that due to the differences in the physicochemical properties of the polyoxyethylene and polyoxypropylene blocks that comprise the poloxamer block, poloxamer exhibits unique gelling properties in water. Specifically, as temperature rises, the relatively hydrophobic polyoxypropylene blocks within the poloxamer molecule form a structure in which the hydrophobic effect of the hydrophobic PPO is encapsulated within the core, while the relatively hydrophilic PEO, after binding with water, entangles and surrounds the hydrophobic structure, forming micelles of varying sizes but similar composition. These micelles in solution do not exist as completely independent entities. When the solution concentration exceeds a certain value, various inter-micelle forces, under certain environmental conditions, can cause further entanglement, stacking, and aggregation of the poloxamer molecules within the micelle state. At this point, if the temperature rises further, gelation occurs, resulting in the formation of an in situ gel, making it suitable for use as a temperature-sensitive in situ gel matrix.

[0048] Various poloxamers have varying ratios of the two blocks, resulting in significant differences in their physicochemical properties and various parameters. To achieve the desired gelation effect, such as achieving a specific gelation temperature and viscosity, minimizing adverse reactions, and achieving good drug release characteristics, it is necessary to prepare a gel matrix by mixing two or more poloxamer polymers in varying proportions. Although poloxamer 407 is currently the most widely used poloxamer gel matrix, it has certain limitations. For example, it is difficult to achieve an ideal gelation temperature when used alone. Furthermore, it suffers from deficiencies in mechanical strength, dissolution properties, and drug release. Therefore, it is typically mixed with poloxamer 188 in appropriate proportions, depending on the specific application requirements. This mixed gel matrix exhibits superior performance in terms of gelation temperature, mechanical strength, and dissolution properties, surpassing the effects of either poloxamer when used alone.

[0049] In a first aspect, an embodiment of the present application provides a thermosensitive gel developer for dental plaque, comprising an edible pigment, a charge protection agent, an adhesive material, a thermosensitive material, an antibacterial agent, and water, wherein the contents of the components are as follows, in terms of mass volume percentage:

[0050] Food coloring: 0.4%-5% (e.g., 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.3%, 1.2%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2.0%, 2.1%, 2.2%, 2.3%, 2.4%, 2.5%, 2.6%, 2.7%, 2.8%, 2.9%, 3.0%, 3.1%, 3.2%, 3.3%, 3.4%, 3.5%, 3.6%, 3.7%, 3.8%, 3.9%, 4.0%, 4.1%, 4.2%, 4.3%, 4.4%, 4.5%, 4.6%, 4.7%, 4.8%, 4.9%);

[0051] Charge protectant: 0.5%-2% (e.g., 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.3%, 1.2%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%);

[0052] Adhesive materials: 0.5%-3% (e.g., 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.3%, 1.2%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2.0%, 2.1%, 2.2%, 2.3%, 2.4%, 2.5%, 2.6%, 2.7%, 2.8%, 2.9%);

[0053] Temperature-sensitive materials: 10%-55% (e.g., 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%); and

[0054] Antibacterial agent: 0.05%-0.3% (e.g., 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.11%, 0.12%, 0.13%, 0.14%, 0.15%, 0.16%, 0.17%, 0.18%, 0.19%, 0.2%, 0.21%, 0.22%, 0.23%, 0.24%, 0.25%, 0.26%, 0.27%, 0.28%, 0.29%)

[0055] The balance is water.

[0056] In some embodiments, the plaque thermosensitive gel developer comprises an edible pigment, a charge protection agent, an adhesive material, a thermosensitive material, an antibacterial agent, and water. The contents of each component are as follows, in terms of mass volume percentage:

[0057] Food coloring: 0.4%-3%;

[0058] Charge protection agent: 0.8%-1%;

[0059] Adhesive materials: 1.2%-1.6%;

[0060] Temperature-sensitive materials: 20%-30%;

[0061] Bacteriostatic agents: 0.05%-0.3%; and

[0062] The balance is water.

[0063] In some embodiments, the charge protection agent is at least one of sodium alginate, sodium chloride, and mannitol.

[0064] In some embodiments, the adhesive material is at least one of povidone K30, hydroxypropyl methylcellulose, methylcellulose, and chitosan.

[0065] In some embodiments, the antibacterial agent is at least one of sodium benzoate, potassium sorbate, ethylparaben, sorbic acid, and sodium propylparaben.

