115 results about "In situ analysis" patented technology

Provided herein are methods, compositions, and systems for improved in situ detection of analytes and spatial analysis using, e.g., a sequencing readout.

The invention discloses an intelligent gradient extraction process and method for nutritional ingredients in yak bone marrow, and belongs to the technical field of bioactive substance extraction. The extraction method comprises the following four steps: gradient pressure supercritical degreasing, double-enzyme synergistic gradient enzymolysis, acid concentration gradient demineralization and temperature gradient gelatin extraction, and through the process coupling design of gradient pressure degreasing, buffer enzymolysis, graded acidolysis and membrane separation of gelatin, the high yield of the product in the previous stage is ensured; active structures of follow-up components are reserved to the greatest extent. Meanwhile, a sensing-decision-execution closed-loop system is constructed, real-time data sources such as NIR moisture sensing, online OPA detection and XRD in-situ analysis are integrated, technological parameters are dynamically adjusted through a multi-objective optimization algorithm, and global optimization of the yield, the purity and the energy consumption is achieved.

The invention belongs to the technical field of high-temperature and high-pressure free radical in-situ analysis, and provides a visual detection method for free radicals generated by hydrocarbon-water interface micro-droplets at high temperature and high pressure. The method comprises the following steps: preparation of a free radical fluorescence indicator aqueous solution, sample loading of a fused quartz capillary tube, visual simulation experiment and free radical fluorescence phenomenon analysis. On the basis of the temperature and pressure range of the actual stratum burying condition, the micro-droplet fluorescence phenomenon of the free radical fluorescence indicator water solution and the hydrocarbon liquid interface under the high-temperature and high-pressure conditions is observed through a visual fused quartz capillary thermal simulation experiment, and the fluorescence characteristics of a laser confocal microscope are used for detection. Comprehensively obtaining a method for in-situ online detection of generation of free radicals in the high-temperature and high-pressure process; the problem that micro-droplet free radicals cannot be directly detected under high-temperature and high-pressure conditions is solved.

The present invention, among other things, provides technologies for detecting and / or quantifying nucleic acids in cells, tissues, organs, or organisms. Pre-designed barcodes are associated with specific molecular targets through sequential hybridization experiments. A pseudo-color based barcoding scheme is described that overcomes the limitations in the previous generation of the technology. The current method can be applied to both in vitro and in situ analysis.

The invention relates to an in-situ analysis detection method and device for electrical contact surface film damage. The in-situ analysis detection method comprises the following steps: 1) adjusting the height and angle of a sample test platform to a proper position; 2) switching on an electric contact test platform circuit, simulating an in-situ working process of an electric contact, and obtaining electric contact information and elliptically polarized light detection information; (3) transmitting polarized light detection information to light source acquisition equipment, and (4) establishing a proper physical optical model and a vibrator model, and performing data fitting analysis to obtain the in-situ change condition of the surface of the electrical contact. By adopting a non-contact in-situ spectrum testing technology, the early-stage in-situ failure process of the electrical contact is accurately, quickly and nondestructively detected in real time, and an equipment means and a test basis are provided for research on an electrical contact early-stage failure mechanism of an electrical contact material.

The present disclosure relates to a method for in situ analysis of single nucleotide variations in an RNA sample, the method comprising the steps of: (a) contacting the RNA sample with a plurality of chimeric padlock probes comprising a modified base at the 3'end portion; (b) adding an RNA ligase to generate a cyclized padlock oligonucleotide for a padlock probe comprising a modified base complementary to the corresponding position of the RNA sample under conditions and reagents allowing the ligation of the padlock probe with single nucleotide specificity; (c) amplifying the cyclized padlock oligonucleotide under conditions and reagents that allow rolling circle amplification, thereby producing an amplified cyclized padlock oligonucleotide; and (d) detecting the amplified cyclized padlock oligonucleotide so as to obtain a map of the mononucleotide in the RNA sample. The disclosure further relates to a kit for in situ analysis of single nucleotide variations in an RNA sample.

The invention relates to the technical field of electrochemical sensors, in particular to an electrochemical gas sensor state automatic analysis method and device and a storage medium. The method comprises the following steps: acquiring degradation data corresponding to the electrochemical gas sensor through an in-situ analysis model; preprocessing the degradation data; inputting the preprocessed degradation data into a target feature prediction model for performance simulation to obtain performance prediction data; inputting the performance prediction data into a target accelerated degradation database for degradation matching to obtain evaluation data; and classifying and correcting the state of the electrochemical gas sensor according to the evaluation data. The factors influencing the performance of the electrochemical gas sensor can be quickly obtained and corrected in a complex environment.

