GTP cyclohydrolase I gene folE and application

Abstract

The invention belongs to the field of microbial genetic engineering, and discloses a GTP cyclohydrolase I gene folE and application. A nucleotide sequence of the GTP cyclohydrolase I gene folE is shown as SEQ ID NO:1. The gene is derived from food-borne lactobacillus plantarum, is safe and can be used in the field of later-stage food fermentation. A gene knockout strain screening method can be used for improving the screening efficiency of knockout strains. The key role of gene folE gene in folic acid synthesis is proved, and a certain theoretical basis is provided for the research and development of folic acid synthesis functional food. The GTP cyclohydrolase I gene folE and application analyze that the gene is derived from food-grade microorganisms and is safe; and the GTP cyclohydrolaseI gene folE and application analyze that the gene folE gene has nothing to do with cell morphology and strain growth except playing an important role in folic acid synthesis.

Description

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AI Technical Summary

Benefits of technology

This patented technology describes two benefits - firstly, its use comes naturally because they were found on vegetables grown by farmers or other crops where there was no known harmful bacteria like Escherichia coli (BGE) inside them during production. Secondly, this new discovery allows us to study how certain specific factors affect the activity of these enzyme molecules involved in making hormones called gamma lactone. These findings help scientists better identify potential sources of health hazards associated with eaten raw materials such as BGA.

Problems solved by technology

This patented problem addressed in this patents relates to improving the efficiency at producing follicular acid without generating excessively harmful substances like nitrogen oxide gamma ray emitters. Previous attempts were made either by adding antimicrobium salts alone or combining them together into mixtures containing both folic acids and folacycliridones. These approaches resulted in reduced yields when folic Acid had low solubility in aqueous solution. To overcome these issues, we developed new techniques involving modifying specific proteins within cells to enhance their activities and reduce unwanted side actions associated with folic ester degradation systems.

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