[0066] In some embodiments, the adhesive material is povidone K30 and hydroxypropyl methylcellulose.

[0067] In some embodiments, the plaque thermosensitive gel developer comprises, by weight volume percentage:

[0068] Povidone K30: 0.03%-0.06% (e.g., 0.04%, 0.05%); and

[0069] Hydroxypropyl methylcellulose: 1.2%-1.6% (e.g., 1.3%, 1.4%, 1.5%).

[0070] In some embodiments, the temperature-sensitive material is poloxamer 407 and poloxamer 188.

[0071] In some embodiments, the plaque thermosensitive gel developer comprises, by weight volume percentage:

[0072] Poloxamer 407: 18%-23% (e.g., 19%, 20%, 21%, 22%); and

[0073] Poloxamer 188: 4%-6% (e.g., 4.1%, 4.2%, 4.3%, 4.4%, 4.5%, 4.6%, 4.7%, 4.8%, 4.9%, 5.0%, 5.1%, 5.2%, 5.3%, 5.4%, 5.5%, 5.6%, 5.7%, 5.8%, 5.9%).

[0074] In some embodiments, the food pigments are erythrosine and brilliant blue, and in terms of mass volume percentage, the dental plaque thermosensitive gel developer comprises: erythrosine 0.8% and brilliant blue 0.4%; or the dental plaque thermosensitive gel developer comprises erythrosine 2% and brilliant blue 1%.

[0075] In some embodiments, the food coloring is erythrosine, and the plaque thermosensitive gel developer comprises, in terms of mass volume percentage, erythrosine 0.8%-2%.

[0076] In some embodiments, the food coloring is brilliant blue, and the plaque thermosensitive gel developer comprises, by weight volume percentage, 0.4%-1% brilliant blue.

[0077] The various embodiments of the present application can be combined with each other, and the resulting technical solutions are still within the protection scope of the present application.

[0078] the term

[0079] Mass volume percentage refers to the proportion of the mass of solute in a volume of solution. This ratio is usually expressed as a percentage and is calculated as: Mass volume percentage = (mass of solute / volume of solution) × 100%.

[0080] Example 1 Liquid developer

[0081] 1.1 Establishment of dental plaque model

[0082] Dental plaque model animals mainly include large animals (primates, miniature pigs, dogs, etc.) and small animals (rats, hamsters, etc.). The upper and lower jaws of rodents each have two incisors and six molars. The periodontal tissue structure and pathology of rodents are similar to those of humans, and they are cheap and easy to operate. Therefore, rodents are currently the most widely used rodents for studying the pathogenesis of periodontitis. Because rough food is conducive to the self-cleaning of the body's tooth surface, and a high-sugar viscous diet is not conducive to the self-cleaning of teeth, it is conducive to the formation of dental plaque and tartar accumulation. Supragingival plaque provides bacterial attachment sites, growth conditions and a lower redox potential for subgingival plaque formation. The high-sugar diet is simple and has good repeatability. Therefore, the present embodiment adopts the high-sugar diet of Wistar rats to establish a dental plaque model.

[0083] Five-week-old male Wistar rats (weighing 150-160 g) were fed a highly cariogenic diet, Keyes 2000 (milk powder, sugar, wheat flour, yeast, egg white powder, salt) and 10% sucrose water. Twelve weeks later, the rats were sacrificed, and their mandibular molars were removed and coated with different color developers prepared in this example (Formulations 1 to 3) for observation. A control group was treated with a commercially available color developer (trade name: Japan Ci Dental Plaque Developer, product ingredients: purified water, sodium benzoate, anhydrous ethanol, xylitol, sodium saccharin, Food Red 105).

