Toxoplasma gondii rapid detection kit and preparation method thereof

A detection kit, a technology for Toxoplasma gondii, which is applied in the field of temperature amplification, can solve the problems of affecting specificity and sensitivity, and long reaction time, and achieves the effects of rapid reaction, simple operation, and long detection time.
CN101974634AActive Publication Date: 2011-02-16LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Publication Date
2011-02-16

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Abstract

The invention relates to a Toxoplasma gondii rapid detection kit and a preparation method thereof. The kit is based on the molecular biology and the nucleoside triphosphate hydrolase (NTPase) gene with high conservation of Toxoplasma gondii to adopt the loop-mediated isothermal amplification (LAMP) method to perform the rapid detection of Toxoplasma gondii. The kit contains LAMP reaction solution, primer, Bst DNA polymerase, color reagent, standard positive template and dd H2O. The kit of the invention is characterized by high sensitivity and specificity, rapid reaction, simple operation and the like, does not require special instrument and equipment and solves the defects of the prior art such as long detection time of toxoplasmosis, large workload and complicated operation. The kit of the invention can be used to detect whether a clinical sample contains Toxoplasma gondii and used in the early diagnosis and the molecular epidemiological study of toxoplasmosis.
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Description

technical field

[0001] The invention relates to a circle-mediated isothermal amplification technology for detecting toxoplasma genome DNA, specifically a toxoplasma rapid detection kit, and the invention includes a preparation method of the kit. Background technique

[0002] The commonly used methods for diagnosing toxoplasmosis include agglutination test, enzyme-linked immunosorbent assay and PCR, etc., and these methods have certain shortcomings. The agglutination test is mainly the indirect hemagglutination method. The disadvantage of this method is that the detection sensitivity is low, and there may be cases of missed detection; the disadvantage of the enzyme-linked immunosorbent assay is that the required equipment is expensive, and it needs to be used in conjunction with a special microplate reader and operation. The process is relatively complicated and the detection time is relatively long; moreover, these two methods are for the detection of Toxoplasma gondii antib...

Claims

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