The invention provides an argon ion beam scanning electron microscope double-beam system and an in-situ sample analysis method, an electron optical lens barrel of the system generates a high-energy electron beam for scanning the surface of a sample for imaging; the ion optical lens cone generates a high-energy argon ion beam for sample surface polishing; the sample chamber is used for accommodating samples and providing a vacuum environment; the sample table is used for accurately controlling the position and angle of a sample; and the detector is used for collecting signals generated by interaction of the electron beam and the sample for imaging analysis. According to the invention, a sample transfer step is eliminated, so that the sample preparation and analysis efficiency is greatly improved; a millimeter-scale large-size sample can be treated, and a sample with a flat and smooth surface can be obtained; sample polishing and imaging are carried out in the same vacuum environment, so that sample pollution is reduced to the greatest extent, and the accuracy of an analysis result is improved; by combining argon ion beam polishing and scanning electron microscope imaging technologies, efficient, high-precision and in-situ analysis of the sample is realized, so that more comprehensive and more accurate microstructure information is obtained.

Provided herein are methods for multiplexed in situ analysis of biomolecules in a tissue. In particular, provided herein are methods for multiplexed single-cell in situ protein and nucleic acid profiling in fixed or fresh tissues, that allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.

The invention belongs to the technical field of in-situ analysis of water environment interface nutrient elements ammonia nitrogen and nitrate nitrogen, and particularly relates to a zeolite and S110MH mixed adsorption film and a preparation method and application thereof. The zeolite and S110MH mixed adsorption film provided by the invention comprises agar hydrogel and adsorbent particles dispersed in the agar hydrogel, and the adsorbent particles comprise zeolite particles and anion exchange resin particles. The invention provides a preparation method of a zeolite and S110MH mixed adsorption film, functional parameters of the mixed adsorption film and a DGT device installed on the mixed adsorption film, and a testing method of DGT in various environmental media. Experiments show that the DGT device provided with the zeolite and S110MH mixed adsorption film can accurately test the time average concentration or diffusion flux of ammonia nitrogen and nitrate nitrogen at rhizosphere of a water body, sediment or aquatic plants, and reflect the bio-availability, geochemical process and mobility of inorganic nitrogen.

Devices, systems and methods for in-situ analysis of drilling fluid solids is provided. A system includes a digital imaging system and a computer system operatively coupled to the digital imaging system. The digital imaging system is coupled to a shale shaker. The shale shaker is positioned at a surface of the Earth adjacent a wellbore and configured to receive a drilling fluid including solid objects. The digital imaging system is configured to capture images or measurement data of the solid objects. The computer system includes processors, and a computer-readable medium storing instructions executable by the processors to perform operations. The operations include receiving the images or measurement data captured by the digital imaging system and determining one or more properties of a target solid object of the solid objects. The one or more properties include specific gravity or composition of the target solid object.

The invention relates to the technical field of geological sample preparation equipment, and particularly discloses a sample preparation device for carbonite carbon-oxygen isotope microcell in-situ analysis and a working method.The sample preparation device comprises a sample pool and a sample bin, carbonite samples are placed in the sample pool, and a plurality of supporting and adjusting electric cylinders are arranged at the bottom in the sample pool; the supporting and adjusting electric cylinder supports the bottom of the carbonate rock sample, the carbonate rock sample is wrapped with resin, the reaction micro-area is exposed, and the supporting and adjusting electric cylinder independently lifts to control the reaction micro-area to face right above; a conveying mechanism is arranged in the sample bin, a sample pool is placed on the conveying mechanism, the conveying mechanism controls the sample pool to move, a sealing cover is arranged at the upper part of the sample bin, a sealing plug and an observation window are arranged on the sealing cover, a phosphoric acid micro-injection needle penetrates through the sealing plug in a sealing manner, and a liquid outlet of the phosphoric acid micro-injection needle directly faces the reaction micro-area; the to-be-tested carbonate rock sample is wrapped by the resin, so that waste of experimental materials is avoided, the accuracy of experimental inspection is improved, and the experimental efficiency is improved.

