Streptomyces canus and its application in degrading chloronicotinyl insecticide

A technique for neonicotinoid insecticides and Streptomyces griseus, which is applied in the field of Streptomyces fumigatus and its application in the degradation of neonicotinoid insecticides, achieving good application prospects

Inactive Publication Date: 2017-07-21
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, some microorganisms such as Ensifer melilotiCGMCC7333, Variovorax boronicumulans CGMCC 4969, Pigmentiphaga sp.AAP-1 and D-2, Rhodotorulamucilaginosa IM-2, maltophilia Stenotrophomonas sp.THZ-XP and Stenotrophomonas maltophilia CGMCC 1.1788 and Rhodococcus sp.BCH2 have been reported to degrade acetamiprid or thiacloprid, but there is no research on the degradation of acetamiprid and thiacloprid by actinomycetes and patent coverage

Method used

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  • Streptomyces canus and its application in degrading chloronicotinyl insecticide
  • Streptomyces canus and its application in degrading chloronicotinyl insecticide
  • Streptomyces canus and its application in degrading chloronicotinyl insecticide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Isolation, Screening, Identification and Biological Characteristics of Biodegradable Acetamiprid Strain

[0021] 1. Bacteria isolation

[0022] Soil was collected from Xuzhou, Jiangsu, and 2 g of soil was added to 18 mL of sterile water containing glass beads, shaken for 2 hours, and after standing for 20 minutes, 100 μL of the sample was diluted to 10 -3 and 10 -4 After that, it was spread on a solid plate of mineral salt medium containing 50 mg / L acetamiprid. The composition of the mineral salt medium is (g / L): KH 2 PO 4 1.36, Na 2 HPO 4 2.13, MgSO 4 ·7H 2 O 0.5 and 10 mL metal ionic liquid, pH 7.0. The composition of metal ionic liquid is (g / L): CaCl 2 ·2H 2 O0.40,H 3 BO 3 0.30, CuSO 4 ·5H 2 O0.04, KI0.10, FeSO 4 ·7H 2 O0.20, MnSO 4 ·7H 2 O0.40, NaMoO 4 ·2H 2 0.20 and 10.0mL / L concentrated hydrochloric acid. The samples were cultured for 7 days in a shaker at 30°C with a rotational speed of 220 rpm, and the culture solution was spread ...

Embodiment 2

[0028] Example 2: Degradation of acetamiprid by growth cells of Streptomyces griseus CGMCC 13662

[0029]The preserved Streptomyces cinerea CGMCC 13662 was streaked on Gao's No. 1 solid medium, and cultured in a 30°C incubator until spores grew (about 7 days). The spores were collected with sterile water and a spore suspension was prepared, inoculated into a 500 mL Erlenmeyer flask containing 100 mL of ISP4 liquid medium (containing 200 mg / L acetamiprid), and cultured with shaking at 30° C. and 220 rpm. After culturing for 4 days, sampling was carried out for HPLC analysis, and the results showed that the acetamiprid content of the experimental group inoculated with Streptomyces grisea CGMCC 13662 was 0.26mmol / L (as shown in the appendix). figure 2 Shown in A); the acetamiprid content of the control group without inoculation was 0.94mmol / L (as shown in the appendix). figure 2 shown in B). The results showed that 73% acetamiprid could be degraded by the growth cell of Strep...

Embodiment 3

[0030] Example 3: Resting Cell Degradation of Acetamiprid by Streptomyces cinerea CGMCC 13662

[0031] The preserved Streptomyces cinerea CGMCC 13662 was streaked on Gao's No. 1 solid medium, and cultured in a 30°C incubator until spores grew. The spores were collected with sterile water and a spore suspension was prepared, inoculated into a 100 mL Erlenmeyer flask containing 30 mL of ISP4 liquid medium, and cultured with shaking at 30°C and 220 rpm. When the cells grow into mycelial balls, weigh 0.3 of the wet cells and wash them twice with 0.2 mol / L phosphate buffer (pH 7.5), then suspend them in 15 mL of the above-mentioned phosphate buffer (containing 200 mg / L of phosphate buffer). Acetamiprid) in a 50 mL centrifuge tube, then transferred to a 100 mL conical flask, and cultured at 30°C with shaking at 220 rpm for 96 h. HPLC analysis of the degradation of acetamiprid, as attached image 3 As shown, the results showed that acetamiprid was reduced by 0.47 mmol / L, and the de...

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Abstract

The invention discloses streptomyces canus; through identification, it is streptomyces canus, and the bacterial strain name is gl2-18; the streptomyces canus is preserved at China General Microbiological Culture Collection Center; the preservation number is CGMCC No. 13662; the preservation date is February 15, 2017. Compared with the prior art, the resting cell or the growth cell of the new strain streptomyces canus CGMCC 13662 can effectively degrade chloronicotinyl insecticide, in particular to acetamiprid and thiacloprid; the streptomyces canus is used as the pesticide degradation fungicide, and has very good application prospect.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Streptomyces cinerea and its application in degrading nicotinic pesticides. Background technique [0002] Nicotinic insecticides are insecticides that act on nicotinic acetylcholine receptors at synapses in the insect nervous system. Acetamiprid is a nitromethylene heterocyclic insecticide that acts on nicotinic acetylcholine receptors at synapses in the insect nervous system. Acetamiprid is highly effective and broad-spectrum on piercing and sucking mouthpart pests, and is widely used in the control of aphids, planthoppers, thrips and some lepidopteran pests of rice, vegetables, fruit trees and tea. However, the long-term and large-scale use of acetamiprid has had a serious impact on food safety and ecological environment, such as ecological chain problems caused by high toxicity to pollinators, bees and birds; food safety caused by residues in grains, vege...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02A62D101/04C12R1/465
CPCA62D3/02A62D2101/04C12N1/20C12N1/205C12R2001/465
Inventor 戴亦军方文婉郭铃陈晨周伊玲芮雪
Owner NANJING NORMAL UNIVERSITY
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