41 results about "Detoxification enzymes" patented technology

The invetnio discloses hyper-engineering bacteria to decompose residual pesticide with reserving number at CGMCC No.1529, which is characterized by the following: cloning the gene of P450 CYP9G2 and P450 reductase on the pETDuet-1; constructing pETDuet-CYP-POR; transmitting into escherichia coli; sieving to obtain the product. The invention also provides detoxication enzyme, which is fit for detoxicating organophosphorus intoxication animal.

A method for identifying modulators of the Keap1-NrG-ARE pathway is described. In particular, an assay is described that identifies molecules that inhibit the binding of a labeled Nrf2 peptide with the kelch domain of the Keap1 protein. Molecules that inhibit the binding are activators of the Keap 1-Nrf2-ARE pathway. Activation of the Keap 1-Nrf2-ARE pathway may result in an increased accumulation of Nrf2 and the subsequent induction of protective enzymes, for example, the phase 2 detoxification enzymes. Activators of the Keap1-NrG-ARE pathway are useful for combating oxidative stress-related disorders, such as those associated with cancer, emphysema, Huntington's disease, light-induced retinal damage, and stroke.

The invention discloses an ochratoxin detoxification enzyme, an encoding gene, a recombinant vector and an application thereof, and belongs to the technical field of enzyme engineering. The inventionprovides the ochratoxin detoxification enzyme and the encoding gene thereof, and also provides the optimum pH of the ochratoxin detoxification enzyme and the application of the ochratoxin detoxification enzyme in degrading ochratoxin and ochratoxin biological detoxification in food. When ochratoxin A is processed by the ochratoxin detoxification enzyme at a temperature of 37 DEG C and at the pH of7.3 for 2min in a buffer system, the degradation rate of ochratoxin A reaches 100%, and the degradation efficiency is unprecedented in the field.

The pesticide degrading agent of esters specially for vegetable and fruit includes cell powder, anionic surfactant, citric acid, sodium bicarbonate and CMCNa. The cell powder is prepared with the microbe strain of the preservation number of CGMCC No. 0290 and through high density culture with detoxicated engineering bacteria to produce detoxification enzyme, fermentation, centrifugation, freeze drying and crushing. The pesticide degrading agent of the present invention can degrade the residual organic phosphate and pyrethrin pesticides in vegetable and fruit with relatively high effect.

The invention belongs to the field of toxicological activity of pest prevention and control, and particularly relates to a new drug for preventing and controlling Spodoptera litura, and a use method thereof, wherein the new drug is cyclosporin A, and the Spodoptera litura refers to second-instar and third-instar Spodoptera litura larvae. According to the invention, with the application of the newdrug in prevention and control of Spodoptera litura, the lethal concentration of the cyclosporin eaten by Spodoptera litura larvae is 100-150 [mu]M; the test results show that the biological source agent has good insecticidal activity on agricultural pests Spodoptera litura; cyclosporin A acts on Spodoptera litura, so that the activity of dephosphorylase is possibly inhibited so as to affect the expression and the activity of the antibacterial peptide and the detoxification enzyme in insects; and cyclosporin A or the compound with the similar chemical structure can possibly be the novel insecticide or can be used as a novel insecticidal functional group to be applied to development and utilization of novel pesticides.

According to the invention, the amino acid sequence of a zearalenone detoxification enzyme is optimized, and the 10 amino acid residues (10 amino acid differences exists with zhd 101 detoxification enzyme) of the detoxification enzyme are changed, so that the optimum reaction pH of the reassembled zearalenone detoxification enzyme is reduced from 8.5 to 6.5, and is very consistent with the pH of the intestinal tract of chicken and pigs; and when the pH is as low as 3.0, the detoxification enzyme activity remains 40% or above, so that the detoxification efficiency of the zearalenone detoxification enzyme as a feed additive in practical application is greatly improved. The invention also performs codon optimization on the zearalenone detoxification enzyme gene and expresses the gene in bacillus subtilis.

The present invention relates to a pharmaceutical composition preventing cancer and treating cancer and inflammation, which is characterized in that including xanthorrhizol as an active principle. Xanthorrhizol not only inhibits mutagenesis and tumor formation, and enhances the activity of detoxification enzyme of carcinogen, induces apoptosis of cancer cell, and suppresses the activity of COX-2 and iNOS which are related to tumor promotion and inflammatory reaction. Thus, a pharmaceutical composition including xanthorrhizol can be utilized for prevention of cancer and treatment of cancer and inflammation.

