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Pesticide degrading bacterium preparation as well as preparation method and application thereof

A technology for pesticide degradation and bacterial preparations, applied in biochemical equipment and methods, methods based on microorganisms, immobilized on/in organic carriers, etc. Unclear impact, incomplete application of microbial dosage forms and other issues, to achieve the effect of energy reuse, reduce the risk of affecting the environment, and easy operation

Active Publication Date: 2014-03-26
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the effects of various adjuvants in traditional pesticide formulations on microbial organisms are not clear, the preparation of traditional pesticide formulations is not fully suitable for the development of microbial formulations
[0006] Bagasse and molasses, as low-carbon, environmentally friendly and resource-rich sugar industrial wastes, have been maturely used in the fermentation production of ethanol, xylitol and other industries, but their application in pesticide-degrading bacteria preparations has not been reported

Method used

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  • Pesticide degrading bacterium preparation as well as preparation method and application thereof
  • Pesticide degrading bacterium preparation as well as preparation method and application thereof
  • Pesticide degrading bacterium preparation as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Embodiment 1 production process modified bagasse MSB

[0051] The modified bagasse MSB is prepared by chemically modifying the bagasse, and the modified bagasse after chemical modification has better water absorption and slow-release properties.

[0052] The method of described chemical modification is:

[0053] S1. Soak 10 g bagasse in a 500 mL beaker containing 20% ​​sodium hydroxide solution, stir at room temperature for 1 h, leave overnight and filter, rinse the bagasse with distilled water until the pH value is between 8-9, set aside, It is recorded as Basified Sugarcane Bagasse (BSB).

[0054] S2. Put 10.0 g of BSB in a 500 mL Erlenmeyer flask, add 200 mL of ethanol and 40 mL of epichlorohydrin (Chloroepoxy propane), and put it in a 60 °C oil bath together with a thermostat, condenser and magnetic stirrer for 24 h , the obtained bagasse is recorded as Etherified Sugarcane Bagasse (ESB).

[0055] S3. Slowly pour 40 mL of tetraethylenepentamine (TEPA) into the ...

Embodiment 2

[0057] The preparation of embodiment 2 pesticide degrading bacteria preparation

[0058] 1. Materials

[0059] (1) The bagasse used is sugar industry waste bagasse, which is mechanically crushed to a particle size of 60 mesh.

[0060] (2) The strain used is Bacillus pumilus ( Bacillus pumilus ) of the HZ-2 strain, the strain preservation unit is the China Type Culture Collection Center, the preservation unit address is Wuhan University, Wuhan City, Hubei Province, the preservation date is September 6, 2013, and the preservation number is CCTCC NO: M 2013380.

[0061] 2. The preparation steps are as follows:

[0062] The strain HZ-2 was activated on a petri dish and inoculated on the inclined surface of a test tube; the activation method was a conventional strain activation method.

[0063] S1. inoculate the single bacterium colony on the above-mentioned slant in the shake flask of LB liquid medium, shake and cultivate to the logarithmic phase to obtain the bacte...

Embodiment 3

[0066] The preparation of embodiment 3 pesticide degrading bacteria preparation

[0067] 1. Materials

[0068] (1) The bagasse used is the modified bagasse MSB prepared in Example 1.

[0069] (2) The strain used is Bacillus cereus ( Bacillus cereus ), preserved in the Laboratory of Insect Toxicology, South China Agricultural University.

[0070] 2. The preparation steps are as follows:

[0071] The strain Bacillus cereus ( Bacillus cereus ) strains were activated on a petri dish and inoculated on the inclined surface of a test tube; the activation method was a conventional strain activation method.

[0072] S1. inoculate the single bacterium colony on the above-mentioned slant in the shake flask of LB liquid medium, shake and cultivate at room temperature to the logarithmic phase to obtain the bacterial liquid; LB liquid medium is a conventional medium;

[0073] S2. Centrifuge the bacterial solution obtained in S1 at 4800r / min for 10min, remove the supernatant, ...

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Abstract

The invention discloses an application of bagasse in the preparation of a pesticide degrading bacterium preparation, and provides a pesticide degrading bacterium preparation as well as a preparation method and an application thereof. The pesticide degrading bacterium preparation comprises components as follows: (1), strains having a degrading function on pesticide, such as a Bacilluspumilus HZ-2 strain preserved in the CCTCC (China Center for Type Culture Collection) located in Wuhan University, Wuhan City, Hubei Province on September 6th, 2013, wherein the preservation number is CCTCCNO: M2013380; and (2), a microbial preparation carrier: bagasse. The pesticide degrading bacterium preparation takes bagasse as the microbial preparation carrier and takes glucose or molasses as an auxiliary material, so that the utilization rate of industrial waste is greatly increased, the cost is low, a stable carbon source is provided for microorganisms in the preparation, and the persistent effect of the preparation is guaranteed. The pesticide degrading bacterium preparation is simple to use and good in degrading effect and has good popularization and application values.

Description

technical field [0001] The invention relates to the technical field of microbial bacterial agent and pesticide degradation. More specifically, it relates to a pesticide-degrading bacteria preparation and its preparation method and application. Background technique [0002] The excessive use of pesticides has caused widespread concern around the world on issues such as food safety, crop damage, and the development of pest resistance. living environment and health. In modern agricultural production, the government and agricultural scientists are strongly advocating the adoption of Integrated Pest Management (IPM), integrated agricultural cultivation, physical, biological control and other measures, to reduce the proportion and frequency of chemical control, and to change the use of damage to the ecological environment. In exchange for the outdated model of economic development, while sustainably controlling pests and weeds, agricultural production and the economy will be ste...

Claims

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Application Information

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IPC IPC(8): C12N11/02A62D3/02C12R1/07A62D101/04A62D101/28
Inventor 钟国华刘婕蒋刚彪翁群芳肖盈
Owner SOUTH CHINA AGRI UNIV
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