20 results about "Detoxification enzymes" patented technology

The invention discloses a highland barley wine composition with a liver protection effect as well as a preparation method and application of the highland barley wine composition, and relates to the technical field of traditional Chinese medicine compositions. The highland barley wine comprises the following raw materials in parts by weight: 30-600 parts of highland barley wine, 2-10 parts of dandelion, 3-10 parts of dried lalang grass rhizome, 2-15 parts of orange peel, 6-15 parts of cinnamon, 5-15 parts of poria cocos, 2-10 parts of platycodon grandiflorum, 3-10 parts of liquorice, 3-10 parts of kudzu vine root and 3-15 parts of hawthorn. According to the highland barley wine composition, the problem that heat-sensitive liver protection components are easy to degrade due to high temperature in a traditional process is solved through a stepped heating sterilization mode, high-activity components are reserved, and it is ensured that effective components play a synergistic effect in key links such as hepatic cell oxidative stress inhibition and detoxification enzyme activity adjustment; a plurality of traditional Chinese medicine raw material components and the highland barley wine form an anti-oxidation-anti-inflammatory-regeneration-promoting triple synergistic protection network, so that the liver protection function of the highland barley wine is enhanced.

The present application relates to the field of bioengineering and microbial technology, and relates to a yeast surface display construction method of a double-enzyme system. AGA1 The gene promoter is replaced by a strong constitutive promoter TEF1 and a glucose-responsive promoter HXT7 ; a double-enzyme free expression vector containing a galactose-inducible promoter GAL1 driven detoxification enzyme gene is constructed, and is transformed into a modified chassis cell to obtain a double-enzyme co-display engineering bacterium; the chassis protein AGA1 is expressed in a culture medium, and YtdepA and YtdepB are induced in a galactose-induced culture medium, so that the YtdepA and YtdepB are co-displayed on the yeast surface through the coupling mechanism of AGA1-AGA2. The strong constitutive promoter TEF1 and the glucose-repressed promoter HXT7 are coupled with the galactose-inducible promoter GAL1 respectively, so that the chassis protein and the detoxification enzyme are matched in the expression timing and intensity, and the detoxification enzyme is displayed on the yeast surface.

This invention discloses a vomitoxin detoxification enzyme, its encoding gene, and its applications, belonging to the field of molecular biology. This invention discovered a wheat SOT gene, TaSOT2b. The TaSOT2b protein was obtained through in vitro induced expression, and its amino acid sequence is shown in SEQ ID NO.1. This invention found that this protein possesses in vitro enzymatic activity, capable of modifying DON into SON-3-sulfate, and can act as a vomitoxin detoxification enzyme. It has potential application value and reference significance for the scientific prevention and control of wheat scab and DON toxin contamination, and has broad application prospects in agriculture, feed, and industry.

The application discloses a bamboo shoot enzyme slurry based on bamboo shoot slurry zymolysis and a preparation method thereof, relates to the technical field of fermented food, and the preparation method comprises the following steps: 1) bamboo shoot pretreatment and residual heat zymolysis; 2) in-situ detoxification and fermentation; 3) filtration and seasoning treatment; and 4) sterile packaging and storage. The application integrates zymolysis, directional detoxification and segmented fermentation, synchronously adds detoxification enzymes and complex enzyme preparations, starts the reaction by using the residual heat of fixation, shortens the process flow by more than 40%, and significantly reduces energy consumption. Through the in-situ detoxification and fermentation mechanism, toxins such as tannin, oxalic acid and cyanogenic glycosides are synchronously degraded in the microbial metabolism process, and the safety and efficiency are simultaneously improved. The hierarchical filtration and flexible deployment technology can be used to accurately produce low-sugar and sugar-free products in the same production line, breaks through the single limitation of traditional process products, and realizes the unity of high efficiency, safety and market diversification demand.

The invention belongs to the field of chemical ecology of agricultural pests and pesticide science, and relates to an insecticide composition for improving the sensitivity of agricultural pests to insecticides and application of the insecticide composition. Folic acid is applied to a pest feeding substrate, a host plant or a feed, so that the sensitivity of pests to various insecticides (including neonicotinoids, pyrimidines, pyrethroids, organophosphorus and the like) can be remarkably enhanced. It is found that folic acid can regulate and control a carbon metabolism network in pests and inhibit cytochrome P450 and other detoxification enzyme related pathways, and then the endurance capacity of the pests to insecticides is reduced. The composition and the method are verified in seven important pests such as brown planthopper, sogatella furcifera, spodoptera frugiperda, cotton aphid, chilo suppressalis, panonychus citri and thrips, have the advantages of improving the pesticide effect, reducing the pesticide application amount and delaying the resistance evolution, and can be widely applied to comprehensive treatment of agricultural pests.

