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Degrading bacteria for pyrethroids insecticides and bactericide thereof

A technology for degrading pyrethroids and pesticides, which is applied in the field of biological high-tech, can solve the problem of no degrading bacterial agent of high-efficiency cypermethrin pesticides and the like, and achieves the promotion of coordination and unification of pesticides for pest control and food safety, good degradation effect and low production cost. Effect

Active Publication Date: 2011-02-16
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Beta-cypermethrin is a pyrethroid pesticide that has been widely used in recent years, and no degrading bacteria specifically targeting beta-cypermethrin pesticides have been found so far

Method used

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  • Degrading bacteria for pyrethroids insecticides and bactericide thereof
  • Degrading bacteria for pyrethroids insecticides and bactericide thereof
  • Degrading bacteria for pyrethroids insecticides and bactericide thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Preparation method and growth status determination of pyrethroid pesticide-degrading bacteria HP-S-01:

[0034] Activate the strain of pyrethroid pesticide-degrading bacteria HP-S-01 on a solid plate and inoculate it on the inclined surface of the test tube. According to the following formula, Gaoshi synthetic No. 1 medium was prepared ( Synthetic Medium No.1): Potassium nitrate 1g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.5g, sodium chloride 0.5g, ferrous sulfate 0.01g, soluble starch 20g, distilled water 1000mL. Add 0.1 g of wet bacteria to the liquid Gauchy Synthetic Medium No. 1 at pH 7.5, at 26°C, 150r min -1 Cultivate in a shaker for 1-7d.

[0035] See Table 1 for the relationship between the culture time and the growth status of the degrading bacteria HP-S-01.

[0036] Table 1 The influence of culture time on the dry weight of mycelium of pyrethroid pesticides degrading bacteria

[0037]

[0038]

[0039] Note: the above results were re...

Embodiment 2

[0041] Degradation effect of degrading bacteria HP-S-01 on lambda-cypermethrin, lambda-cyhalothrin, deltamethrin, lambda-cyhalothrin (Kung Fu):

[0042] In the basal salt medium (the components are: NH 4 NO 3 1.0g, NaCl 0.5g, K 2 HPO 4 1.5g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, water 1000mL, pH 7.5) were added to the final concentration of 50mg.L -1 Beta-cypermethrin, beta-cyfluthrin, deltamethrin, beta-cyhalothrin (Kung Fu) and other pyrethroid pesticides were inoculated with degrading bacteria HP-S-01, and the culture medium without bacteria was set as a control. After 2 days of bed cultivation, the degradation of pesticides was detected by high performance liquid chromatography, and the results are shown in Table 2. The results showed that the degrading bacteria could effectively remove residues of pyrethroid pesticides such as beta-cypermethrin, beta-cyfluthrin, deltamethrin, and beta-cyhalothrin (Kung Fu), and had a good degradation effect.

[0043] Table 2 Deg...

Embodiment 3

[0049] (1) Activate the strain of pyrethroid pesticide-degrading bacteria HP-S-01 on a solid plate, and measure the degradation performance, and inoculate it on the inclined surface of the test tube for subsequent use;

[0050] (2) The test tube was inoculated in a 1000mL shake flask containing 250mL of Gaoshi Synthetic No. 1 medium, and was shaken at a constant temperature to the logarithmic phase, ready to inoculate the seed tank;

[0051] (3) The seed tank is 100L, the feeding amount is 80L, and the medium formula is: soluble starch 2%, KNO 3 0.1%, NaCl 0.05%, K 2 HPO 4 0.05%, MgSO 4 0.05%, FeSO 4 0.001%, at 1.1Kg / cm after feeding 3 Under the pressure of 121 °C, high-pressure damp heat sterilization, after cooling to 26 °C, inoculate the above-mentioned cultured inoculum strains into 100L seed tanks according to the inoculum amount of 8% to 10%, and cultivate them until the logarithmic growth phase. The ventilation volume of sterile air is 0.45~0.60m 3 / min, the s...

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Abstract

The invention discloses degrading bacteria for pyrethroids insecticides. The degrading bacteria are HP-S-01 and are identified to be streptomyces (Streptomyces sp.), wherein the collection number of the strain is CCTCC M 2010096; and Genbank access number of 16S rDNA of the strain is HM016873. The invention discloses a method for preparing bactericide by using the degrading bacteria at the same time. The bactericide is low in cost, convenient and desirable in degradation effect, can directly used to reduce cypermethrin pesticide residues in crops by over 80 percent, solves the problem of excessive cypermethrin pesticide residue in the agricultural production, is suitable to be used in national fruit and vegetable production and export bases or places with a green food brand mark on a large scale, and has a great significance for promoting the production of green vegetables and green food.

Description

technical field [0001] The invention provides a pyrethroid pesticide degrading bacterium and its agent, belonging to the high-tech field of biology, degrading chemical pesticide residues by means of microorganisms, and suitable for the production of green and pollution-free agricultural products in modern agricultural production. Background technique [0002] Pyrethroid pesticides are a class of biomimetic insecticides popularized and applied in agriculture in the 1970s. They target nerve sodium ion channels and have the characteristics of high efficiency and low toxicity. They have been widely used in the control of agricultural pests and sanitary pests. widely used. At present, the number of varieties and the amount of its use are second only to organophosphorus pesticides, occupying the second place in the pesticide market, accounting for about 1 / 4 of the world's pesticide market, and its application area in my country has accounted for 1 / 3 of the total application area o...

Claims

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Application Information

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IPC IPC(8): A62D3/02C12N1/20A62D101/04A62D101/28C12R1/465
Inventor 胡美英钟国华陈少华杨柳林庆胜
Owner SOUTH CHINA AGRI UNIV
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