[0084] 1.2 Preparation of liquid color developer

[0085] The formula for the liquid dental plaque developer consists of food coloring, cellulose, alcohols, potassium salts, a pH adjuster, an antibacterial agent, flavoring, and water. The food coloring is erythrosine and brilliant blue. Red pigments can stain both new and old plaque, while blue pigments only stain old plaque. Therefore, the combined dyeing can distinguish between new and old plaque. The cellulose is hydropropyl methylcellulose. The alcohol is xylitol, which is both a sweetener and a moisturizer, and has an anti-caries effect. The potassium salts are potassium citrate and potassium sorbate, which can effectively relieve tooth sensitivity symptoms. Potassium sorbate also acts as an antibacterial agent. Strawberry flavoring gives the developer a pleasant smell. The content of each component, measured in mass volume percentage, is as follows:

[0086]

[0087] 1.3 Results and Analysis

[0088] like Figure 1As shown, both the commercially available developer (left) and the liquid developer developed in this example (right, Prescription 1) exhibited darker staining in the interdental spaces, indicating a greater accumulation of plaque in these spaces. The liquid developer developed in this example exhibited superior plaque visualization. Furthermore, it can be seen that both developers exhibited strong nonspecific adsorption, and due to the high fluidity of the liquid developer, the gums were also stained.

[0089] Further check the elution property of the color developer after use. After color development with a commercially available color developer, use distilled water for washing and rinsing. Figure 2 As shown, commercially available developers have strong nonspecific adsorption, and a large amount of pigment is still deposited after washing, which brings great inconvenience to the use process and is also a disadvantage of existing developers on the market.

[0090] In the embodiment of the present application, the type and content of the pigment in the liquid developer formula were adjusted to obtain the developer of formula 2 and formula 3, which also failed to significantly improve the problem of difficult elution. Figure 3 As shown, the commercially available color developer and the liquid color developer developed in the examples of this application also exhibit non-specific adsorption to the gums, which is not conducive to subsequent elution by the user. Therefore, it is particularly important to explore new color developers.

[0091] Example 2 Preparation of thermosensitive gel developer

[0092] The establishment of the dental plaque model in this embodiment is the same as that in Example 1.

[0093] The thermosensitive gel developer provided in this embodiment comprises edible coloring, a charge protection agent, an adhesive material, a temperature-sensitive material, an antibacterial agent, and an appropriate amount of water. The edible coloring is erythrosine and brilliant blue, and the temperature-sensitive materials are poloxamer 407 and poloxamer 188. The charge protection agent, adhesive material, and antibacterial agent are also added. The charge protection agent improves the mechanical strength of the gel, and the adhesive material enhances the developer's ability to adhere to dental plaque.

[0094] Preparation: Accurately weigh erythrosine, brilliant blue, and an antibacterial agent and dissolve them in an appropriate amount of distilled water. Weigh the charge protection agent, adhesive material, and temperature-sensitive materials (poloxamer 407 and poloxamer 188) and add them to the developer solution, stirring evenly. Seal the container and incubate at 4°C overnight to fully swell.

[0095] Directions:

[0096] 1. Use a cotton swab to dip a small amount of the thermosensitive gel developer prepared in this example and evenly apply it on the tooth surface. After 5 minutes, remove the gel and rinse with mouthwash.

[0097] 2. Look in the mirror and see that the color developer attached to the teeth is dental plaque. Then use the correct brushing method to clean the displayed area.

[0098] Determination of gelation temperature:

[0099] The gelation temperature refers to the phase transition temperature when the thermosensitive gel changes from a liquid state to a semi-solid state. The gelation temperature is determined by the test tube inversion method. 3 ml of gel color developer stored at 4°C is placed in a clean and dry glass test tube, and a thermometer is inserted into the gel solution. The test tube is placed in a constant temperature water bath for 10 minutes to balance. The liquid level in the test tube must be 2 cm lower than the water bath liquid level. Set the initial temperature of the water bath to 10°C, and continue to heat up slowly at a rate of 2 minutes. For every 1°C increase in temperature, quickly tilt the test tube 90° and observe the flow of the contents. When it is observed that the contents of the test tube no longer flow and can be maintained for more than 30 seconds, the thermometer reading at this time is recorded as the gelation temperature. Take 3 samples for each prescription for measurement, measure each sample 3 times, and take the average value of the results.

[0100] Prescription screening

[0101] Poloxamer 407 (P407) and Poloxamer 188 (P188) were selected as thermosensitive gel matrices, and the mass volume fractions of P188 and P407 were used as the investigation factors (including only water, P188 and P407, with a total volume of 20 ml), and the phase transition temperature (T m ) is the response value, and the results are shown in Table 1.

[0102] Table 1 Phase transition temperature of gels obtained from different compositions of poloxamer (℃)

[0103]

[0104] It can be seen from Table 1 above that as the proportion of poloxamer 407 increases, the phase transition temperature of the thermosensitive gel decreases continuously, and as the proportion of poloxamer 188 increases, the phase transition temperature of the thermosensitive gel increases continuously.