The invention discloses a W-5 strain for improving pesticide tolerance of natural enemy insects and a culture method of the W-5 strain, the W-5 strain can well grow in a lowest-salt culture medium with lambda-lambda-cyhalothrin as a unique carbon source, the OD600 of a cultured bacteria solution is continuously increased, and the degradation rate of cyhalothrin pesticide reaches 98.24%. Furthermore, after the tenebrio molitor treated by the W-5 is fed to the hunting fuscipes, the activity of two types of key detoxifying enzymes, namely glutathione S-transferase and cytochrome P450, in the body of the hunting fuscipes can be remarkably improved. After the strain is continuously fed for one week, the W-5 is successfully colonized in intestinal tracts of the hpactor fuscipes, and the survival rate of the hpactor fuscipes under the stress of lambda-lambda-cyhalothrin is increased to 90.625%. Therefore, through double mechanisms of'direct microbial degradation 'and'host detoxification metabolism activation', the W-5 synergistically enhances the resistance of the Harpactor fuscipes to the lambda-lambda-cyhalothrin.

This invention concerns a mycotoxin detoxification-based feed additive for poultry, particularly broiler chickens, aimed at addressing the decline in feed nutrient utilization and performance due to aflatoxin B1 and ochratoxin A contamination. This invention relates to the use of a multicomponent feed additive formulation that combines inorganic mycotoxin binders, mycotoxin modifying components in the form of biological agents and / or detoxification enzymes, and supporting components in the form of natural antioxidants that work synergistically through adsorption and biotransformation mechanisms in the digestive tract. This formulation functions to reduce the toxicity of aflatoxin B1 and ochratoxin A thereby optimizing the utilization of feed nutrients. The application of this feed additive at an effective dose allows for increased feed utilization efficiency and performance of broiler chickens consuming mycotoxin-contaminated feed, as well as supporting livestock health and productivity in a practical and efficient manner.This invention can be applied in poultry feed management, especially in conditions with a high risk of mycotoxin contamination.

The invention discloses a sorbose dehydrogenase mutant capable of efficiently degrading vomitoxin, and belongs to the technical field of agricultural biology. The mutant is based on wild type SDH, through rational design and a directed evolution strategy, the mutant comprises three mutation sites F103A, T492E and N427A, the amino acid sequence and the nucleotide sequence of the mutant are SEQ ID NO.1 and SEQ ID NO.2 respectively, the mutant SDHF103A / T492E / N427A can efficiently degrade DON without depending on phenazine methyl sulfate (PMS), and the degradation activity of the mutant SDHF103A / T492E is improved by about two times compared with that of a double mutant SDHF103A / T492E under the condition that PMS does not exist. The method can be applied to preparation of the vomitoxin detoxification enzyme, and has a good application prospect in the aspect of biological detoxification of DON in feeds, grains and food.

The invention relates to aldehyde ketoreductase and application thereof in degradation and detoxification of 3-keto-DON, and belongs to the technical field of bioengineering. The amino acid sequence of the aldoketoreductase is as shown in SEQ ID NO. 1. The aldoketoreductase can realize heterologous high-efficiency expression in a prokaryotic expression system, and is easy for later-stage purification of detoxification enzyme and preparation of enzyme preparations. The aldehyde ketoreductase disclosed by the invention can be used for effectively degrading the 3-keto-DON toxin, and the degradation rate reaches 86.5% or above. Compared with physical detoxification, biological detoxification is more environmentally friendly, secondary pollution is avoided, and the efficient 3-keto-DON detoxification capacity has great application potential in the field.

The invention discloses a thermostable ochratoxin A (OTA) degrading enzyme as well as application, a method and a product thereof, and belongs to the technical field of microorganisms. After OTA degrading enzyme from a thermophilic microorganism Rhodotherm marinus is optimized, enzyme capable of effectively catalyzing ochratoxin A to be degraded into non-toxic substances is obtained through escherichia coli heterologous expression and purification, the specific activity of the enzyme reaches 6666 U / mg, the half-life period at 90 DEG C reaches 7.91 min, and the enzyme is superior to all known OTA detoxification enzymes. The OTA degrading enzyme successfully solves the technical problems that the OTA degrading enzyme in the prior art is relatively poor in stability and cannot meet relatively harsh industrial environmental conditions.

