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Hemoperfusion device filled with immobilized L-asparaginase and preparation method and application thereof

A technology of asparaginase and hemoperfusion, which is applied in the field of medical devices, can solve the problems of potential safety hazards, the enzyme liquid is easy to fall off and enter the human body, etc., and achieve the effect of good carrier strength, not easy to fall off and attenuate, and high catalytic efficiency

Active Publication Date: 2021-04-27
苏州森康微球医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If this invention is applied in hemoperfusion, there are still deficiencies. In actual clinical use, the adsorbed or embedded enzyme liquid is still easy to fall off and enter the human body, posing a safety hazard.

Method used

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  • Hemoperfusion device filled with immobilized L-asparaginase and preparation method and application thereof
  • Hemoperfusion device filled with immobilized L-asparaginase and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] (1) Preparation of amino carrier;

[0053] Polyvinyl alcohol and deionized water are used as dispersed phases; glycidyl methacrylate, divinylbenzene, and toluene are used as porogens, and BPO is used as an initiator; they are uniformly mixed to form an organic phase. The organic phase is added to the dispersed phase, and the suspension polymerization is carried out at a temperature of 90-95°C. After the polymerization reaction was completed, the porogen was rinsed with ethanol. Sieve out the appropriate particle size to obtain epoxy-based balls. The epoxy value of the epoxy base ball is 540μmol / g; the pore size is 90nm; the turbidity is 160 during the strength test; the particle size is 300μm; the water content is 85%. React epoxy-based spheres with ethylenediamine at a temperature of 90-100°C to obtain amine-based carriers.

[0054] (2) Pretreatment of amino-type carrier;

[0055] Put the amine-based carrier into the chromatography column, rinse from top to bottom ...

Embodiment 2

[0061] (1) Preparation of amino carrier;

[0062] Use dispersant and deionized water as dispersed phase; mix ethylene glycol dimethacrylate, allyl tripolyisocyanate, divinylbenzene, porogen isobutanol, and initiator BPO evenly to form an organic phase . The organic phase is added to the dispersed phase, and the suspension polymerization is carried out at a temperature of 60-70°C. After the polymerization reaction is completed, rinse the porogen with acetone. Sieve out the appropriate particle size to obtain epoxy-based balls. The epoxy value of the epoxy base ball is 480μmol / g; the pore size is 50nm; the turbidity is 190 during the strength test; the particle size is 300μm; the water content is 55%. React epoxy-based balls with butanediamine at a temperature of 80-90°C to obtain amine-based carriers.

[0063] (2) Pretreatment of amino-type carrier;

[0064] Put the amine-based carrier into the chromatography column, rinse from top to bottom with 4 times the volume of the ...

Embodiment 3

[0070] (1) Preparation of amino carrier;

[0071] Dispersant and deionized water are used as dispersed phase; glycidyl methacrylate, divinylbenzene, ethylene glycol dimethacrylate, allyl trimeric isocyanate, porogen methylcyclohexane, Initiator BPO, mixed evenly, is the organic phase. The organic phase is added to the dispersed phase, and the suspension polymerization is carried out at a temperature of 70-80°C. After the polymerization reaction is completed, rinse the porogen with acetone. Sieve out the appropriate particle size to obtain epoxy-based balls. The epoxy value of the epoxy base ball is 500μmol / g; the pore size is 70nm; the turbidity is 170 during the strength test; the particle size is 310μm; the water content is 75%. React epoxy-based spheres with hexamethylenediamine at a temperature of 60-70°C to obtain amine-based carriers.

[0072] (2) Pretreatment of amino-type carrier;

[0073] Put the amine-based carrier into the chromatography column, rinse from top ...

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Abstract

The invention discloses a hemoperfusion device filled with immobilized L-asparaginase and a preparation method and application thereof. The hemoperfusion device is filled with the immobilized L-asparaginase in the form of a packed column by adopting a covalent bond immobilization method and adopting a polymer carrier. The prepared hemoperfusion device filled with the immobilized L-asparaginase is safe and efficient in the aspect of treating acute lymphocytic leukemia.

Description

technical field [0001] The invention belongs to the field of medical devices, and in particular relates to an immobilized enzyme hemoperfusion device and its preparation method and application. Background technique [0002] L-asparaginase can hydrolyze L-asparagine (CAS: 70-47-3) into L-aspartic acid (CAS: 56-84-8), while avoiding asparagine through Maillard The reaction proceeds to brown to acrylamide. Acrylamide is a potential carcinogen in the body, it affects tumor suppressor genes, leading to accelerated formation of leukemia. Therefore, reducing the content of asparagine in the blood is an effective method for the treatment of acute lymphoblastic leukemia. French scientists first used L-asparaginase as a treatment for children with acute lymphoblastic leukemia. This enzyme can hydrolyze L-asparagine in serum to cause apoptosis and damage protein synthesis. However, this French drug will enter the enzyme into the human body. After that, there are serious side effects...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61M1/36C12N11/087
CPCA61M1/36C12N11/087C12N9/82C12Y305/01001
Inventor 林炳旺刘学
Owner 苏州森康微球医疗科技有限公司
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