This invention provides a fully enclosed
microfluidic chip for biodetection, comprising a base plate, a
liquid storage rotor, and a top cover. The base plate has a biodetection
reaction chamber and several channels. The biodetection
reaction chamber is pre-sealed with the reaction reagents required for biodetection. The
liquid storage rotor has several cavities, with liquid outlet channels at the bottom of the cavities. Biodetection samples are added to the cavities and transferred through them. A
piston is located within each cavity. The top cover has inclined protrusions corresponding to the positions of the channels. A motor drives the
liquid storage rotor to rotate. When the
piston rod passes through the inclined protrusions of the top cover, it moves the
piston within the cavities, pushing the liquid to transfer, thus completing the biodetection of the sample within the biodetection
reaction chamber. The
microfluidic chip of this invention has a fully sealed structure, eliminating the need to open the cover again after sample addition, thus isolating the internal environment of the
chip from the external environment, eliminating the risk of
contamination, and eliminating the need for a dedicated partitioned laboratory.