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Degradation bacterium for pyrethroid insecticides and application thereof

A technology of pyrethroids and pesticides, which is applied in the field of degrading bacteria of pyrethroids, can solve problems such as microorganisms that have not been studied for degrading dextrofenothrin, and achieve a rich germplasm resource pool. , the effect of significant application value

Active Publication Date: 2020-03-27
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, so far, no reports have been published on the study of d-cyphenothrin-degrading microorganisms

Method used

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  • Degradation bacterium for pyrethroid insecticides and application thereof
  • Degradation bacterium for pyrethroid insecticides and application thereof
  • Degradation bacterium for pyrethroid insecticides and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The separation and identification of embodiment 1 degrading bacteria

[0044] 1. Screening and isolation of d-cyphenothrin-degrading strains:

[0045] Collect activated sludge from a farmland located in Harbin City, Heilongjiang Province. After fully mixing, weigh 5g of activated sludge sample and add it to a 250mL Erlenmeyer flask containing 50mL liquid MSM medium, add d-cyphenothrin The mother liquor makes its final concentration 50mg / L. After culturing at 30°C and 200rpm for 7 days, increase the pesticide mass concentration from 50mg / L to 100mg / L according to 10% inoculum each time, and increase to 200mg / L, 400mg / L and 800mg / L in the same way . Then 800 mg / L culture solution was serially diluted and spread on the MSM solid plate containing 50 mg / L d-cyphenothrin, and cultured upside down at 30° C. for 3 days. After a single colony grows on the plate, pick a single colony and streak it on the LB plate for purification three times, and obtain a high-efficiency degra...

Embodiment 2

[0057] Example 2 Degradation Effect Experiment of Bacterial Strain HLJ-10 on D-cyphenothrin

[0058] 1. Experimental method

[0059] (1) Preparation of seed liquid: insert the purified strain HLJ-10 into LB liquid medium containing 5 mL and activate it overnight to the logarithmic phase. The second time, the obtained bacteria were used as the inoculum.

[0060] (2)Determination of degradation performance: according to the OD of the bacterial solution 600 The bacterium with a value of 0.8 was inoculated into 50 mL of MSM culture solution containing dex-cyphenothrin (50 mg / L), and no inoculation was used as the control, and each group was repeated three times. Incubate at 30°C and 200rpm constant temperature shaker for 7 days, take samples every 1d, measure the growth of strain HLJ-10, and use high performance liquid chromatography (HPLC) to measure its degradation of d-cyphenothrin.

[0061] (3) Chromatographic conditions:

[0062] HPLC: Type 2690 (Waters, USA)

[0063] Co...

Embodiment 3

[0078] Example 3 Optimum Degradation Conditions of D-cyphenothrin by Bacterial Strain HLJ-10

[0079] 1. Experimental method

[0080] The HLJ-10 strain was subjected to a single-factor degradation test, and the degradation rate of the HLJ-10 strain was determined by sequentially changing the factors affecting growth and degradation, such as temperature, pH value, inoculation amount, pesticide concentration, and oscillation rate, to determine the impact of the strain HLJ. -10 key factors for degradation rate.

[0081] Utilize Design Expert 12.0 software to carry out experimental design (table 3) according to the principle of response surface method Box-Behnken design, to influence the key factor temperature (X 1 ), pH value (X 2 ) and inoculation volume (X 3 ) is the independent variable, and the residual amount of d-cyphenothrin is the response value (Y 1 ), to establish a multiple quadratic regression equation.

[0082] According to the multiple quadratic regression equa...

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Abstract

The invention discloses a degradation bacterium for pyrethroid insecticides and application thereof. The invention finds out that Staphylococcus succinus has a degradation effect on various pyrethroidinsecticides such as d-cyphenothrin, permethrin, allethrin, bifenthrin, tetramethrin and chlorempenthrin for the first time, and a Staphylococcus succinus strain HLJ-10 which can effectively degradethe pyrethroid insecticides including d-cyphenothrin and the like is obtained by screening. On the basis of this invention, Staphylococcus succinus has a remarkable biodegradation effect on the pyrethroid insecticides including d-cyphenothrin and the like, can effectively degrade pyrethroids in a relatively wide pH value, temperature and concentration range, and can be used for repairing pollutedenvironments including water bodies, soil and the like polluted by the pyrethroid insecticides including d-cyphenothrin and the like. According to the degradation bacterium disclosed by the invention,a new development way is provided for breaking through an existing bottleneck in an existing process for managing pesticide residue caused pollution, a germplasm resources pool of the pesticide degradation bacteria is enriched and the application prospect is wide.

Description

technical field [0001] The invention belongs to the technical field of microbial degradation. More specifically, it relates to a pyrethroid insecticide-degrading bacterium and its application. Background technique [0002] Pyrethroids are a class of synthetic insecticides with high efficiency, broad spectrum, low toxicity and biodegradability, including d-cyphenothrin, permethrin, allethrin and bifenthrin. esters, tetramethrin, and permethrin, etc. In the late 1960s, especially in the 1970s, people vigorously developed pyrethroid insecticides, gradually replacing organophosphorus and carbamate pesticides that are potentially toxic to humans and mammals. For example, d-cyphenothrin, due to its low toxicity and high insecticidal activity, is frequently used in the control of indoor sanitary pests (such as: cockroaches, mosquitoes, houseflies, fleas and mites, etc.). [0003] However, the large-scale application of pesticides in agricultural production produces a large amoun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/44C02F101/38C02F101/36
CPCB09C1/10C02F3/34C02F2101/306C02F2101/36C02F2101/38C12N1/205C12R2001/44
Inventor 陈少华黄耀华林子秋张文平庞诗梅郭雨欣
Owner SOUTH CHINA AGRI UNIV
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