The present application relates to the technical field of biological enzymes, and particularly relates to a detoxification enzyme Novel porin as well as a preparation method and application thereof.The detoxification enzyme Novel porin is a microorganism-derived enzyme, and an amino acid sequence of the detoxification enzyme is shown as SEQ ID NO.1.The detoxification enzyme exhibits extremely high degradation activity on mycotoxins.Especially in terms of degradation efficiency on AFB1 and ZEN, the degradation efficiency is significantly better than that of microorganisms or other detoxification enzymes.The detoxification enzyme is used for degradation of mycotoxins, and has the advantages of high efficiency, short cycle, high safety, strong specificity and environmental friendliness, etc.Compared with the microbial degradation method, the mycotoxins are degraded by the detoxification enzyme, so that the harm caused by the toxicity of the microorganisms themselves can be avoided, and the change of the raw material properties and the sense organ is avoided, and the degradation efficiency is greatly improved.

The application discloses a heat-stable ochratoxin A (OTA) degradation enzyme and application, method and product, and belongs to the technical field of microorganisms. Rhodothermus marinus After OTA degradation enzyme from thermophilic microorganisms is optimized, the OTA degradation enzyme is expressed in E. coli in a heterologous manner, and an enzyme capable of effectively catalyzing ochratoxin A to degrade into a non-toxic substance is obtained through purification, the specific activity of the enzyme reaches 6666 U / mg, the half-life of the enzyme at 90 DEG C is up to 7.91 min, and the enzyme is superior to all known OTA detoxification enzymes. The technical problem that OTA degradation enzymes in the prior art have poor stability and cannot meet relatively harsh environmental conditions in the industry is successfully solved.

The invention discloses a drug-resistant carbendazim composite suspension and application thereof, and belongs to the technical field of agriculture and biochemistry. The suspension comprises 5.0%-8.0% of carbendazim, 3.0%-5.0% of a drug-resistant synergist, 2.0%-4.0% of a dispersant, 1.5%-3.0% of a stabilizer, 4.0%-6.0% of a carrier material and the like. Wherein the anti-drug-resistance synergist is prepared by compounding glycyrrhetinic acid stearate and alpha-linolenic acid glyceride, and the drug resistance of carbendazim is synergistically overcome by inhibiting the activity of pathogenic bacteria detoxification enzyme and destroying the structure of a cell membrane respectively; the stabilizer is a compound system of trehalose ester and polyvinyl butyral, so that the stability of the suspension rate is ensured; the carrier material is mesoporous silica loaded La < 3 + >, and has a slow release function and a synergistic bactericidal effect. Experiments show that the preparation has a good inhibition effect on drug-resistant strains, is excellent in physical and chemical stability, and can be widely applied to the fields of prevention and treatment of rice sheath blight disease, citrus penicilliosis and the like.

The invention relates to the technical field of gene editing, in particular to a gRNA composition of a targeted CYP392E1 gene for preventing and treating tetranychus urticae and application of the gRNA composition. The CYP392E1 gene of which the nucleotide sequence is shown as SEQ ID NO.1 and any one of the following applications of gRNA of which the nucleotide sequence is shown as SEQ ID NO.2: 1) preventing and treating tetranychus urticae, and / or preparing a medicine for preventing and treating tetranychus urticae; and 2) reducing the resistance of the tetranychus urticae to the acaricide, and / or preparing a drug for reducing the resistance of the tetranychus urticae to the acaricide. The gRNA is designed by applying a gene knockout technology, especially aiming at a detoxification enzyme gene for regulating detoxification metabolism in a pest mite body, a compound consisting of the gRNA composition and cas9 protein is delivered into a tetranychus urticae body by utilizing a microinjection technology, and the gRNA composition loses the function of a target gene, reduces the detoxification metabolism capability of a medicament, and can be used for treating the pest mite body. The resistance reversion of the two-spotted spider mites is promoted, so that the aim of preventing and treating the two-spotted spider mites is

The invention relates to aldehyde ketoreductase and application thereof in degradation of 3-keto-DON, and belongs to the technical field of bioengineering. The amino acid sequence of the aldoketoreductase is as shown in SEQ ID NO. 1. The aldoketoreductase can realize heterologous high-efficiency expression in a prokaryotic expression system, and is easy for later-stage purification of detoxification enzyme and preparation of enzyme preparations. The aldehyde ketoreductase disclosed by the invention can be used for effectively degrading the 3-keto-DON toxin, and the degradation rate reaches 90% or above. Compared with physical detoxification, biological detoxification is more environmentally friendly, secondary pollution is avoided, and the efficient 3-keto-DON detoxification capacity has great application potential in the field.

