211 results about "In situ analysis" patented technology

A method of in situ analysis of a biological sample comprising the steps of (a) staining the biological sample with N stains of which a first stain is selected from the group consisting of a first immunohistochemical stain, a first histological stain and a first DNA ploidy stain, and a second stain is selected from the group consisting of a second immunohistochemical stain, a second histological stain and a second DNA ploidy stain, with provisions that N is an integer greater than three and further that (i) if the first stain is the first immunohistochemical stain then the second stain is either the second histological stain or the second DNA ploidy stain; (ii) if the first stain is the first histological stain then the second stain is either the second immunohistochemical stain or the second DNA ploidy stain; whereas (iii) if the first stain is the first DNA ploidy stain then the second stain is either the second immunohistochemical stain or the second histological stain; and (b) using a spectral data collection device for collecting spectral data from the biological sample, the spectral data collection device and the N stains are selected such that a spectral component associated with each of the N stains is collectable.

A method and apparatus for puncturing a surface for extraction, in situ monitoring, and/or substance delivery uses microneedles with improved properties. Applications include easy to handle glucose monitoring using a group of hollow out-of-plane silicon microneedles to sample substances in interstitial fluid from the epidermal skin layer.

Briefly described, one embodiment of the system, among others, can be implemented as follows. The system includes a computer control system and an environmental light source that is controlled by the computer control system such that an amount of light provided by the environmental light source is adjusted by the computer control so that ocular parameters of an examinee are within a targeted range. Further, the system includes an irradiation system that provides multiple angle and axial eccentricity illuminations and selective wavelength irradiation based upon instructions received from the computer control system, wherein the computer control system instructs the irradiation system to provide different irradiation characteristics for different screening procedures. Also, the system includes an image detection system that captures ocular images of the examinee, wherein the computer control system analyzes captured images and provides results of in-situ analysis. Other systems and methods are also provided.

Briefly described, one embodiment of the system, among others, can be implemented as follows. The system includes a computer control system and an environmental light source that is controlled by the computer control system such that an amount of light provided by the environmental light source is adjusted by the computer control so that ocular parameters of an examinee are within a targeted range. Further, the system includes an irradiation system that provides multiple angle and axial eccentricity illuminations and selective wavelength irradiation based upon instructions received from the computer control system, wherein the computer control system instructs the irradiation system to provide different irradiation characteristics for different screening procedures. Also, the system includes an image detection system that captures ocular images of the examinee, wherein the computer control system analyzes captured images and provides results of in-situ analysis. The system can further include a device, for example a lens or lens system, positioned in the system to be between the eye of the examinee and the image detection system during photoscreening for shifting the neutralization of the system to a desired region for a specific population of examinees. Other systems and methods are also provided.

The embodiment of the invention provides a diagenesis simulation experiment device, which contains a control device, a reaction solution supply device, a multistage continuous flow reaction device, a solution composition in situ analysis device and a core permeability evolution online detection device. The control device is respectively connected with the reaction solution supply device, the multistage continuous flow reaction device and the core permeability evolution online detection device. The reaction solution supply device is respectively connected with the multistage continuous flow reaction device and the core permeability evolution online detection device. The multistage continuous flow reaction device is connected with the solution composition in situ analysis device. The core permeability evolution online detection device is respectively connected with the multistage continuous flow reaction device and the reaction solution supply device. The diagenesis simulation experiment device provided by the invention can be used to finish more than two stages of water-rock reaction processes at different temperatures and pressures and under the condition of maintaining continuous flow of a fluid and realize continuous multistage water-rock reaction experimental simulation.

The invention discloses a continuous gas in-tube solid phase microextraction device combined with gas chromatography (GC) for use. The continuous gas in-tube solid phase microextraction device comprises a continuous gas in-tube solid phase microextraction device and a sampling device, wherein the continuous gas in-tube solid phase microextraction device is composed of an in-tube solid phase microextraction needle, a semiconductor condenser, a microheater and an inert gas connecting pipe, and the sampling device is composed of the in-tube solid phase microextraction needle, and a stop valve, a carrier gas flow controller and a gas source which are connected in sequence through a connector connected with a port of an extraction needle on one end of the in-tube solid phase microextraction needle and are used with the gas chromatography. According to the invention, the extraction needle performs thermal desorption at a GC sample inlet without changing the GC sample inlet and thus the continuous gas in-tube solid phase microextraction device is applied to the GC with any brand. The invention has the advantages of simple operating method, high extraction rate, accurate quantification, hydrophilic environment, convenient automatic operation and the like and also provides a sample pretreatment method integrated with extraction, separation and concentration. The continuous gas in-tube solid phase microextraction device can greatly promote the combination use of the SPME (solid phase microextraction) technology and the GC/MS (mass spectra) technology and applications of micro constituents in complex samples in aspects of on-site analysis, on-line analysis and in-situ analysis.

