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Use of pseudomonas diminuta in degradation of pyrethroid pesticide residue and preparation

A pyrethroid, pseudomonas technology, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve the problem of less microorganisms

Inactive Publication Date: 2009-05-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most microorganisms degrade one or a few pesticides, and there are few reports on microorganisms that can degrade multiple pyrethroid pesticides at the same time.

Method used

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  • Use of pseudomonas diminuta in degradation of pyrethroid pesticide residue and preparation
  • Use of pseudomonas diminuta in degradation of pyrethroid pesticide residue and preparation
  • Use of pseudomonas diminuta in degradation of pyrethroid pesticide residue and preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 实施例1:降解菌株的筛选与鉴定

[0039] 1.1培养基和试剂

[0040] 富集培养基:蛋白胨10g,NaCl 1.0g,KH 2 PO 4 1.0g,H 2 O 1000mL,葡萄糖1.0g,pH7.0;

[0041] 基础培养基:NH 4 NO 3 1.00g,MgSO 4 .7H 2 O 0.5g,(NH 4 ) 2 SO 4 0.5g,KH 2 PO 4 0.5g,NaCl 0.5g,K 2 HPO 4 1.5g,H 2 O 1000mL,pH7.0;

[0042] 基础培养基加入1.5%琼脂和100mg·L -1 的联苯菊酯、甲氰菊酯和氯氰菊酯即配成相应的含农药固体培养基;

[0043] 50mg·L -1 的联苯菊酯、甲氰菊酯和氯氰菊酯乳液。

[0044] 1.2菌株对联苯菊酯、甲氰菊酯和氯氰菊酯降解效能的测定

[0045] 将纯化后的单个菌株以菌量OD 415nm =0.2接种到100mL含联苯菊酯、甲氰菊酯、氯氰菊酯各100mg·L -1 的无菌液体基础培养基的250mL三角瓶中,以不接菌的培养液作对照,在30℃,180r·min -1 的恒温摇床上振荡培养3天。培养结束后,吸取2mL培养液,加入4,4,3mL的石油醚萃取3次,加入无水硫酸钠吸水,并定容至10mL,用气相色谱检测。

[0046] 气相色谱检测条件:Agilent 6890GC(G1530N / G3172A)气相色谱仪,HP-5(30mm×0.25mm×0.25μm)色谱柱,ECD检测器,检测温度280℃,柱温260℃,进样口温度280℃,柱流:1.0mL·min -1 ,分流比为1:10,载气为N 2 (99.999%),进样量为1μL。

[0047] 降解率(%)=(对照样品残留量-处理样品残留量)×100 / 对照样品残留量

[0048] 1.3 优势降解菌株选育

[0049] 1.3.1 菌株来源

[0050] 采集杭州农药厂拟除虫菊酯类农药生产车间下水道处驯化过的污泥。

[0051] 1.3.2 降解菌株的分离、纯化和筛选

[0052] 从每份污泥土样中取10g,在无菌的条件下,分别加到含联苯菊酯、甲氰菊酯、氯氰菊酯各...

Embodiment 2

[0070] 实施例2:降解条件的优化

[0071] 1 材料与方法

[0072] 1.1 培养基和试剂

[0073] 富集培养基:蛋白胨10g,NaCl 1.0g,KH 2 PO 4 1.0g,H 2 O 1000mL,葡萄糖1.0g,pH7.0;

[0074] 基础培养基:NH 4 NO 3 1.00g,MgSO 4· 7H 2 O 0.5g,(NH 4 ) 2 SO 4 0.5g,KH 2 PO 4 0.5g,NaCl 0.5g,K 2 HPO 4 1.5g,H 2 O 1000mL,pH7.0;

[0075] 基础培养基加入1.5%琼脂和100mg·L -1 的联苯菊酯、甲氰菊酯和氯氰菊酯即配成相应的含农药固体培养基;

[0076] 50mg·L -1 的联苯菊酯、甲氰菊酯和氯氰菊酯乳液。

[0077] 1.2 菌株对联苯菊酯、甲氰菊酯、氯氰菊酯降解的最佳条件

[0078] 在装有100mL基础培养基的三角瓶中,混合加入联苯菊酯、甲氰菊酯、氯氰菊酯各100mg·L -1 作为碳源,接种量OD 4 15nm=0.2、30℃、pH7.0、振荡速率180r·min -1 、装液量100mL(250mL三角瓶)。最佳条件降解试验通过分别改变基本条件中的接种量(OD 415nm 为0.05,0.1,0.15,0.2,0.4)、农药浓度(50,100,150,200,250mg·L -1 )、温度(15,25,30,35,45℃)、pH值(3.0,4.0,5.0,6.0,7.0,8.0,9.0,0.0)、振荡速率(0,60,120,180,220r·min -1 )或装液量(25,50,100,150,200mL),保持其他条件不变,培养3天,进行单因素条件试验,各单因素设计以不接种为对照,每个处理设3个重复,按照实施例1方法测定菌株同时对3种菊酯农药的降解率。

[0079] 1.2.1 接种量的影响

[0080] The concentrations of bifenthrin, fenpropathrin, and cypermethrin were all 100 mg·L -1 的混合培养基,分别接种初始菌液OD 415nm 值为0....

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Abstract

The invention provides a microorganism culture that is pseudomonas diminuta and can simultaneously degrade a plurality of pyrethroid insecticides, application of the pseudomonas diminuta in pyrethroid insecticide residue degradation, and a pyrethroid insecticide residue degrading agent that is prepared by the pseudomonas diminuta. The invention obtains the high-efficiency degrading strain which can simultaneously degrade three pyrethroid insecticide residues that include bifenthrin, fenpropathrin and cypermethrin through filtering, and implements analysis, identification and degrading characteristics research of the strain so as to discover the basic insecticide degrading rules of the strain and provide scientific foundation for controlling pyrethroid insecticide residues.

Description

(1) Technical field [0001] The invention relates to the application of pseudomonas diminuta in degrading pyrethroid pesticide residues, and the degradation preparation of pyrethroid pesticide residues prepared by using the strain. (2) Background technology [0002] Pyrethroid pesticides are synthesized by simulating natural pyrethrins and contain multiple benzene ring structures. They have the characteristics of broad-spectrum, high efficiency, low toxicity, resistance to light and heat, and their number of varieties and usage are second only to Organophosphorus pesticides, which occupy the second place in the insecticide market, are widely used in the pest control of economic crops such as tea and vegetables. Bifenthrin, fenpropathrin, and cypermethrin are commonly used. However, this kind of pesticides generally have environmental stability, slow degradation rate, low degradation rate, and high residues, which have brought about food safety problems. Especially after my co...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/38A62D101/04
Inventor 谢晓梅廖敏
Owner ZHEJIANG UNIV
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