Pyrethroid insecticide degradation bacteria and method for preparing fungicide thereof

A technology for pyrethroids and pesticides, which is applied in the field of pyrethroid pesticide-degrading bacteria, and can solve the problems that the degrading bacteria and the preparation method thereof have not yet been found.

Inactive Publication Date: 2010-06-09
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, no literature has been found to isolate the degrading bacteria of pyrethroid pesticides from the phyllosphere of plants and the preparation method of their bacterial agents.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: preparation of pyrethroid degrading bacterial agent

[0023] 1) Medium preparation: a) LB medium (solid, 1L): peptone 10g, yeast extract 5g, sodium chloride 5g, agar 20g, add water to 1L, pH 7.0; b) LB medium (liquid, 1L): Peptone 10g, yeast extract 5g, sodium chloride 5g, add water to 1L, pH 7.0; c) seed medium (liquid, 1L): MgSO 4 0.10g, KH 2 PO 4 0.50g, NaCl 0.50g, K 2 HPO 4 1.50g, 10g sucrose, 5g peptone, add water to 1L, pH 7.0;

[0024] d) Fermentation medium (liquid, 1L): MgSO 4 0.10g, KH 2 PO 4 0.50g, NaCl 0.50g, K 2 HPO 4 1.50g, 10g sucrose, 5g corn steep liquor, add water to 1L, pH 7.0; all the above media were sterilized at 121°C for 20min.

[0025] 2) Strain activation: pick pyrethroid-degrading bacteria to LB solid medium, pick a single colony by continuous streaking at 28°C for two times, pick a single colony in LB liquid medium, and shake at 28°C, 150r / min shake culture culture overnight;

[0026] 3) Preparation of liquid seeds: ...

Embodiment 2

[0029] Example 2: Degradation effect of pyrethroid degrading bacteria agent on beta-cypermethrin on leaves of Euonymus euphratica

[0030] 1) Select Euonymus Euonymus without the application of beta-cypermethrin, select two plots, each 15m 2 , the spraying rate is 3g / mu;

[0031] 2) After 6 hours, evenly spray 100mL of pesticide-degrading bacteria 9 cfu / mL), with spraying water as the control;

[0032] 3) After 2 days, the five-point sampling method was used to detect the residue of beta-cypermethrin on the leaves of Euonymus euphratica, and it was found that the residue of beta-cypermethrin in the treatment group was reduced by 76% compared with the control group.

Embodiment 3

[0033] Example 3: Degradation Effect of Pyrethroid Degrading Bacteria Agent on Rapeseed Leaf Cyfluthrin

[0034] 4) Select the rape that has not been applied with cyfluthrin, and select two plots, each 15m 2 , the spraying rate is 1g / mu;

[0035] 5) After 6 hours, evenly spray 100mL of pesticide-degrading bacteria 9 cfu / mL), with spraying water as the control;

[0036] 6) After 2 days, the five-point sampling method was used to detect the residue of cyfluthrin on rapeseed leaves, and it was found that the residue of cyfluthrin in the treatment group was reduced by 83% compared with the control group.

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Abstract

The invention relates to a pyrethroid insecticide degradation bacteria separated from Euonymus japonica phyllosphere and a method for preparing a fungicide thereof, belonging to the biotechnology field. By identification, the bacillus is Pseudomonas aeruginosa and is preserved in CCNCC with the preservation number of CGMCC No.2699. The method for producing the fungicide comprises five steps of preparing culture medium, activating strains, preparing liquid seeds, fermenting in a liquid state and preparing the fungicide.The pyrethroid insecticide degradation fungicide produced by the technical scheme of the invention can effectively degrade the pyrethroid insecticides on plant leave surfaces, reduce insecticide residual, ensure food safety and protect ecological environment.

Description

technical field [0001] The invention relates to a pyrethroid pesticide-degrading bacterium isolated from the phyllosphere of Euonymus euphratica and a preparation method for the bacterium agent. Belongs to the field of biotechnology. technical background [0002] Pyrethroid pesticides are broad-spectrum insecticides with quick-acting, high-efficiency, low toxicity, low residue, and safety to crops. In addition to having special effects on more than 140 kinds of pests, some pyrethroid pesticides are also effective on underground pests and Mite pests have a better control effect. Chinese patent (authorized announcement number: CN 1063017C) discloses a compound insecticide with Aifodin and pyrethroid (fenvalerate, cypermethrin) as active ingredients, mainly used to control diamondback moth, Spodoptera litura, etc. Agricultural pests. Chinese patent (authorization announcement number: CN 1242675C) has announced a kind of with abamectin and beta-cypermethrin as main component,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02A62D101/04C12R1/385
Inventor 白志辉谷立坤唐玲荆梦庄国强张洪勋
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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