A sensor system detects organophosphorus pesticide residue by inducing the aggregation of gold nanoparticles. A method comprises aggregating gold nanoparticles by a reaction between an organophosphorus pesticide and imidazole or a green fluorescent protein (GFP), and detecting the organophosphorus pesticide based on a absorption spectral change resulting from the aggregation. The system for detecting pesticide residue is useful as a biosensor for analyzing pesticide residue in situ, because the optical change of the reagent by the presence of an organophosphorus pesticide is distinct, the detection speed is fast, and the range of detection limits is broad.

Methods and systems for measuring nuclear magnetic resonance characteristics of formation fluid utilizing micro-NMR sensors are provided. The micro-NMR sensors can be used to analyze fluid flowing through the wellbore on a periodic, continuous, and/or batch-mode basis. More efficient sampling and analysis can be conducted using the micro-NMR sensors. In situ analysis and time-lapse logging are also enabled.

The present invention, among other things, provides technologies for detecting and/or quantifying nucleic acids in cells, tissues, organs or organisms. Pre-designed barcodes are associated specific molecular targets through sequential hybridization experiments. A pseudo-color based barcoding scheme is developed to overcome limitations in the previous generation of the technology such as lack of visual signals that can be associated with the probes or small internal within cell when carrying out in situ experiments. The current method can be applied to both in vitro and in situ analysis. According to the method, each barcoding round comprises multiple serial hybridizations where a small number of colored signals (that are associated with probes) are used in each hybridization experiment within a serial hybridization round. Images from each serial hybridization experiment within the same serial hybridization round are combined to form a composite image for each barcoding round. In each barcoding round, the same set of molecular targets are analyzed. After all barcoding rounds are completed, associated of the barcode with these molecular targets is completed.

The invention relates to an in-situ detection device and an in-situ detection method for the heat conversion reaction process of a solid fuel. The detection method comprises the following steps of: putting the solid fuel into a miniature heat conversion reactor; heating the solid fuel by energizing metal, so that the solid fuel is subjected to a heat conversion reaction to generate a high-temperature gaseous product; ionizing the high-temperature gaseous product under the action of an ion source; putting the ionized high-temperature gaseous product into a mass spectrum analyzer at the other end of the miniature heat conversion reactor; and analyzing ingredients of the high-temperature gaseous product in real time, and monitoring the form variation of the solid fuel in real time by using a high-speed camera erected on the top or the lateral surface of the miniature reactor to complete multidirectional in-situ analysis on the solid fuel in the heat conversion process. The in-situ detection device and the in-situ detection method have the advantages that: quick pyrogenic decomposition can be realized, quickly-pyrolytic volatile ingredients can be analyzed in real time, and the real-time change of the form of the solid fuel can be monitored in real time.

The invention discloses a micro-flow control chip for cell tissue culture and real-time monitoring and a use method thereof. The chip comprises a glass substrate layer and a PDMS micro-flow channel layer positioned on the glass substrate layer; the glass substrate layer comprises a glass substrate and a plurality of pairs of microelectrodes arranged on the glass substrate layer; the PDMS micro-flow channel layer comprises a plurality of independent micro-fluidic channels; the microelectrodes on the glass substrate are corresponding to the micro-flow channels on the PDMS micro-flow channel layer one by one; the microelectrodes are electrically connected with an external circuit. The use method comprises the steps of cell capture, cell tissue culture, electrical impedance spectroscopy detection and tissue release. The chip disclosed by the invention is processed by a transparent substrate, microscopic imaging can be taken as a supplementary means of the electrical impedance spectroscopydetection, and the dynamic growth and the physiological behavior of cell tissues and other biological processes are subjected to observation and in-situ analysis. The micro-flow control chip can be used in the fields of biological research, drug screening and the like of cells or tissues.

The invention discloses a method for rapidly testing casting blank segregation by utilizing a direct-reading spectrometer, which is suitable for the quantitative and trending detection in the casting blank segregation, belonging to the technical field of metallurgy analyzing and detecting. In the method, the direct-reading spectrometer is adopted to simulate an in-situ analyzer to detect the casting blank segregation; and the direct-reading spectrometer segments and dots a test sample, and simulates the in-situ analyzer to analyze the test sample, so as to quantitatively detect the continuously cast blank macrosegregation. The method can overcome the defects that the in-situ analysis sample preparation is complex, the testing period is long, the detection cost is high and the like, satisfies the generalized quantitative detection to the casting blank in the continuous casting production, and provides a good popularization and reference method for the iron and steel enterprises owning rapid spectrum analyzers in the trending and quantitative detection of the continuously cast blank segregation. Compared with the traditional pickling method for detecting the casting blank segregation, the method has the same trend on the description of the casting blank segregation as that of the pickling method; the casting blank segregation result detected by the direct-reading spectrometer not only can describe the casting blank segregation condition, simultaneously can clearly describe the component segregation curve of each element, and has the visual digitized function.

