Klebsiella inoculum capable of biodegrading residual chlorpyrifos pesticide on surfaces of garden stuffs and soil

A Klebsiella, biodegradable technology for use in microorganism-based methods, restoration of contaminated soils, microbes, etc.

Inactive Publication Date: 2014-01-01
SICHUAN UNIV
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Problems solved by technology

It has been reported that hypochlorite treatment of pesticide residues may lead to the formation of some toxic or carcinogenic organic chlorine compounds that stick to the surface of fruits and vegetables, so the use is subject to certain restrictions

Method used

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  • Klebsiella inoculum capable of biodegrading residual chlorpyrifos pesticide on surfaces of garden stuffs and soil

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Embodiment 1

[0015] Collect the fruit and vegetable production base in Longquanyi District, Chengdu, spray the soil with chlorpyrifos pesticide for many years, adopt enrichment culture, select medium culture, and finally isolate and purify a strain of bacteria, and then use high-concentration 100-1000mg / L chlorpyrifos medium as the only carbon Source domesticated bacteria. Through 16SrDNA sequence analysis, it was found that strain B and Klebsiella The sequence similarity of 2009I6 is 99%, and Klebsiella sp. . The sequence similarity of MS6 is also 99%, GenBank accession number HQ836365. The 10 strains obtained from the primary screening were activated, inserted into 100mg / l chlorpyrifos basic salt medium, cultured at 37°C and 180r / min for 10 days, and the degradation rate was measured. Through re-screening, the strain DSP-5 with the highest degradation rate was obtained. The main biological characteristic is G - , Facultative anaerobic bacteria, capsulated, non-spore-forming. The ...

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Abstract

A microbial strain capable of degrading chlorpyrifos pesticide residue in soil with the chlorpyrifos pesticide residue is isolated. The microbial strain is klebsiella sp. dsp-B by identification, Genbank accession number is HQ836365, and the strain preservation number is CCTCC: NO: M2012477. The characteristic of chlorpyrifos pesticide degradation of klebsiella is utilized for development of biodegrading inoculum applicable to the surfaces of garden stuffs and soil. The klebsiella inoculum belongs to the field of microbial environment modification, and the process flow comprises: purified strain preserving, shaking culture, seeding tank enlargement culture, fermentation tank secondary enlargement, and product split charging. The inoculum is simple in preparation technology, low in production cost and easy to popularize and apply, and the application characteristic is direct spraying. The klebsiella inoculum is capable of fast effectively degrading residual chlorpyrifos pesticide on the surfaces of garden stuffs and soil, so that the exceeding standard problem of the residue is solved; and the klebsiella inoculum has direct economical value for production nontoxic nuisance-free greed agricultural products.

Description

technical field [0001] The invention belongs to the technical field of microbial environment restoration, and in particular relates to a Klebsiella dsp-B strain and a biodegradation method for chlorpyrifos pesticide residues on soil and fruit and vegetable surfaces. [0002] Background technique [0003] Pesticides are an indispensable means of production in modern agriculture. They play a huge role in reducing crop pests and diseases, increasing the yield of agricultural products, and improving the quality of agricultural products, thereby improving agricultural production efficiency. my country is a large agricultural country with a huge amount of pesticide production and use. In terms of pesticide production, my country's pesticide production has shown a continuous and rapid growth trend in recent years. National Bureau of Statistics data shows that the national pesticide output in 2011 has reached 2.648 million tons. Regarding the use of pesticides, the National Agric...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N25/32B09C1/10C12R1/22
Inventor 徐恒李创唐敦义
Owner SICHUAN UNIV
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