According to the carbonate rock rare earth element content in-situ analysis method and device, carbonate rock samples are obtained, and the carbonate rock samples are from various rock forms or components; carrying out sample pretreatment on the carbonate rock sample to obtain a to-be-analyzed sample; detecting the calcium element content and the magnesium element content of the sample to be analyzed; detecting the contents of main trace elements and rare earth elements of a single carbonate mineral on the to-be-analyzed sample; and by taking the calcium element content as an internal standard, carrying out data processing on the main trace and rare earth element content of the single carbonate mineral to obtain the rare earth and yttrium element content of the single carbonate mineral. Therefore, the accurate original deposition signal of the carbonate rock sample is obtained, and the accuracy of the environmental condition and the geological history when the reconstructed sediment is formed is improved.

The invention belongs to the field of basic research of sandstone type uranium ore associated resource evaluation, and particularly relates to a method for judging the existence form of associated gallium in a sandstone type uranium deposit, which comprises the following steps of: measuring the contents of major elements and trace elements of a plurality of uranium ore samples, and judging whether gallium meets the lowest grade requirement of associated resources of the uranium deposit; for uranium ore samples exceeding the lowest grade requirement of gallium associated resources, correlation analysis of gallium elements, main elements and trace elements is carried out, and possible key mineral types closely related to gallium are preliminarily speculated; carrying out slice analysis, and circling an approximate range of speculated possible key mineral types; carrying out scanning electron microscope and energy spectrum analysis combined observation, and determining the existence form of gallium in the uranium deposit; the position exceeding the associated grade gallium is determined through laser in-situ analysis; the gallium-related mineral type is directly determined through electron probe analysis; and comprehensively judging the existence form of gallium in the uranium deposit. According to the method, the existence form of associated gallium in the sandstone type uranium deposit can be accurately and quickly judged.

The present disclosure generally relates to methods and compositions for interrogating and / or analyzing DNA methylation in a biological sample. In some aspects, the present disclosure relates to methods for determining the methylation status of a region of interest of genomic DNA. In some aspects, the methylation status is analyzed by interrogating converted DNA in which the sequence of the converted DNA is indicative of the methylation state of the DNA. In some aspects, the methods comprise generating a collective signal that is based on the methylation states of a plurality of sequences or residues in the DNA, and that is representative of the methylation status of the region of interest as a whole.

The invention relates to the field of thermochemical in-situ reaction, and discloses a thermochemical reaction tank suitable for X-ray transmission in-situ analysis, which comprises a reaction generation module and a base support module, the reaction generation module comprises a reaction tube, a thermocouple, a heating resistance wire and a temperature control device. According to the invention, X-ray transmission-type analysis can be realized, and related structural characteristic changes of the material in a working process in a high-temperature and high-pressure environment can be accurately obtained; the device has a high altitude level time resolution, and has advantages for capturing an intermediate process highly dependent on time in a reaction process; the reaction tank is not limited by reaction gas selection and has a large-temperature-range heating function, so that the reaction tank can meet complex in-situ reaction experiment conditions; the method is high in characterization expansibility, can be combined with a mass spectrometer or a spatial resolution capillary sampling mass spectrometer for use, and simultaneously monitors product dynamic information in a reaction process; the device is small in size, easy to disassemble and convenient to replace to different instrument platforms for working; the device is simple in overall structure, low in manufacturing cost and beneficial to application and popularization.

This application provides an iron-based thin-film probe, an in-situ analysis method for soil micro-interface pollutants, a screening method for iron-based materials for soil remediation, and a soil remediation method, belonging to the field of environmental analysis and testing technology. The iron-based thin-film probe, used to study the role of iron-containing mineral micro-interface pollutants in soil, is prepared from an iron sheet through in-situ surface modification; wherein the modified surface formed by the in-situ surface modification is selected from any one of nano-zero-valent iron, iron oxide, and iron sulfide; the iron-based thin-film probe is used to simulate the adsorption, distribution, and redox reactions of iron-containing mineral micro-interface pollutants in soil.

A wearable smart ring includes an annular body sized to receive a finger, photoplethysmography (PPG) sensors arranged along an inner peripheral surface, and a microfluidic perspiration collector disposed at the inner surface. The collector has microchannels that draw perspiration by capillary action into one or more reservoirs and includes a region that concentrates or retains biomarkers. A docking station mechanically receives the ring for charging and interfaces with the collector to transfer fluid and / or perform in-situ analysis. The dock includes optical instrumentation (e.g., photometry, spectroscopy such as Raman) and may implement pumping and flushing to reset the collector for subsequent use. A controller communicates results by wireless links. The system enables passive collection of perspiration during wear and detailed biomarker analysis during charging, thereby augmenting PPG-derived physiological measurements with perspiration chemistry without burdening ring power or size.