The invention discloses a hierarchical pore molecular sieve N-Meso-ZSM-5 catalyst, and a method for preparing bio-oil through catalytic pyrolysis of lignin by the catalyst. The metal modified hierarchical pore molecular sieve N-Meso-ZSM-5 catalyst is obtained through modifying an H-type hierarchical pore molecular sieve N-Meso-ZSM-5 catalyst with metal, and the metal can be one or more of Ga, Ce,Ni, Fe, Zn and Co. The catalyst disclosed in the invention is obtained through isopyknic impregnation process modification of the one or more transition metals. The non-in situ analysis method for thecatalytic pyrolysis of lignin by the modified catalyst comprises the following steps: introducing the modified hierarchical pore molecular sieve catalyst into a fixed bed reactor, performing ectopiccatalytic pyrolysis on the lignin to prepare a functional chemical substance, and performing catalytic conversion separation and purification to obtain a liqid product mainly comprising phenol, guaiacol, hydroquinone and catechol.

The invention relates to a potential type micro electrode sensor, in particular to a potential type micro electrode sensor capable of being used for ion detection in settlings and application thereof.The potential type micro electrode sensor is a polymer film ion selective micro electrode, and consists of a gold micro electrode, a PEDOT (PSS) conduction layer and an iron selective polymer film; the iron selective polymer film is prepared by mixing ion selective carriers containing ions to be detected and lipophilic ion exchangers (quadri(3,5-bis(trichloromethyl)phenyl)sodium borate accordingto the mol ratio of (1:2)-(1:4) and then adding polyvinyl chloride and o-nitrophenyl octyl ether. The solid contact type micro electrode has the advantages that the manufacturing is simple and convenient; the sensitivity is high; the cost is low; the miniaturization is easy, and the like. Meanwhile, the micro electrode mass transfer speed is high; the current density is great; the response speed is high, and the like. The ion field in-site analysis in settlings can be realized; the detection on various ions in the settings can be realized through changing the types of ion carriers in the ion selective films.

A gas separation and detection tool for performing in situ analysis of borehole fluid is described. A separation system such as a membrane is employed to separate one or more target gasses from the borehole fluid. The separated gas may be detected by reaction with another material or spectroscopy. When spectroscopy is employed, a test chamber defined by a housing is used to hold the gas undergoing test. Various techniques may be employed to protect the gas separation system from damage due to pressure differential. For example, a separation membrane may be integrated with layers that provide strength and rigidity. The integrated membrane separation may include one or more of a water impermeable layer, gas selective layer, inorganic base layer and metal support layer. The gas selective layer itself can also function as a water impermeable layer. The metal support layer enhances resistance to differential pressure. Alternatively, the chamber may be filled with a liquid or solid material.

The present invention is in-site analysis method of propylene ammoxidizing product and aims at providing automatic relatively simple and time-saving in-site analysis method of propylene ammoxidizing product. The technological scheme of the present invention adopts twice direct gas samplings, including the first to complete the separation and rationing ammonia component and the second to complete the rationing of acrylonitrile, hydrocyanic acid, acraldehyde, acetonitrile and acrylic acid components. The present invention may be used in the industrial production of acrylonitrile.

The present invention is ultraviolet oxidation process and device for intermittent in-situ quick resolution of total phosphorus and total nitrogen. Into measured water sample, supersulfate in 1-30 % of solution weight as slaking agent is added, and ultraviolet light source and stirrer are turned on to slake components in water under constant temperature in 15-70 deg.c and normal pressure. The device for the technological process includes heater, stirrer, temperature detector, ultraviolet light source and slaking unit. The present invention can result in slaking rate of total phosphorus and total nitrogen up to 90 %, meeting the requirement for in-situ analysis.

The invention discloses a device for analyzing chemical components of a single suspension particle, comprising a pulse laser, and further comprising a hollow beam particle capturing system, an atomicemission spectrum collecting system, a Raman spectrum collecting system and an imaging system. The hollow beam particle capturing system comprises a continuous laser, a hollow beam generating apparatus, an expanded-beam collimating apparatus, a high-reflecting mirror, a first converging lens and a sample pool. The sample particles are arranged in the sample pool. The atomic emission spectrum collecting system comprises a first coupling lens and a laser induced breakdown spectrometer. The Raman spectrum collecting system comprises a second coupling lens and a Raman spectrometer. In addition, the invention further provides a method for analyzing the chemical components of the single suspension particle by adopting the device. The method for analyzing chemical components of single suspensionparticle captures the sample particles by the hollow light beam and ionizes the sample particles with the pulse laser, collects the atomic emission spectrum information and the Raman spectrum information of the sample particles, and realizes the in-situ analysis of the elemental composition and the material components of the single suspended particle.