Compositions, systems, and methods for enhancing the ability of a subject to heal itself following an infection include administering a composition that includes transfer factor to a subject. Administration of such a composition or combination of compositions to a subject may result in improving the subject's overall antioxidant profile, increasing the concentration of chemical antioxidants present in the subject, increasing the efficiency with which the treated subject's enzymatic antioxidants work, increasing the efficiency and / or activity of the treated subject's detoxification enzymes, and improving cellular and molecular health of the subject.

The invention discloses a preparation method of a fruit and vegetable agriculture remanet removing agent. The preparation method comprises the following steps: 1) weighing small molecular water electrolysis, putting into a stirrer, and starting the stirrer; 2) adding KOH, uniformly stirring, and cooling to room temperature; 3) adding KCO3, and uniformly stirring; and 4) adding detoxification enzyme, and uniformly stirring, wherein the fruit and vegetable agriculture remanet removing agent is prepared from the following raw materials in percentage by weight: 5 percent of detoxification enzyme, 0.3-1 percent of KOH, 1 percent of KCO3 and 93-93.7 percent of small molecular water electrolysis. According to the preparation method disclosed by the invention, the small molecular water electrolysis is a natural nonionic surfactant, is colorless and tasteless, does not have foams and contain any surfactant, promoter and other harmful chemical substances, so that the worry of people that residues are generated as a cleaning product contains chemical promoters is exempted, and the fruit and vegetable agriculture remanet removing agent has the functions of strong decontamination ability, high bactericidal efficiency, removing peculiar smell, agriculture remanet and formaldehyde and the like.

The invention features a liver cell culture comprising hepatocytes that have increased detoxification enzyme activity when isolated from a liver of a donor that had been administered at least one induction agent prior isolation of hepatocyte cells. The induced hepatocytes are used in a bioreactor and cultured to produce hepatocyte cell products or metabolize toxins added to the culture. The bioreactor is, or is an integral part of, a liver assist device used to treat a patient in need of liver assist.

The invention discloses a sterilization treatment agent for wheat seeds. The sterilization treatment agent is prepared from the following raw materials in parts by weight: 10-20 parts of nonionic polyacrylamide, 1-2 parts of methyl isothiocyano formate, 5-15 parts of an organic silicon modified starch aqueous emulsion, 6-14 parts of grafted modified soybean protein, 1.5-6.5 parts of an alterneriatenuissima protein, 1-5 parts of eugenol, 5-10 parts of a bacterial enzyme mixture, 5-10 parts of a carbon enzyme, 7-15 parts of sodium para-nitrophenolate and 10-20 parts of ethyl formate. The bacterial enzyme mixture is prepared by mixing bacillus cereus, bacillus licheniformis and beta-composite detoxification enzyme according to a mass ratio of 5:2:3. The sterilization treatment agent providedby the invention has the advantages of high efficiency, low toxicity, low residue, good fast-acting property, long lasting period, environment friendliness and no easiness of generating drug resistance and the like, and the germination rate of the seeds can be improved.

The invention discloses an application of a biphenyl compound in preparing medicines for treating oxidative stress induced diseases. The chemical formula of the biphenyl compound is shown as Formula (as shown in the Description), wherein R1 is -H, -CH3, Formula (as shown in the Description) or Formula (as shown in the Description); R2 is -H, -CH3, Formula (as shown in the Description) or Formula (as shown in the Description); X1 is -H, Na<+>, K<+> or NH4<+>; X2 is -H, Na<+>, K<+> or NH4<+>; and X3 is -H, Na<+>, K<+> or NH4<+>. With the application of the biphenyl compound, the protein levels of Nrf2 and antioxidase [gamma]GCS and II-phase detoxification enzyme NQO1 regulated and controlled by the Nrf2 can be up-regulated; the GSH level of a cell endogenous antioxidant can be improved, and arsenic-induced Beas-2B cell injury and apoptosis can be inhibited; and a similar cytoprotection effect can be achieved on glomerular mesangial cells SV40MES13, human nerve SHSY5Y cells and human breast carcinoma MDA-MB-231 cells.

The invention belongs to the field of medicines, and relates to a separating method of isoflavone derivatives and application thereof. The invention discloses application of isoflavone in preparationof medicines for treating oxidative stress-induced diseases. A chemical formula of the isoflavone is shown in the attached figure. The isoflavone can be used for regulating up the protein levels of Nrf2 (nuclear factor-erythroid 2-related factor 2) and Nrf2-regulated II phase detoxification enzyme NQO1 and anti-oxidizing enzyme GCS; an activation mechanism is achieved by increasing the protein stability of Nrf2 to inhibit the protein degrading of Nrf2; an arsenic-induced lung bronchial epithelium Beas-2B cell injury model is used for evaluating the cell protecting function of the isoflavone; proofed by results, the isoflavone can increase the GSH level of cell endogenous antioxidant, and the injury and withering of arsenic-induced Beas-2B cell are inhibited.

