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Application of silkworm excrement in aspect of serving as vector of microbial bacteria

A technology of microbial bacteria and microbial preparations, applied in the direction of immobilization on/in organic carriers, application, botany equipment and methods, etc., can solve problems such as unapplied reports, achieve no secondary pollution, and be easy to operate , low-cost effect

Active Publication Date: 2016-09-21
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is not difficult to see that silkworm excrement is a low-cost, environmentally friendly, and highly exploitable resource. At present, there are no reports on its application in pesticide-degrading bacterial preparations.

Method used

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  • Application of silkworm excrement in aspect of serving as vector of microbial bacteria
  • Application of silkworm excrement in aspect of serving as vector of microbial bacteria
  • Application of silkworm excrement in aspect of serving as vector of microbial bacteria

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Effect test

Embodiment 1

[0066] Embodiment 1 makes carbonized silkworm excrement MCS

[0067] The carbonized silkworm excrement MCS is prepared by chemical modification, and the carbonized silkworm excrement after chemical modification has sufficient pores, and has better water absorption and slow-release properties.

[0068] The method of described chemical modification is:

[0069] S1. Use a muffle furnace to fill with nitrogen and anoxic and carbonize for 5 hours, and the carbonization temperature is 700°C. After cooling, take it out, mix and grind through a 100-mesh sieve.

[0070] S2. Use 0.1mol L of silkworm excrement -1 Phosphoric acid solution treatment for 5 hours, repeated 3 times to remove ash, all filtered, then rinsed with deionized water until neutral, put in a desiccator, dried at 60°C, recorded as carbonized silkworm excrement (MCS), and stored.

[0071] Silkworm excrement after carbonization figure 1 shown.

Embodiment 2

[0072] The preparation of embodiment 2 pesticide degrading bacteria preparation

[0073] 1. Materials

[0074] (1) The silkworm excrement used is silk industry waste silkworm excrement, which is mechanically crushed to a particle size of 60 mesh.

[0075] (2) The bacterial strain used is the HZ-2 strain of Bacillus pumilus, and the strain preservation institution is the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2013380.

[0076] 2. The preparation steps are as follows:

[0077] The strain HZ-2 was activated on a petri dish and inoculated on the inclined surface of a test tube; the activation method was a conventional strain activation method.

[0078] S1. inoculate the single bacterium colony on the above-mentioned slant in the shake flask of LB liquid medium, shake and cultivate to the logarithmic phase to obtain the bacterial liquid; LB liquid medium is a conventional medium;

[0079] S2. Centrifuge the bacterial solution obtaine...

Embodiment 3

[0081] The preparation of embodiment 3 pesticide degrading bacteria preparation

[0082] 1. Materials

[0083] (1) The silkworm excrement used is the carbonized silkworm excrement MCS prepared in Example 1.

[0084] (2) The strain used is the BSF01 strain of Bacillus subtilis, and the strain preservation unit is the China Center for Type Culture Collection, and the preservation number is CCTCC NO:AB 2014103.

[0085] 2. The preparation steps are as follows:

[0086] The strain BSF01 was activated on a petri dish and inoculated on the inclined surface of a test tube; the activation method was a conventional strain activation method.

[0087] S1. inoculate the single bacterium colony on the above-mentioned slant in the shake flask of LB liquid medium, shake and cultivate at room temperature to the logarithmic phase to obtain the bacterial liquid; LB liquid medium is a conventional medium;

[0088] S2. Centrifuge the bacterial solution obtained in S1 at 4800r / min for 10min, re...

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Abstract

The invention discloses an application of silkworm excrement in an aspect of serving as a vector of microbial bacteria, in particular an application of the silkworm excrement in preparing a pesticide-degrading bacterium preparation, and also provides the pesticide-degrading bacterium preparation as well as a preparation method and an application thereof. The pesticide-degrading bacterium preparation consists of the following components: (1) a strain having a pesticide-degrading strain; and (2) a microbial preparation vector, namely the silkworm excrement. The pesticide-degrading bacterium preparation provided by the invention uses the silkworm excrement as the microbial preparation vector, so that a stable vector is provided for microorganisms in the preparation; and the vector is capable of guaranteeing the persistent effect of the preparation, and is capable of greatly improving the utilization rate of silk industrial waste and reducing production cost. The pesticide-degrading bacterium preparation disclosed by the invention is simple to use and operate and good in degrading effect, and the preparation has good values of popularization and applications.

Description

technical field [0001] The invention relates to the technical field of microbial bacterial agent and pesticide degradation. More specifically, it relates to the application of silkworm excrement as a carrier for microorganisms. Background technique [0002] my country is a large agricultural country, and the production and use of pesticides account for one-tenth of the world's total. In modern agricultural production, the government and agricultural scientists are strongly advocating the adoption of Integrated Pest Management (IPM) to change the old model of destroying the ecological environment in exchange for economic development. While sustainably controlling pests and weeds, Steadily develop agricultural production and economy. However, the introduction of new types of pesticides and the annual increase in pesticide production have revealed an unavoidable fact that chemical agents still occupy an unshakable important position in the control of crop diseases, insect pes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/02A01N25/32
CPCA01N25/32C12N11/02
Inventor 钟国华刘婕杨红霞
Owner SOUTH CHINA AGRI UNIV
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