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Preparation method of magnetic-cellulose-microsphere-immobilized lipase catalyst

A technology for immobilizing lipase and cellulose microspheres, which can be used in biochemical equipment and methods, immobilized on/in organic carriers, enzymes, etc., and can solve the problem of restricting the large-scale application of lipase, difficult to recover, separate and purify , lipase inactivation and other problems, to achieve the effect of being conducive to recycling and reuse, low price and high selectivity

Active Publication Date: 2017-11-07
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, lipase is easily inactivated by the environment, has poor stability, and is difficult to recover and separate and purify. These shortcomings limit the large-scale application of lipase in industrial production.

Method used

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  • Preparation method of magnetic-cellulose-microsphere-immobilized lipase catalyst
  • Preparation method of magnetic-cellulose-microsphere-immobilized lipase catalyst

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Preparation of regenerated cellulose microspheres by hot melt adhesive conversion method: Soak absorbent cotton with a certain amount of lye for 2-5 hours, then drain the solution, leave it for aging for 40-50 hours, add appropriate amount of CS 2 Sulfonate at 25-40°C for 6-10 hours, add appropriate amount of lye and CaCO 3 Stir evenly, pour into 400~600mL transformer oil containing 0.05~0.1g potassium oleate, 0.2~0.4g Span 60, stir at room temperature for 0.5~2h, then slowly raise the temperature to 70~90℃, and cure for 1.5~3.5h, Pour out the oil, wash the solid with a large amount of hot water until there is no oil, and obtain cellulose microspheres; add the cellulose microspheres to an appropriate amount of dilute hydrochloric acid solution, shake until there are no bubbles, pour out the liquid, add new dilute hydrochloric acid, and repeat several times , until the liquid becomes acidic, and the porous cellulose microspheres are prepared.

Embodiment 2

[0021] Chemical modification: add porous cellulose microspheres to epichlorohydrin, add an appropriate amount of lye to react, and prepare epoxidized cellulose microspheres; add appropriate amount of epoxidized cellulose balls to 8-12mL In the amine modification reagent, react at 40-60° C. for 8-12 hours to obtain amino-modified cellulose microspheres.

Embodiment 3

[0023] Preparation of magnetic cellulose microspheres by co-precipitation: add amino-modified cellulose microspheres to 100-200mL Fe 3+ and Fe 2+ The mixed liquid is vacuum filtered at 0-25°C for 1 hour, ammonia water is added to adjust the pH of the liquid to 9-11, and coprecipitation is carried out at 50-60°C for 1-3 hours to prepare the magnetic cellulose microsphere matrix.

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Abstract

The invention relates to a preparation method of a magnetic-cellulose-microsphere-immobilized lipase catalyst. The object is to provide a simple, economic, and efficient preparation method of an immobilized lipase catalyst. The adopted process and method comprise: taking cellulose as a raw material, taking solid calcium carbonate particles as a pore-forming agent, through adoption of a hot melt adhesive conversion method and combination with reverse suspension technology, preparing porous regenerated cellulose microspheres, carrying out epoxidation and amination grafting modification, taking micropores as a reactor for a co-precipitation reaction to prepare magnetic cellulose microspheres, and using a covalent bonding method to immobilize lipase molecules to surfaces and micropores of the magnetic cellulose microspheres. The method is abundant and available in raw materials, and the prepared magnetic cellulose carrier is relatively small in size, uniform in distribution, and large in specific surface area. Compared with a catalyst made from a conventional method, the catalyst is high in enzyme immobilization amount, good in dispersion, and firm in loading. The catalyst is convenient to recycle, and can be widely applied to fields of medicine, environment, and energy.

Description

technical field [0001] The invention relates to an immobilized lipase catalyst with magnetic cellulose microspheres as a carrier and a preparation method thereof, belonging to the technical field of immobilized enzyme biocatalyst preparation technology. Background technique [0002] Lipase, also known as triacylglycerol hydrolase, is an enzyme that catalyzes the hydrolysis of esters to produce fatty acids and glycerol. Lipase is a typical reversible enzyme, which can not only catalyze the hydrolysis of oil in the aqueous phase, but also catalyze the ester synthesis reaction in the non-aqueous phase, and these two reactions can be combined in a continuous manner to trigger a A series of reactions, such as exchange reaction, acidolysis reaction, alcoholysis reaction and ammonolysis reaction, etc. It can efficiently catalyze the reaction under mild conditions of normal temperature and pressure, and has specificity, and has more and more applications in medicine, environment, e...

Claims

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Application Information

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IPC IPC(8): C12N11/12
CPCC12N9/20C12N11/12C12Y301/01003
Inventor 付玉杰韩盈枝王希清焦骄罗猛刘志国王微
Owner NORTHEAST FORESTRY UNIVERSITY
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