The invention discloses a culture medium for multiplying test-tube seedlings based on somatic embryos of a pear tree. The culture medium is prepared from the following raw materials in parts by weight: 14-26 parts of an organosilicone modified starch water emulsion, 10-20 parts of 1 / 2 modified SH, 20-30 parts of sucrose, 20-30 parts of non-ionic polyacrylamide, 5-11 parts of graft modified soybeanprotein, 15-25 parts of alternaria tenuissima protein, 11-19 parts of carbonase, 11-19 parts of sodium p-nitrophenolate, 6-8.4 parts of eugenol, 1.5-6.5 parts of paclobutrazol, 1-3 parts of bacilluscereus, 3-6 parts of a beta-compound detoxification enzyme, 3-5 parts of bacillus natto, 3-5 parts of 6-benzyl aminopurine, 0.5-1.0 part of naphthylacetic acid and 3-6 parts of 2, 4-D. Through optimization of the components of the culture medium, the survival rate of transplanted plants is greatly increased, the incidence of diseases of the seedlings is reduced and the seedlings grow healthily andstrongly.

The invention belongs to an application of an insect detoxification enzyme in pesticide degradation, in particular relates to an application of a detoxification enzyme LmGSTpcF1 in locusta migratoriafor degrading malathion pesticide, The detoxification enzyme LmGSTpcF1 in locusta migratoria has amino acid sequence as shown in SEQ ID NO: 2. After incubating LmGSTpcF1 and GSH with malathion under reaction conditions, the malathion is detected by liquid chromatography and the result show that the malathion is completely degraded substantially and no pesticide residues is detected and the degradation efficiency is more than 99%. The detoxification enzyme LmGSTpcF1 in locusta migratoria can be used to remove malathion pesticide residues in agricultural products, water sources, soils, etc.

The invention discloses applications of pinoresinol in preparing products for preventing and treating diseases induced by oxidative stress. The chemical formula of pinoresinol is shown in the description, for the pinoresinol, by activating the Nrf2 signal channel, the protein levels of the antioxidant enzyme gamma GCS and the II phase detoxification enzyme NQO1 can be up-regulated, the GSH level of the endogenous antioxidant of cells is increased, and the cellular injuries induced by oxidative stress are inhibited; the pinoresinol has the similar cell protection effects for the human bronchusepithelial Beas-2B cells, the human renal glomerular endothelial cells HRGEC cells, the human nerve SHSY5Y cells and the human breast cancer MDA-MB-231 cells. The lignans can be used for preparing health products and medicines for preventing or treating diseases induced by oxidative stress.

The present invention discloses an enzyme natural therapy for conditioning high blood pressure, diabetes and gout related disorders. The enzyme natural therapy is characterized by containing the following steps: (1) step of hepatobiliary detoxification: compound detoxification enzymes are used to conduct hepatobiliary and intestinal detoxifications and thoroughly clean up the body internal environment, and the detoxification process lasts for two days; (2) step of intensified conditioning: enzyme meal replacement step is conducted after the completion of the hepatobiliary detoxification, and compound bone strengthening mate enzymes are used to replace meals for 14 to 21 days; at the same time three-vine foot soaking soup is used to soak feet once a night continuously for 30 days; meanwhile, tea drink is replaced by black tea made from haws, Chinese wolfberry fruits and red dates, which is drunk for 30 days; (3) step of rehabilitation nourishing: semi-meal replace step is conducted after the completion of the step (2), the compound bone strengthening mate enzymes are used to replace meals in the morning and in the evening, and porridge made from millet, rice and glutinous rice are eaten at noon; and each meal at noon is eaten to be moderate full, and this process continues for a total of 10-20 days; and (4) step of post-consolidation: after the step (3) is completed, the compound body and bone strengthening mate enzymes are eaten once every morning and evening or every morning and afternoon, three meals a day are normally taken, and at the same time a light diet of being moderate full is maintained, and this process is completed in a total 30 days.

The invention belongs to the technical field of feed additives, and concretely relates to a detoxifying enzyme of aflatoxin in a feed. The aflatoxin detoxifying enzyme comprises cutin protease, neutral protease, polyphenol oxidase, hemicellulase, glucose oxidase, lipase, glycosidase, a Rhizoma Polygoni Cuspidati extract, mung bean powder, vitamin A and vitamin D. The detoxifying enzyme of aflatoxin in the feed can decompose aflatoxin contained in the feed, also can hydrolyze macromolecular substances in the feed, and provides micro-molecular nutritional substances which can be digested and utilized for animals.

