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Uses of enterobacter aerogenes in degrading pyrethroid pesticide residue and preparation thereof

A technology of pyrethroids and Enterobacter aerogenes, which is applied in the field of application and preparation of Enterobacter aerogenes in degrading pyrethroid pesticide residues, and can solve the problem of few microorganisms

Inactive Publication Date: 2009-04-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most microorganisms degrade one or a few pesticides, and there are few reports on microorganisms that can degrade multiple pyrethroid pesticides at the same time.

Method used

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  • Uses of enterobacter aerogenes in degrading pyrethroid pesticide residue and preparation thereof
  • Uses of enterobacter aerogenes in degrading pyrethroid pesticide residue and preparation thereof
  • Uses of enterobacter aerogenes in degrading pyrethroid pesticide residue and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Screening and identification of degrading strains

[0040] 1 Materials and methods

[0041] 1.1 Medium and Reagents

[0042] Enrichment medium: peptone 10g, NaCl 1.0g, KH 2 PO 4 1.0g, H 2 O 1000mL, glucose 1.0g, pH 7.0;

[0043] Basal medium: NH 4 NO 3 1.00g, MgSO 4 .7H 2 O 0.5g, (NH 4 ) 2 SO 4 0.5g, KH 2 PO 4 0.5g, NaCl0.5g, K 2 HPO 4 1.5g, H 2 O1000mL, pH7.0;

[0044] Add 1.5% (w / v) agar and 100 mg.L to the basal medium -1 The bifenthrin, cypermethrin and cypermethrin are prepared into corresponding pesticide-containing solid medium;

[0045] 50mg·L -1 of bifenthrin, cypermethrin and cypermethrin emulsions.

[0046] 1.2 Determination of Degradation Efficacy of Bifenthrin, Cypermethrin and Cypermethrin by Strain

[0047] The purified single strain was calculated as the bacterial mass OD 415nm = 0.2 inoculated into 100mL containing 100mg·L of bifenthrin, cypermethrin and cypermethrin -1 In a 250 mL conical flask of sterile liquid enr...

Embodiment 2

[0072] Example 2: Optimization of degradation conditions

[0073] 1 Materials and methods

[0074] 1.1 Medium and Reagents

[0075] Enrichment medium: peptone 10g, NaCl 1.0g, KH 2 PO 4 1.0g, H 2 O 1000mL, glucose 1.0g, pH 7.0;

[0076] Basal medium: NH 4 NO 3 1.00g, MgSO 4 .7H 2 O 0.5g, (NH 4 ) 2 SO 4 0.5g, KH 2 PO 4 0.5g, NaCl0.5g, K 2 HPO 4 1.5g, H 2 O1000mL, pH7.0;

[0077] The basal medium was added with 1.5% (w / v) agar and 100 mg·L -1 The bifenthrin, cypermethrin and cypermethrin are prepared into corresponding pesticide-containing solid medium;

[0078] 50mg·L -1 of bifenthrin, cypermethrin and cypermethrin emulsions.

[0079] 1.2 Determination of Degradation Efficacy of Bifenthrin, Cypermethrin and Cypermethrin by Strain

[0080] The purified Enterobacter aerogenes M6R9 strain was measured by OD 415nm = 0.2 inoculated into 100mL containing 100mg·L of bifenthrin, cypermethrin and cypermethrin -1 In a 250 mL conical flask of sterile liquid enrich...

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Abstract

The invention provides application of a microbe strain-Enterobacter aerogenes capable of degrading various pyrethroid pesticides simultaneously to the degrading of pyrethroid pesticide residue, and a preparation for degrading the pyrethroid pesticide residue prepared by the strain. An efficient degrading strain is obtained through screening to simultaneously degrade three pyrethroid pesticide residues, namely bifenthrin, fenpropathrin and cypermethrin, and is analyzed and researched in degrading characteristic, so as to disclose the basic rule of the strain for degrading the pesticide and provide scientific basis for controlling pyrethroid pesticide residue.

Description

(1) Technical field [0001] The invention relates to the application of Enterobacter aerogenes in degrading pyrethroid pesticide residues, and a pyrethroid pesticide residue degrading preparation prepared by using the strain. (2) Background technology [0002] Pyrethroid pesticides are a class of pesticides synthesized by simulating natural pyrethroids and containing multiple benzene ring structures. They have the characteristics of broad-spectrum, high efficiency, low toxicity, light resistance and heat resistance. Organophosphorus pesticides, which occupy the second place in the pesticide market, are widely used in the control of economic crop pests such as tea and vegetables. However, these pesticides generally have environmental stability, slow degradation rate, low degradation rate, and high residue, which brings food safety problems. Especially after my country's entry into the WTO, the "tariff barriers" were dismantled, and the maximum residues (MRL) standards for pes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A62D3/02A62D101/04
Inventor 谢晓梅廖敏
Owner ZHEJIANG UNIV
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