The invention provides a method for detecting
lysozyme. The method comprises the following steps of dispensing a
metal organic skeleton solution to the surface of a gold
electrode, carrying out incubating, washing and
nitrogen drying; dispensing a
lysozyme aptamer solution to the surface of the gold
electrode, carrying out incubating, washing and
nitrogen drying and carrying out first electrochemical impedance
spectroscopy detection; washing the gold
electrode after first electrochemical impedance
spectroscopy detection, carrying out
nitrogen drying, dispensing the solution to a to-be-detected sample, carrying out incubating, washing and nitrogen drying, and carrying out second electrochemical impedance
spectroscopy detection; subtracting
electron transfer resistance in a first
electrical impedance spectroscopy from the
electron transfer resistance in a impedance spectroscopy of the to-be-detected sample to obtain an
electron transfer resistance difference and comparing with a default
standard curve to obtain the concentration of the
lysozyme. According to the embodiment of the invention, the adopted detection method is simple in operation, marking is not needed, the flexibility is relatively high, the
detection limit can reach 2.0*10<-11>mol / L and the
linear range is 1.0*10<-7>mol / L to 1.0*10<-11>mol / L.