The invention belongs to the field of agricultural biotechnology, and in particular relates to fumonisin degrading enzyme FumDSB and its gene and application. The invention provides a fumonisin-degrading enzyme FumDSB from Sphingomonadales Bacterium, the amino acid sequence of which is shown in SEQ ID NO.1, and the invention provides a coding gene encoding the above-mentioned detoxification enzyme. The fumonisin degrading enzyme of the invention can be applied to industries such as agriculture, feed and food, and reduces the harm of fumonisin to animal and human health.

The invention discloses a Chinese herbal medicine formula with a detoxifying effect as well as a preparation method and application thereof. The Chinese herbal medicine formula with the detoxifying effect consists of the following raw materials in percentage by weight: 60-80% of pennisetum purpureum, 1-5% of dandelion, 1-5% of paris polyphylla, 1-5% of oldenlandia diffusa, 3-8% of astragalus membranaceus, 3-8% of schisandra chinensis, 1-5% of glossy privet fruit, 1-5% of liquorice, 1-5% of coptis chinensis, 1-5% of golden cypress and 1-5% of scutellaria baicalensis. The invention has the beneficial effects that the formula is formed by natural pennisetum purpureum containing a large amount of detoxification enzymes and various Chinese herbal medicines, can be used for treating diseases such as diabetes mellitus, uremia, rheumatism, gout, nephropathy, hyperlipidemia, osteoporosis, diuresis and the like, and can achieve the effects of removing harmful chemical medicines in a body, expelling toxins and repairing damaged cells. The Chinese herbal medicine formula not only can help patients to recover and strengthen body immunity, but also can help sub-healthy people and healthy people to resist diseases.

The pesticide degrading agent of esters specially for vegetable and fruit includes cell powder, anionic surfactant, citric acid, sodium bicarbonate and CMCNa. The cell powder is prepared with the microbe strain of the preservation number of CGMCC No. 0290 and through high density culture with detoxicated engineering bacteria to produce detoxification enzyme, fermentation, centrifugation, freeze drying and crushing. The pesticide degrading agent of the present invention can degrade the residual organic phosphate and pyrethrin pesticides in vegetable and fruit with relatively high effect.

The invention discloses a high-efficiency degradation of aflatoxin B 1 Escherichia coli ( Escherichia coli ) strain CG1061. The strain was deposited in the Guangdong Microbial Culture Collection Center on February 1, 2018, with the preservation number GDMCC NO: 60324. The strain is isolated from chicken cecum and is a non-pathogenic Escherichia coli that can efficiently degrade AFB 1 , can be applied to the preparation of aflatoxin B 1 Detoxification bacteria agents, detoxification enzyme preparations, etc., have good application prospects in food, feed processing and other fields.

The invention features a liver cell culture comprising hepatocytes that have increased detoxification enzyme activity when isolated from a liver of a donor that had been administered at least one induction agent prior isolation of hepatocyte cells. The induced hepatocytes are used in a bioreactor and cultured to produce hepatocyte cell products or metabolize toxins added to the culture. The bioreactor is, or is an integral part of, a liver assist device used to treat a patient in need of liver assist.

The invention discloses an aflatoxin detoxification enzyme with improved resistance to trypsin. The invention transforms the key amino acid residues in the outer part of the three-dimensional structure of the wild-type aflatoxin detoxifying enzyme through protein engineering technology, and provides an ADTZ mutant with improved resistance to trypsin. The mutant aflatoxin detoxifying enzyme (ADTZK221C / K252Q / K278S) acting on aflatoxin described in the present invention has 2.73 times higher resistance to trypsin than wild-type ADTZ, and its half-life is 72 minutes longer than the wild-type enzyme , and other enzymatic properties are basically the same as the wild-type enzyme.

The invention discloses a method of using amiRNAs to obtain safe and selective killing of transgenic rice: while expressing exogenous genes, endogenous miRNAs are used as precursors to selectively inhibit the expression of herbicide detoxification enzymes in rice, thereby obtaining safe, Genetically modified rice with optional kill. Specifically: the exogenous gene expression frame and the amiRNAs expression frame for directional inhibition of detoxification enzymes are constructed on the same plant expression vector; The sub-functionality is connected. The invention obtains a method for safely selecting and eradicating transgenic rice by directional silencing the expression of enzyme genes involved in herbicide detoxification and detoxification metabolism in rice.