The application discloses a sorbitol dehydrogenase mutant capable of efficiently degrading vomitoxin without relying on exogenous mediators, and belongs to the field of agricultural biotechnology. The mutant is obtained by rational design and directional evolution of wild-type sorbitol dehydrogenase (SDH), and contains three mutant sites of F103A, S454E and T492E, and the amino acid sequence and the nucleotide sequence are respectively SEQ ID NO. 1 and SEQ ID NO. 2. F103A / S454E / T492E The mutant SDH can efficiently degrade vomitoxin (DON) without relying on electron transfer replacement phenazine methosulfate (PMS), and the degradation efficiency can reach 82% in the absence of PMS. The application can be applied to the preparation of vomitoxin detoxification enzyme, and has a good application prospect in the biological detoxification of DON in feed, grain and food.

The invention relates to quinone-dependent dehydrogenase and application thereof in degradation of deoxynivalenol, and belongs to the technical field of bioengineering. The amino acid sequence of the quinone-dependent dehydrogenase is as shown in SEQ ID NO. 1. The quinone-dependent dehydrogenase can realize heterologous high-efficiency expression in a prokaryotic expression system, is easy for later-stage purification of detoxification enzyme and preparation of enzyme preparations, has higher degradation efficiency on a substrate DON, and is low in commercialized production cost. In addition, the invention finds that quinone-dependent dehydrogenase has a dependency relationship with the concentration of a cofactor pyrroloquinoline quinone (PQQ) in the degradation and conversion process of deoxynivalenol.

The application isolates a strain of Slackia bacteria D-W1 from goose intestinal tract, which is named Slackia sp. D-W1. The average nucleotide identity analysis (ANI) of the strain and other strains in the Slackia genus is less than 80.5%, which belongs to a new species of the Slackia genus. The strain has been preserved in the Guangdong Microbial Culture Collection Center on March 9, 2023, and the preservation number is GDMCC NO: 63251. The strain can metabolize vomitoxin (Deoxynivalenol, DON) into almost non-toxic product DOM-1. The temperature for growth and metabolism of the strain is 32-42 DEG C, and the pH value is 3-10. The strain can be used for preparing DON detoxification metabolic enzyme, DON detoxification enzyme preparation and DON detoxification engineering bacteria, and is used for feed processing, food processing and livestock and poultry breeding.

The invention discloses bamboo shoot enzyme pulp based on bamboo shoot pulp enzymolysis and a preparation method thereof, and relates to the technical field of fermented food, the preparation method comprises the following steps: 1) bamboo shoot pretreatment and waste heat enzymolysis; 2) in-situ detoxification and fermentation; (3) filtering and seasoning; and 4) aseptic packaging and storage. Enzymolysis, directional detoxification and segmented fermentation are integrated, detoxification enzyme and a compound enzyme preparation are synchronously added, and the reaction is started by utilizing fixation waste heat, so that the technological process is shortened by 40% or above, and the energy consumption is remarkably reduced; through an in-situ detoxification fermentation mechanism, toxins such as tannin, oxalic acid and cyanoglycoside are synchronously degraded in a microbial metabolism process, and the safety and the efficiency are synchronously improved; by adopting the graded filtering and flexible blending technology, low-sugar and sugar-free products can be accurately produced on the same production line, the limitation of single product in the traditional process is broken through, and the unification of high efficiency, safety and multiple market demands is realized.

The application belongs to the technical field of protein engineering, and particularly relates to a high-thermal-stability vomitoxin detoxification fusion enzyme mutant and application thereof. With an artificially constructed vomitoxin detoxification fusion enzyme ADDE as a parent, a protein language model SaProt is combined with three-dimensional structure analysis to identify that a proline at a 103th position of the vomitoxin detoxification enzyme is a key regulation site of thermal stability, and the proline is mutated into histidine to obtain a mutant P103H. The half-life of the mutant at 50 DEG C reaches more than 5 times of that of a wild type, while a high catalytic activity is maintained, and the mutant has a good application prospect in high-temperature environments such as feed processing and food industry.