Soil remediation agent and application thereof
A technology of soil remediation agent and adjuvant, which is applied in the field of soil remediation, and can solve problems such as endangering the soil environment and changing bioavailability
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[0026] In the present invention, the preparation method of described Paenibacillus Kliben comprises the following steps:
[0027] 1) activating the Paenibacillus species of Klebben, inoculating it in a primary liquid medium for primary liquid culture, and obtaining a primary seed liquid;
[0028] 2) inoculating the primary seed liquid obtained in the step 1) in a secondary liquid medium to carry out secondary liquid culture to obtain a secondary seed liquid;
[0029] 3) inoculate the secondary seed liquid obtained in the step 2) into a tertiary liquid culture medium for tertiary liquid culture, and obtain Paenibacillus Kleben after separation and drying treatment.
[0030] In the present invention, the preserved Paenibacillus species are inoculated into eggplant-shaped bottles equipped with activated medium, and the culture conditions are preferably 28-32°C for 8-12h, more preferably 29-31°C. 9-11 hours, most preferably 10 hours at 30°C.
[0031] In the present invention, th...
Embodiment 1
[0084] Preparation of the primary seed liquid of Paenibacillus Kleben: Inoculate the preserved Paenibacillus Kleben strains into eggplant-shaped bottles with activation medium, and culture them at 28°C for 12 hours to complete the activation of the strains; The activated Paenibacillus strain was inoculated in the Paenibacillus Kleben primary liquid culture medium according to the inoculation amount of 1%, and the primary liquid medium included the following components in mass content: 3% sucrose, nitric acid Sodium 0.15%, dipotassium hydrogen phosphate 0.1%, magnesium sulfate 0.05%, potassium chloride 0.05%, iron sulfate 0.002%, and the balance is water. The temperature of the primary liquid culture of Paenibacillus Kliben is 30°C, and the initial pH value is 7.5. 3 / h ventilation for aeration culture, after 24 hours of liquid culture, according to 20m 3 The aeration was carried out at an aeration rate of 1 / h, and after 30 hours of light cultivation, the cultivation was stopp...
Embodiment 2
[0088] The preparation of soil remediation agent: in parts by weight, 10 parts of talcum powder, 55 parts of kaolin, 3.75 parts of Paenibacillus Kliben prepared in Example 1, 2.9 parts of Bacillus subtilis, 1.7 parts of Trichoderma harzianum, 0.8 part Lactobacillus acidophilus, 0.8 parts of yeast, 3.3 parts of plant ash, 3.3 parts of wood chips, 3.3 parts of vermiculite, 10 parts of glucose and 5 parts of corn starch were divided into 4 piles, and the first pile of talcum powder was mixed with the first pile of kaolin , mixed with the first part of plant ash, then mixed with the first part of wood chips, then mixed with the first part of vermiculite, then mixed with the first pile of Bacillus subtilis, then mixed with the first pile of Paenibacillus Kleben, and then Mix with first pile of Trichoderma harzianum, then with first pile of Lactobacillus acidophilus, then with first pile of yeast, then with first pile of glucose, then with first pile of cornstarch, then with second p...
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