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Solid fermentation method for producing salt-tolerant cellulase by using oceanic Aspergillus niger

A marine Aspergillus niger and cellulase technology, applied in microorganism-based methods, biochemical equipment and methods, enzymes, etc., can solve problems such as low utilization efficiency and utilization limitations, and achieve high equipment utilization, simple cultivation process, Effects that are easy to control and magnify

Inactive Publication Date: 2011-04-13
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Vegetation growing in saline-alkali land is rich in high salinity, its effective and reasonable utilization is greatly limited, and the utilization efficiency is low

Method used

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  • Solid fermentation method for producing salt-tolerant cellulase by using oceanic Aspergillus niger
  • Solid fermentation method for producing salt-tolerant cellulase by using oceanic Aspergillus niger
  • Solid fermentation method for producing salt-tolerant cellulase by using oceanic Aspergillus niger

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1) A marine Aspergillus niger was screened from the soil at a depth of 10m off the coast of the East China Sea;

[0036] 2) Marine Aspergillus niger was inoculated on PDA medium, cultured at 40°C for 90 hours to obtain slant spores, which were stored at 4°C;

[0037] 3) The slant spores were inoculated in PDA-1 medium, and cultured at 40° C. for 90 hours to obtain activated spores;

[0038] 4) Put 60g of eggplant bottle slant culture medium into a 250ml eggplant bottle and compact it into a slant, seal the mouth of the bottle with 8 layers of gauze to obtain the slant of the eggplant bottle, inoculate the activated spores on the slant of the eggplant bottle, culture at 42°C for 74 hours, and obtain the expanded culture eggplant bottle slant spores;

[0039] 5) Put 400ml of artificial seawater into a 500ml volumetric flask, put it into a sterilizer, and sterilize it at 121°C for 20 minutes to obtain sterilized artificial seawater, and dilute the slanted spores of the eg...

Embodiment 2

[0047] 1) A marine Aspergillus niger was screened from the soil at a depth of 10m off the coast of the East China Sea;

[0048] 2) Marine Aspergillus niger was inoculated on PDA medium, cultured at 40°C for 90 hours to obtain slant spores, which were stored at 4°C;

[0049] 3) The slant spores were inoculated in PDA-1 medium, and cultured at 40° C. for 90 hours to obtain activated spores;

[0050] 4) Put 60g of eggplant bottle slant culture medium into a 250ml eggplant bottle and compact it into a slant, seal the mouth of the bottle with 8 layers of gauze to obtain the slant of the eggplant bottle, inoculate the activated spores on the slant of the eggplant bottle, culture at 42°C for 74 hours, and obtain the expanded culture eggplant bottle slant spores;

[0051] 5) Put 400ml of artificial seawater into a 500ml volumetric flask, put it into a sterilizer, and sterilize it at 121°C for 20 minutes to obtain sterilized artificial seawater, and dilute the slanted spores of the eg...

Embodiment 3

[0057] 1) A marine Aspergillus niger was screened from the soil at a depth of 10m off the coast of the East China Sea;

[0058] 2) Marine Aspergillus niger was inoculated on PDA medium, cultured at 40°C for 90 hours to obtain slant spores, which were stored at 4°C;

[0059] 3) The slant spores were inoculated in PDA-1 medium, and cultured at 40° C. for 90 hours to obtain activated spores;

[0060] 4) Put 60g of eggplant bottle slant culture medium into a 250ml eggplant bottle and compact it into a slant, seal the mouth of the bottle with 8 layers of gauze to obtain the slant of the eggplant bottle, inoculate the activated spores on the slant of the eggplant bottle, culture at 42°C for 74 hours, and obtain the expanded culture eggplant bottle slant spores;

[0061] 5) Put 400ml of artificial seawater into a 500ml volumetric flask, put it into a sterilizer, and sterilize it at 121°C for 20 minutes to obtain sterilized artificial seawater, and dilute the slanted spores of the eg...

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Abstract

The invention discloses a solid fermentation method for producing salt-tolerant cellulase by using an oceanic Aspergillus niger, comprising the following steps of: (1) screening an oceanic Aspergillus niger from soil in the depth of 10-20m approaching to the East China Sea; (2) vaccinating the oceanic Aspergillus niger on a PDA culture medium for culturing to obtain an inclined-plane spore, and preserving the inclined-plane spore at 4 DEG C; (3) vaccinating the inclined-plane spore on a PDA-1 culture medium for culturing to obtain an activated spore; (4) vaccinating the activated spore on an eggplant bottle inclined plane for culturing for 74h at 42 DEG C to obtain an eggplant bottle inclined-plane spore; and (5) propagating the obtained eggplant bottle inclined-plane spore, diluting the eggplant bottle inclined-plane spore to 108 spores / ml by using sterilized artificial seawater, vaccinating to a solid fermentation enzyme-producing culture medium, and fermenting for 8-12 days to obtain the cellulase. The solid fermentation method has the characteristics of coarse culture medium, wide source, low price and the like, the produced cellulase can degrade cellulose under the condition of a higher salt content, and the enzyme is abnormally stable under high-salt environment, therefore, the produced cellulase lays the foundation for the subsequent application of hydrolysate.

Description

technical field [0001] The invention relates to a solid fermentation method for producing salt-tolerant cellulase by marine Aspergillus niger. Background technique [0002] Cellulosic materials hold great promise for the production of biobased products. There are a large number of cellulose material resources in nature, such as corn straw, rice straw, wheat straw, rape straw and so on. The rich cellulose contained in these substances can be degraded to obtain fermentable sugar-glucose, which can be used to produce a large number of bio-based products, such as bioethanol, butanol, citric acid, lactic acid, single-cell protein, etc. In particular, the use of cellulosic materials to produce bioenergy such as hydrogen and ethanol can alleviate the increasingly tense energy pressure, and has very broad prospects. [0003] A key step in the utilization of cellulosic material is its conversion into fermentable sugars. There are usually two types of methods to degrade cellulose: ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12N1/14C12R1/685
Inventor 姚善泾薛栋升林东强刘杰凤
Owner ZHEJIANG UNIV
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