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A kind of pyrethroid pesticide degrading strain and its application

A technology of pyrethroids and pesticides, which is applied to non-decarboxylated Lucka strain Y4 and the bacteria agent and application field produced by it, can solve the problems of unsatisfactory effect and strain degradation, etc.

Active Publication Date: 2021-01-26
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there will also be problems of strain degradation and unsatisfactory effects
In particular, d-phenothrin is a pyrethroid pesticide that has been marketed recently, and there are few research reports on the microbial degradation of d-phenothrin at home and abroad.

Method used

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  • A kind of pyrethroid pesticide degrading strain and its application
  • A kind of pyrethroid pesticide degrading strain and its application
  • A kind of pyrethroid pesticide degrading strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Isolation, screening and identification of pesticide degradation strain Y4

[0036] 1. Enrichment culture and isolation and screening of d-phenothrin-degrading bacteria:

[0037] From the farmland soil collected in the suburbs of Harbin, Heilongjiang, 5 g of soil samples were weighed and added to 50 mL of the above-mentioned MSM liquid medium containing d-phenothrin (50 mg / L). After culturing at 30°C and 200rpm for 7 days, the mass concentration of pesticides was sequentially increased from 50mg / L to 100mg / L, 200mg / L, 400mg / L, and 800mg / L at an inoculum size of 10% each time for continuous enrichment culture. Then, the culture medium transferred for 4 times was serially diluted and spread on the LB solid plate containing 50 mg / L d-phenothrin, and cultured upside down at 30°C for 2 days. After a single colony grows on the plate, pick a single colony and streak it several times for purification, and obtain a strain of bacteria, numbered Y4.

[0038] 2. Morphol...

Embodiment 2

[0043] Example 2 Decarboxylation Luckae Y4 Degradation Ability Determination of D-Phenothrin

[0044] 1. Experimental method

[0045] (1) Seed liquid preparation:

[0046] The purified strain Y4 was inserted into 5 mL of LB liquid medium for overnight activation to the logarithmic phase. After centrifugation at 4° C., the bacteria were washed with physiological saline (0.9% NaCl), and the resulting bacteria were used as inoculum.

[0047] (2) Degradation performance determination:

[0048] Press 1.0×10 7 The inoculum amount of CFU / mL wet thalline was inoculated into 50 mL of MM medium containing d-phenothrin (50 mg / L) respectively, and no inoculation was used as a control respectively, and each group was repeated three times. Incubate at 30°C, 200rpm constant temperature shaker for 7 days, take samples once on the 1st, 3rd, 5th, and 7th day respectively, and measure the OD with a spectrophotometer 600 The value represents the growth of strain Y4, and its degradation of d-phe...

Embodiment 3

[0074] Example 3 Decarboxylusella Y4 Degradation Effect of D-Phenothrin in Soil

[0075] 1. Soil sample for test

[0076] The topsoil (3-10 cm) of the farmland was taken from the experimental field of the teaching farm of South China Agricultural University.

[0077] After the soil samples were taken back, they were first placed in a cool and ventilated place to dry naturally, then ground after air drying, passed through a 2mm sieve, and a certain amount of d-phenothrin was dissolved in acetone, and then soaked in diatomaceous earth to make d-phenyl ether Permethrin is completely adsorbed. The soaked diatomaceous earth was placed in a fume hood to dry, and it was mixed into the soil so that the final concentration of d-phenothrin in the soil was 50 mg / kg. Take 500g of soil samples and culture them in a constant temperature and humidity incubator at 30°C, press 1.0×10 7 The inoculation amount of CFU / mL was inserted into the Y4 degrading bacteria suspension, and distilled wat...

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Abstract

The invention discloses a degrading bacterial strain of pyrethroid pesticides such as d-phenothrin and the application thereof. The degrading strain is a non-decarboxylated Leclercia adecarboxylata strain Y4, which was deposited in the Guangdong Microbial Culture Collection Center on September 4, 2019, with a preservation number of GDMCC No: 60762. The present invention discloses for the first time the degradation effect of non-decarboxyluckeria on d-phenothrin pesticide, and obtains the above-mentioned high-efficiency and fast-degrading strain Y4 through screening, and the bacteria also has fast growth speed, simple cultivation method, strong adaptability, It is not easy to mutate, and can be used as an excellent pesticide-degrading bacteria in the bioremediation of polluted environments such as water bodies and soils. The invention provides a new development approach for breaking the existing bottleneck of controlling pesticide residue pollution, enriches the germplasm resource bank of pesticide-degrading bacteria, and has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of pesticide pollution control. More specifically, it relates to a non-decarboxylated Leclercia adecarboxylata strain Y4 that degrades d-phenothrin pesticide, and its production and application. Background technique [0002] Pyrethroid pesticides are a class of artificially synthesized chemicals, including d-phenothrin, which has not been marketed for a long time. Because of its low toxicity to humans and animals, it is relatively safe to humans and other non-target organisms, so it is becoming more and more popular worldwide. It is more and more widely used in fields such as agricultural production, household sanitation and urban anti-mosquito. [0003] In recent years, with the increase in the use of such pesticides, the food and environmental pollution problems caused by their residues have become more and more serious. Microbial degradation is an effective measure to remove pesticide residues. It has t...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/30
CPCB09C1/10C02F3/34C02F2101/306C12N1/20C12N1/205C12R2001/01
Inventor 陈少华李绮婷崔晶涵冯彦媚占卉黄耀华
Owner SOUTH CHINA AGRI UNIV
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