Micro-flow control chip for cell tissue culture and real-time monitoring and use method thereof

Abstract

The invention discloses a micro-flow control chip for cell tissue culture and real-time monitoring and a use method thereof. The chip comprises a glass substrate layer and a PDMS micro-flow channel layer positioned on the glass substrate layer; the glass substrate layer comprises a glass substrate and a plurality of pairs of microelectrodes arranged on the glass substrate layer; the PDMS micro-flow channel layer comprises a plurality of independent micro-fluidic channels; the microelectrodes on the glass substrate are corresponding to the micro-flow channels on the PDMS micro-flow channel layer one by one; the microelectrodes are electrically connected with an external circuit. The use method comprises the steps of cell capture, cell tissue culture, electrical impedance spectroscopy detection and tissue release. The chip disclosed by the invention is processed by a transparent substrate, microscopic imaging can be taken as a supplementary means of the electrical impedance spectroscopydetection, and the dynamic growth and the physiological behavior of cell tissues and other biological processes are subjected to observation and in-situ analysis. The micro-flow control chip can be used in the fields of biological research, drug screening and the like of cells or tissues.

Description

technical field [0001] The invention relates to microfluidic technology and a biological cell tissue culture device, in particular to a microfluidic chip for cell tissue culture and real-time monitoring and its application method. Background technique [0002] Before the official application of new drugs, animal experiments and human clinical trials are generally required. Due to the huge difference between animals and humans, the information provided in animal experiments is unreliable, and animal experiments involve ethical issues, while human clinical trials will bring potential harm to the bodies of experimental volunteers due to unstable factors such as side effects of drugs. On the other hand, there are huge uncertainties and risks of failure. Therefore, the dynamic culture of in vitro biological tissues and drug experiments are important means to solve the problems of long drug development cycle and high cost. [0003] The in vitro culture of cells is an important t...

AI Technical Summary

Problems solved by technology

[0006] The development and application of microfluidic tissue chips currently used for tissue culture or drug testing are limited in the following aspects: the lack of effective means to precisely manipulate cells and grow them into three-dimensional spherical tissues; the current real-time Detection is generally achieved through microscopic imaging, and phototoxicity has a certain impact on the physiological state of cells and tissues; it is difficult to perform high-throughput experiments, and the sample data is small, lacking statistical significance; tissue samples are not easy to take out for subsequent research and experiments

Images