[0105] Next, the proportion of temperature-sensitive materials in the gel to which adhesive materials and charge protectants were added was screened. As shown in Table 2, the normal human oral temperature is 36.3-37.2°C. This product is designed to undergo a phase change during oral use, thereby achieving color development of dental plaque while facilitating subsequent elution. It can be seen that Group 1 meets the requirements, and this component proportion was selected for subsequent screening.

[0106] Table 2 Blank gel prescription screening

[0107]

[0108] Next, we added the color developers erythrosine and brilliant blue to the system and screened their concentrations. As shown in Table 3, the phase transition temperatures of Group 2 and Group 4 met the requirements. Considering that preservatives are particularly important for product stability, we added the antibacterial agent sodium benzoate to the system and measured the phase transition temperature after addition. We found that the addition of the antibacterial agent did not affect the phase transition temperature.

[0109] Table 3 Screening of thermosensitive gel developer prescriptions

[0110]

[0111] Comparison of the nonspecific adsorption and elution of the thermosensitive gel prepared by adding 0.1% sodium benzoate, an antibacterial agent, to the fourth group of formulas in Table 3 and the commercially available color developer:

[0112] After applying the thermosensitive gel developer and placing it in an oral simulation environment, the thermosensitive gel developer undergoes a phase change, transforming from a liquid into a solid film that adheres to the tooth surface, thereby coloring the dental plaque. Figure 4 As shown, both colorants produced darker staining in the spaces between teeth, suggesting a greater presence of plaque deposits in these spaces. Due to the high fluidity of commercially available colorants, areas beyond the teeth were also stained. Thermosensitive gel colorants, due to their rapid phase transition, overcome the nonspecific adsorption problem caused by the high fluidity of liquid colorants. Furthermore, compared to commercially available colorants, they are easier to remove using distilled water washing, overcoming their difficulty in elution.

[0113] Example 3

[0114] The plaque thermosensitive gel developers 1 to 6 provided in this embodiment have a phase transition temperature between 31 and 33° C., are suitable for use in the oral environment, have high color development specificity, reduce nonspecific adsorption, and are easy to elute.

[0115] Dental plaque thermosensitive gel developer 1, with a phase transition temperature of 31°C, has the following components in terms of total mass percentage:

[0116] Erythrosine: 0.8%

[0117] Brilliant Blue: 0.4%

[0118] Sodium alginate: 1%

[0119] Povidone K30: 0.05%

[0120] Hydroxypropyl methylcellulose: 1.5%

[0121] Poloxamer 407: 22.5%

[0122] Poloxamer 188: 5%

[0123] Sodium benzoate: 0.1%

[0124] The balance is water.

[0125] Dental plaque thermosensitive gel developer 2, with a phase transition temperature of 31°C, has the following components in terms of total mass percentage:

[0126] Erythrosine: 0.8%

[0127] Sodium alginate: 1%

[0128] Povidone K30: 0.05%

[0129] Hydroxypropyl methylcellulose: 1.5%

[0130] Poloxamer 407: 22.5%

[0131] Poloxamer 188: 5%

[0132] Sodium benzoate: 0.1%

[0133] The balance is water.

[0134] Dental plaque thermosensitive gel developer 3, with a phase transition temperature of 31°C, has the following components in terms of total mass percentage:

[0135] Brilliant Blue: 0.4%

[0136] Sodium alginate: 1%

[0137] Povidone K30: 0.05%

[0138] Hydroxypropyl methylcellulose: 1.5%

[0139] Poloxamer 407: 22.5%

[0140] Poloxamer 188: 5%

[0141] Sodium benzoate: 0.1%

[0142] The balance is water.

[0143] Dental plaque thermosensitive gel developer 4, with a phase transition temperature of 33°C, has the following components in terms of total mass percentage:

[0144] Erythrosine: 2%

[0145] Brilliant blue: 1%

[0146] Sodium alginate: 0.8%

[0147] Povidone K30: 0.04%

[0148] Hydroxypropyl methylcellulose: 1.25%

[0149] Poloxamer 407: 18.75%

[0150] Poloxamer 188: 4.16%

[0151] Sodium benzoate: 0.1%

[0152] The balance is water.