An automated distillation system includes a distillation process under vacuum, a sampling device for extracting a vapor sample of distillate, a gas analyzer for measuring a property of the vapor sample, and a controller for analyzing the property and appropriately adjusting one or more parameters of the system, such as a level of vacuum, in response to the analysis.

The present invention relates to a computer-implemented method for training a neural network for classifying images of objects suspended in a substance, preferably crystals of that substance, and to a computer-implemented method that uses this trained neural network for analyzing objects suspended in said substance, particularly crystals suspended in said substance, especially for real-time monitoring of the progress of a crystallization process of that substance. Figure 7 to be published

The application discloses a heating transmission device suitable for in-situ detection of ultrahigh-temperature pyrolysis reaction, comprising a heating pipe and an adjusting assembly; a sample outlet end of the heating pipe is connectable to a detection device; the heating pipe is divided into a low-temperature zone, a high-temperature zone and an annealing zone; a first water-cooled electrode is arranged outside the low-temperature zone; and a second water-cooled electrode is arranged outside the annealing zone; the adjusting assembly comprises a guide rail and a sliding block; and the sliding block is connected with the first water-cooled electrode or not. The application forms a variable-diameter and variable-temperature zone through combination design of the heating pipe and the water-cooled electrode, guarantees contact between the heating pipe and the protective electrode, builds a relatively slow and uniform temperature gradient in the annealing zone, guarantees generation and dispersion of product molecules and improves transmission efficiency of macromolecular compounds. The application is convenient to connect with a mass spectrometer, can realize in-situ analysis of ultrahigh-temperature pyrolysis reaction and solves the problem that macromolecular products such as polycyclic aromatic hydrocarbons, fullerenes and the like cannot be detected in-situ due to aggregation caused by intermolecular forces in the pyrolysis reaction.

The invention discloses a Q620-grade high-toughness and high-corrosion-resistance steel plate and a production method thereof. The steel plate comprises the following chemical components in percentage by weight: 0.08 to 0.09 percent of C, 0.15 to 0.40 percent of Si, 1.35 to 1.45 percent of Mn, less than or equal to 0.020 percent of P, less than or equal to 0.003 percent of S, less than or equal to 0.005 percent of Nb, less than or equal to 0.005 percent of Ti, 0.08 to 0.10 percent of Als, 0.30 to 0.70 percent of Cu, 0.7 to 1.5 percent of Ni, 0.04 to 0.05 percent of V, 0.40 to 0.45 percent of Mo, 0.30 to 0.35 percent of Cr, 0.001 to 0.002 percent of B and 0.08 to 0.30 percent of Sb. The core performance and the fatigue performance are improved by adopting a Nb-free and Ti-free design technology, the process parameters are improved through an in-situ analysis technology, the core quality of the steel plate is improved through the specific design of chemical components, and the statistical segregation degree of C, Mn, Ni, Cu and Sb is smaller than or equal to 0.05. The method is suitable for producing the steel plate with the thickness specification being smaller than or equal to 100 mm, the yield strength being larger than or equal to 620 MPa, the tensile strength being larger than or equal to 720 MPa and the impact toughness KV2 being larger than or equal to 120 J at the low temperature

The present disclosure relates in some aspects to methods for analyzing antigen receptor transcripts in a biological sample. In some aspects, nucleic acid molecules are generated from V(D)J transcripts in situ in the biological sample to enrich molecules comprising V(D)J joins. In some aspects, the presence, amount, and / or identity of a plurality of V(D)J transcripts are analyzed in situ. Also provided are oligonucleotides, sets of oligonucleotides, compositions, and kits for use in accordance with the methods.

