A kind of degrading bacteria of pyrethroid insecticide and its application

A technology for pyrethroids and insecticides, which is applied in the field of pyrethroid insecticides degrading bacteria, can solve the problems that have not yet been studied on d-cyphenothrin degrading microorganisms, and enrich the germplasm resource bank Effect

Active Publication Date: 2021-06-15
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, so far, no reports have been published on the study of d-cyphenothrin-degrading microorganisms

Method used

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  • A kind of degrading bacteria of pyrethroid insecticide and its application
  • A kind of degrading bacteria of pyrethroid insecticide and its application
  • A kind of degrading bacteria of pyrethroid insecticide and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The separation and identification of embodiment 1 degrading bacteria

[0044] 1. Screening and isolation of d-cyphenothrin-degrading strains:

[0045] Collect activated sludge from a farmland located in Harbin City, Heilongjiang Province. After fully mixing, weigh 5g of activated sludge sample and add it to a 250mL Erlenmeyer flask containing 50mL liquid MSM medium, add d-cyphenothrin The mother liquor makes its final concentration 50mg / L. After culturing at 30°C and 200rpm for 7 days, increase the pesticide mass concentration from 50mg / L to 100mg / L according to 10% inoculum each time, and increase to 200mg / L, 400mg / L and 800mg / L in the same way . Then 800 mg / L culture solution was serially diluted and spread on the MSM solid plate containing 50 mg / L d-cyphenothrin, and cultured upside down at 30° C. for 3 days. After a single colony grows on the plate, pick a single colony and streak it on the LB plate for purification three times, and obtain a high-efficiency degra...

Embodiment 2

[0057] Example 2 Degradation Effect Experiment of Bacterial Strain HLJ-10 on D-cyphenothrin

[0058] 1. Experimental method

[0059] (1) Preparation of seed liquid: insert the purified strain HLJ-10 into LB liquid medium containing 5 mL and activate it overnight to the logarithmic phase. The second time, the obtained bacteria were used as the inoculum.

[0060] (2)Determination of degradation performance: according to the OD of the bacterial solution 600 The bacterium with a value of 0.8 was inoculated into 50 mL of MSM culture solution containing dex-cyphenothrin (50 mg / L), and no inoculation was used as the control, and each group was repeated three times. Incubate at 30°C and 200rpm constant temperature shaker for 7 days, take samples every 1d, measure the growth of strain HLJ-10, and use high performance liquid chromatography (HPLC) to measure its degradation of d-cyphenothrin.

[0061] (3) Chromatographic conditions:

[0062] HPLC: Type 2690 (Waters, USA)

[0063] Co...

Embodiment 3

[0078] Example 3 Optimum Degradation Conditions of D-cyphenothrin by Bacterial Strain HLJ-10

[0079] 1. Experimental method

[0080] The HLJ-10 strain was subjected to a single-factor degradation test, and the degradation rate of the HLJ-10 strain was determined by sequentially changing the factors affecting growth and degradation, such as temperature, pH value, inoculation amount, pesticide concentration, and shaking rate, to determine the impact of the strain HLJ. -10 key factors for degradation rate.

[0081] Utilize Design Expert 12.0 software to carry out experimental design (table 3) according to the principle of response surface method Box-Behnken design, to influence the key factor temperature (X 1 ), pH value (X 2 ) and inoculation volume (X 3 ) is the independent variable, and the residual amount of d-cyphenothrin is the response value (Y 1 ), to establish a multiple quadratic regression equation.

[0082] According to the multiple quadratic regression equation...

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Abstract

The invention discloses a pyrethroid insecticide degrading bacterium and application thereof. The present invention finds for the first time that Staphylococcus succinus kills various pyrethroids such as d-cyphenothrin, permethrin, allethrin, bifenthrin, tetramethrin and permethrin. The insecticide has degradation effect, and a Staphylococcus succinerus strain HLJ‑10 that efficiently degrades pyrethroid insecticides such as dex-cyphenothrin was screened. Based on the present invention, the biodegradation effect of Staphylococcus succinerus on pyrethroid insecticides such as d-cyphenothrin is remarkable, and pyrethroids are efficiently degraded in a wide range of pH, temperature and concentration, and can be used for repairing Water bodies and soils polluted by pyrethroid insecticides such as d-cyphenothrin pollute the environment. The invention provides a new development approach for breaking the existing bottleneck of controlling pesticide residue pollution, enriches the germplasm resource bank of pesticide-degrading bacteria, and has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of microbial degradation. More specifically, it relates to a pyrethroid insecticide-degrading bacterium and its application. Background technique [0002] Pyrethroids are a class of synthetic insecticides with high efficiency, broad spectrum, low toxicity and biodegradability, including d-cyphenothrin, permethrin, allethrin and bifenthrin. esters, tetramethrin, and permethrin, etc. In the late 1960s, especially in the 1970s, people vigorously developed pyrethroid insecticides, gradually replacing organophosphorus and carbamate pesticides that are potentially toxic to humans and mammals. For example, d-cyphenothrin, due to its low toxicity and high insecticidal activity, is frequently used in the control of indoor sanitary pests (such as: cockroaches, mosquitoes, houseflies, fleas and mites, etc.). [0003] However, the large-scale application of pesticides in agricultural production produces a large amoun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/44C02F101/38C02F101/36
CPCB09C1/10C02F3/34C02F2101/306C02F2101/36C02F2101/38C12N1/205C12R2001/44
Inventor 陈少华黄耀华林子秋张文平庞诗梅郭雨欣
Owner SOUTH CHINA AGRI UNIV
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