[0153] Dental plaque thermosensitive gel developer 5, with a phase transition temperature of 33°C, has the following components in terms of total mass percentage:

[0154] Erythrosine: 2%

[0155] Sodium alginate: 0.8%

[0156] Povidone K30: 0.04%

[0157] Hydroxypropyl methylcellulose: 1.25%

[0158] Poloxamer 407: 18.75%

[0159] Poloxamer 188: 4.16%

[0160] Sodium benzoate: 0.1%

[0161] The balance is water.

[0162] Dental plaque thermosensitive gel developer 6, with a phase transition temperature of 33°C, has the following components in terms of total mass percentage:

[0163] Brilliant blue: 1%

[0164] Sodium alginate: 0.8%

[0165] Povidone K30: 0.04%

[0166] Hydroxypropyl methylcellulose: 1.25%

[0167] Poloxamer 407: 18.75%

[0168] Poloxamer 188: 4.16%

[0169] Sodium benzoate: 0.1%

[0170] The balance is water.

[0171] In the description of this specification, the reference terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" mean that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art can combine and combine different embodiments or examples described in this specification and features of different embodiments or examples without contradiction.

[0172] Although the embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and are not to be construed as limitations on the present invention. A person skilled in the art may change, modify, replace and modify the above embodiments within the scope of the present invention.

Claims

1. A thermosensitive gel developer for dental plaque, characterized in that: It contains food coloring, charge protection agent, adhesive material, temperature sensitive material, antibacterial agent and water. The content of each component is as follows in terms of mass volume percentage: Food coloring: 0.4%-5%; Charge protection agent: 0.5%-2%; Adhesive materials: 0.5%-3%; Temperature-sensitive materials: 10%-55%; and Antibacterial agent: 0.05%-0.3% The balance is water.

2. The thermosensitive plaque gel developer according to claim 1, characterized in that It contains food coloring, charge protection agent, adhesive material, temperature sensitive material, antibacterial agent and water. The content of each component is as follows in terms of mass volume percentage: Food coloring: 0.4%-3%; Charge protection agent: 0.8%-1%; Adhesive materials: 1.2%-1.6%; Temperature-sensitive materials: 20%-30%; Bacteriostatic agents: 0.05%-0.3%; and The balance is water.

3. The thermosensitive plaque gel developer according to claim 1 or 2, characterized in that: The charge protection agent is at least one of sodium alginate, sodium chloride and mannitol.

4. The thermosensitive plaque gel developer according to claim 1 or 2, characterized in that The adhesive material is at least one of povidone K30, hydroxypropyl methylcellulose, methylcellulose, and chitosan.

5. The thermosensitive plaque gel developer according to claim 1 or 2, characterized in that The antibacterial agent is at least one of sodium benzoate, potassium sorbate, ethylparaben, sorbic acid, and sodium propylparaben.

6. The thermosensitive plaque gel developer according to claim 2, characterized in that The adhesive material is povidone K30 and hydroxypropyl methylcellulose.

7. The thermosensitive plaque gel developer according to claim 3, characterized in that Measured in percentage by mass volume, the thermosensitive plaque gel developer comprises: Povidone K30: 0.03%-0.06%; and Hydroxypropyl methylcellulose: 1.2%-1.6%.

8. The thermosensitive plaque gel developer according to claim 2, characterized in that: The temperature-sensitive materials are poloxamer 407 and poloxamer 188.

9. The thermosensitive plaque gel developer according to claim 8, characterized in that Measured in percentage by mass volume, the thermosensitive plaque gel developer comprises: Poloxamer 407: 18%-23%; and Poloxamer 188: 4%-6%.

10. The thermosensitive plaque gel developer according to any one of claims 6 to 9, characterized in that The food coloring is erythrosine and brilliant blue, and the dental plaque thermosensitive gel developer comprises, by weight volume percentage, 0.8% erythrosine and 0.4% brilliant blue; or the dental plaque thermosensitive gel developer comprises 2% erythrosine and 1% brilliant blue; Optionally, the food coloring is erythrosine, and the dental plaque thermosensitive gel developer comprises, by mass volume percentage: erythrosine 0.8%-2%; Optionally, the food coloring is brilliant blue, and the plaque thermosensitive gel developer comprises, by weight volume percentage, 0.4%-1% brilliant blue.

Images