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A kind of degrading bacterial strain of pyrethroid insecticide and its application

A technology of pyrethroids and insecticides, which can be used in the application of environmental microorganisms and agricultural fields, and can solve the problems of polluted soil, damage to the ecological environment, damage and the like

Active Publication Date: 2021-04-13
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of pesticides can also cause damage to other non-target organisms such as silkworms, birds, bees and fish, and the use of pesticides can also damage the ecological environment by polluting soil, water, turf and other plants

Method used

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  • A kind of degrading bacterial strain of pyrethroid insecticide and its application
  • A kind of degrading bacterial strain of pyrethroid insecticide and its application
  • A kind of degrading bacterial strain of pyrethroid insecticide and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Isolation, screening and identification of degrading bacterial strain ZH-01

[0032] 1. Enrichment culture, isolation and screening of pyrethroid insecticide-degrading bacteria:

[0033] From collecting the activated sludge of sewage treatment pond in Singapore, take by weighing 5g sample and join in the above-mentioned MSM liquid culture medium that contains bifenthrin (50mg / L) in 50mL. After culturing at 30°C and 200rpm for 7 days, the mass concentration of pesticides was sequentially increased from 50mg / L to 100mg / L, 200mg / L, 400mg / L, and 800mg / L at an inoculum size of 10% each time for continuous enrichment culture. Then, the culture solution transferred for 4 times was serially diluted and spread on the LB solid plate containing 50 mg / L bifenthrin, and cultured upside down at 30°C for 2 days. After a single colony grows on the plate, pick a single colony and streak it several times to purify it, and obtain a strain of bacteria, numbered ZH-01.

[0034] ...

Embodiment 2

[0039] Example 2 Microacidophilus Stenotrophomonas ZH-01 Degradation Ability Determination of Pyrethroid Insecticides

[0040]1. Add pyrethroid insecticides such as bifenthrin, deltamethrin, beta-cyfluthrin, fenpropathrin, beta-cyhalothrin and beta-cypermethrin respectively in basal salt medium (MSM), Make the final concentration 50mg / L; the inoculum concentration is 1.0×10 7 CFU / mL of ZH-01 bacterial agent, and set the medium without inoculation as the control, culture at 30°C for 120 hours, take samples regularly, use HPLC method to determine the residual amount of pesticides, and calculate the degradation rate.

[0041] The calculation method of degradation rate is as follows:

[0042]

[0043] 2. The results are shown in Table 1.

[0044] The experimental results show that the strain ZH-01 rapidly degrades various pyrethroid insecticides in a short period of time, and the degradation rate of 50mg / L bifenthrin reaches more than 85% within 72 hours, and the degradation ...

Embodiment 3

[0047] Example 3 Microacidophilus Stenotrophomonas ZH-01 Soil Remediation Experiment

[0048] 1. Soil sample for test

[0049] The topsoil (0-20cm) of the farmland was taken from the experimental field of the teaching farm of South China Agricultural University.

[0050] After the soil samples were retrieved, they were first placed in a cool and ventilated place to dry naturally, then ground after air drying, passed through a 2mm sieve, and a certain amount of bifenthrin was dissolved in acetone, and then soaked in diatomaceous earth to completely dissolve the bifenthrin. adsorption.

[0051] The soaked diatomaceous earth is placed in a fume hood to dry, and it is mixed into the soil so that the concentration of bifenthrin in the soil is about 50 mg / kg. Take 500g of each soil sample and cultivate it in a constant temperature and humidity incubator at 30°C. 7 The inoculation amount of CFU / g was inserted into the ZH-01 degrading bacterial agent, and the addition of distilled ...

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Abstract

The invention discloses a pyrethroid insecticide degrading strain and application thereof. The degrading strain is Stenotrophomonas acidaminiphila strain ZH‑01, which was deposited in the China Center for Type Culture Collection on November 28, 2016, with a preservation number of CCTCC NO: M 2016688. The strain can effectively degrade pyrethroid insecticides such as bifenthrin in a short period of time, and the biodegradation effect is remarkable, and can be used to repair water, soil and other natural environments polluted by pyrethroid insecticides such as bifenthrin , direct application can reduce the residues of pyrethroid insecticides such as bifenthrin in water and soil by more than 80%, and solve the problem of excessive pyrethroid pesticide residues and environmental pollution in agricultural production. application prospects.

Description

technical field [0001] The invention relates to the fields of applied environmental microorganisms and agriculture. More specifically, it relates to a strain ZH-01 of Stenotrophomonas acidaminiphila which degrades pyrethroid insecticides and its production and application. Background technique [0002] The use of pesticides has played an important role in protecting crops and improving productivity. However, improper application of pesticides, especially over-dose and over-range use and irregular use of intervals, will lead to a large amount of pesticide residues in agricultural products and the environment, causing a series of food problems. safety issues and environmental safety issues. Pesticide residues are currently detected in both agricultural and non-agricultural areas, rural and urban environments, posing potentially enormous harm to human health and ecosystems. A large number of studies have shown that the impact of pesticide residues on human health includes acu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/34
CPCB09C1/10C02F3/34C02F2101/306C02F2101/34C12N1/205C12R2001/01
Inventor 陈少华李绮婷张文平林子秋黄耀华李紫怡
Owner SOUTH CHINA AGRI UNIV
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