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Pesticide degrading agent of esters specially for vegetable and fruit

A pesticide degradation and ester technology, which is applied in the protection device against harmful chemicals, etc., can solve the problems of not having a broad spectrum, low cleaning effect of pesticides, etc.

Inactive Publication Date: 2004-09-08
INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to overcome the defects of the prior art that the cleaning effect of pesticides is low and does not have broad spectrum, thereby providing a special ester pesticide degradation agent for fruits and vegetables, which includes the high-density culture of detoxification engineering bacteria. Dry cell powder of detoxifying enzymes that degrade organophosphates, organochlorates, carbamates and pyrethroids on fruits and vegetables

Method used

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  • Pesticide degrading agent of esters specially for vegetable and fruit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A kind of detoxification engineered bacterium (ZL97100018.2) was cultured in a universal stirred bioreactor with high density and the wet cells obtained by centrifuging the detoxified engineered bacterium (ZL00123772.1) were fermented, freeze-dried and pulverized. This dry cell powder 6g is mixed with citric acid 4.0g evenly, and is made into 40 mesh granules in the granulator. 16g of sodium bicarbonate, 1g of Triton-X 100, and 0.3g of sodium carboxymethylcellulose were mixed uniformly, and compressed in a tablet machine to obtain a tablet with a thickness of 1.0cm, a diameter of 1.2cm, and a weight of 2.0g. Mix the tablet with the granules containing dry cell powder, and press it in a tablet machine at a temperature of 25°C to obtain a tablet with a thickness of 1.0cm, a diameter of 1.2cm, and a weight of 2.8g, to obtain a special ester pesticide for vegetables and fruits. effervescent tablet.

[0031] Use this effervescent tablet to conduct a degradation test on resi...

Embodiment 2

[0041] A kind of detoxification engineered bacterium (ZL97100018.2) was cultured in a universal stirred bioreactor with high density and the wet cells obtained by centrifuging the detoxified engineered bacterium (ZL00123772.1) were fermented, freeze-dried and pulverized. This dry cell powder 10g is mixed with citric acid 4.0g evenly, and is made into 10 mesh granules in a granulator. Mix 40g of sodium bicarbonate, 5g of Triton-X 100, and 0.4g of sodium carboxymethylcellulose, and press into a tablet in a tablet machine to form a tablet with a thickness of 1.4cm, a diameter of 1.6cm, and a weight of 4.0g. Mix the tablet with the granules containing dry cell powder, and press it in a tablet machine at a temperature of 25°C, and press it into a tablet with a thickness of 1.4cm, a diameter of 1.6cm, and a weight of 4.0g to obtain a special ester pesticide for vegetables and fruits. effervescent tablet.

[0042] Using the method in Example 1, the effervescent tablet was used to ca...

Embodiment 3

[0049] A kind of detoxification engineered bacterium (ZL97100018.2) was cultured in a universal stirred bioreactor with high density and the wet cells obtained by centrifuging the detoxified engineered bacterium (ZL00123772.1) were fermented, freeze-dried and pulverized. This dry cell powder 12g is mixed with citric acid 10.0g evenly, and is made into 40 mesh granules in the granulator. Mix 40.0g of sodium bicarbonate, 4g of sodium dodecylbenzenesulfonate, and 0.8g of sodium carboxymethylcellulose, and press it into a tablet with a thickness of 1.0cm, a diameter of 1.2cm, and a weight of 2.0g. tablet. Mix the tablet with the granules containing dry cell powder, press it in a tablet machine at a temperature of 30°C, and press it into a tablet with a thickness of 1.0 cm, a diameter of 1.2 cm, and a weight of 2.0 g to obtain a special ester pesticide degradation agent for fruits and vegetables effervescent tablet.

[0050] Using the method in Example 1, using the effervescent t...

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Abstract

The pesticide degrading agent of esters specially for vegetable and fruit includes cell powder, anionic surfactant, citric acid, sodium bicarbonate and CMCNa. The cell powder is prepared with the microbe strain of the preservation number of CGMCC No. 0290 and through high density culture with detoxicated engineering bacteria to produce detoxification enzyme, fermentation, centrifugation, freeze drying and crushing. The pesticide degrading agent of the present invention can degrade the residual organic phosphate and pyrethrin pesticides in vegetable and fruit with relatively high effect.

Description

technical field [0001] The present invention relates to an ester pesticide degradation agent for fruits and vegetables, in particular to an ester pesticide degradation agent for fruits and vegetables including detoxification enzymes (ZL00123772.1, ZL97100018.2) produced by high-density culture of detoxification engineering bacteria. technical background [0002] Pesticides are necessary means of production in agricultural production, and at the same time they are toxic chemicals that are harmful to the environment. The widespread use of pesticides has played a very important role in eliminating pests and diseases and ensuring stable and high yields of crops. But on the other hand, after a large amount of toxic pesticides enter the environment, they will definitely pollute and destroy the ecological environment, and bring adverse effects on human life and health. At present, chemical pesticides, factory "three wastes", urban garbage, chemical fertilizers and human factors ar...

Claims

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Application Information

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IPC IPC(8): A62D3/02A62D101/04
Inventor 乔传令郑玉芝张建亮
Owner INST OF ZOOLOGY CHINESE ACAD OF SCI
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