The utility model discloses an in-situ CT (Computed Tomography) analysis device for real-time observation of a soldering tin melting process. The in-situ CT analysis device comprises a heating box, an X-ray receiving device arranged above the heating box and an X-ray emitting device arranged below the heating box, the heating box comprises a mounting box body; a first mounting groove is formed in the top of the mounting box body, and a product clamping mechanism is further mounted in the first mounting groove; and the product clamping mechanism comprises two clamping rods. Based on the X-ray detection principle, the internal structure change in the soldering tin melting process can be observed in real time, the hysteresis and destructive problems of a traditional method are solved, the annular infrared heating pipe is adopted, it can be ensured that a soldering tin sample is evenly heated, temperature change in the real welding environment is simulated, meanwhile, shielding of ray imaging is avoided, and the welding quality is improved. And the resolution and the precision of CT scanning are improved.

The present disclosure in some aspects relates to methods for assessing multi-modal in situ transcriptomics by combining microscopy and sequencing technologies and methods. In some aspects, the methods provided herein involve analyzing one or more analyte panels in a sample using a first imaging modality or set of imaging modalities. Based on said analysis, a different analyte panel and / or one or more different imaging modalities can be selected for subsequent analysis.

The invention relates to the technical field of nano material processing, and discloses an in-situ cleaning method for a transmission electron microscope sample material surface pollution layer. According to the in-situ cleaning method provided by the invention, in a high-vacuum environment of a transmission electron microscope, a controllable and localized physical and mechanical action is applied to a preselected microscopic area on the surface of a sample by using a precisely controllable nano needle tip, so that surface pollutants are directly removed in manners of scraping, stripping and the like; the nondestructive detection capability of the transmission electron microscope is used as real-time feedback, and finally, on the premise that the intrinsic structure of the sample is not damaged, the atomic-scale clean surface of the sample is exposed, and real-time in-situ analysis is carried out; compared with a traditional external preparation or in-situ cleaning technology, the method has the advantages that a pollution layer is removed by adopting a controllable mechanical contact effect, so that additional damages such as ion implantation, thermotropic degeneration and chemical pollution are fundamentally avoided, and an intrinsic structure of a material is really revealed on an atomic scale; and in addition, in-situ physical property measurement can be immediately carried out on the clean surface after cleaning.

Provided herein are methods for multiplexed in situ analysis of biomolecules in a tissue. In particular, provided herein are methods for multiplexed single-cell in situ protein and nucleic acid profiling in fixed or fresh tissues, and also allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.

The present invention discloses a multifunctional in-situ reaction device, reaction system and application. The reaction device includes a reaction vessel and a pressure maintainer. The pressure maintainer includes a main body and a cover body that are detachably connected. The main body accommodates the reaction vessel therein and forms a blank window that exposes the reaction vessel for spectral penetration. The reaction device and system of the present invention have a simple, compact and ingenious structure. The pressure maintainer accommodates and locks the reaction vessel therein. The adjusting piece and the balancing piece respectively arranged at the top and bottom of the pressure maintainer can not only protect the reaction vessel, but also eliminate the interference caused by the thermal expansion and contraction of the reaction vessel that may occur during the reaction, ensuring the accuracy of the experimental data obtained; it is not only applicable to neutron spectroscopy analysis, but also applicable to in-situ analysis of Fourier transform infrared spectroscopy and ultraviolet-visible spectroscopy, providing a multi-spectral analysis method for understanding and exploring the kinetic / thermodynamic processes and mechanisms of hydrothermal method and molten salt method.

The present disclosure relates in some aspects to methods and compositions for in situ analysis involving preparation and treatment (e.g., de-crosslinking) of biological samples. Preparation of biological samples comprising nucleic acid analytes and protein analytes for detection are provided. Methods, in which the sample with bound probes is contacted with a buffer at a temperature above 80°C, improve the detection of optical signals associated with analytes in a fixed biological sample.

The invention discloses a high and cold pasturing area greenhouse gas automatic acquisition and analysis static box, the static box comprises a box cover and a base which are connected through a sealing mechanism and form an airtight space, and an in-situ multi-gas analysis module, a multi-parameter environment sensor, a control and edge calculation unit and a wireless transmission module are integrated in the box; according to the invention, the high-precision multi-gas in-situ analysis module, the distributed environmental factor sensing network and the edge calculation unit are highly integrated in the box body, so that the system can synchronously collect key environmental parameters such as gas concentration data and soil temperature and humidity at high frequency during each automatic closed measurement period; flux calculation and data standardization processing are completed at an equipment end in real time, and finally, a result is remotely transmitted through a wireless network, so that the requirements of manual on-site sampling, air bag storage and transportation and laboratory off-line analysis are eliminated.