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Wheat ration enzyme containing neutral protease and preparation method thereof

The technology of neutral protease and acid protease is applied in the field of wheat dietary enzyme and its preparation, which can solve the problems of complex structure and inability to measure, and achieve the effect of high temperature tolerance and wide applicable pH value range.

Active Publication Date: 2014-04-23
HUNAN HONGYING BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main reason is that the structure of the market is relatively complex, the market is diversified, and it is even impossible to measure

Method used

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  • Wheat ration enzyme containing neutral protease and preparation method thereof

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Effect test

Embodiment 1

[0057] A kind of wheat dietary enzyme containing neutral protease, is made up of the enzyme preparation of following parts by weight:

[0058] Bacillus subtilis culture 25 parts, acid protease 25 parts, acid xylanase 25 parts, cellulase 25 parts, β-glucanase 17 parts, amylase 17 parts, β-mannanase 12 parts, 12 parts of Chinese herbal medicine extract, 13 parts of protective agent, 12 parts of activator.

[0059] The acid protease, acid xylanase, cellulase, β-glucanase, amylase, and β-mannanase are all food-grade enzyme preparations;

[0060] The preparation method of described bacillus subtilis culture comprises the steps:

[0061] (1) Inoculate the well-preserved slant strain of Bacillus subtilis 1398-2-12 on the slant medium, culture at 30°C for 24 hours to activate the strain, and activate it twice;

[0062] The slant medium consists of: beef extract 3g, sodium chloride 5g, peptone 10g, glucose 2g, (NH) 2 SO 4 3g, K 2 HPO 4 6g, CaCl 2 1g, 15g agar, 5g Chinese herbal ...

Embodiment 2

[0096] A kind of wheat dietary enzyme containing neutral protease, is made up of the enzyme preparation of following parts by weight:

[0097] 20 parts of Bacillus subtilis culture, 20 parts of acid protease, 20 parts of acid xylanase, 20 parts of cellulase, 15 parts of β-glucanase, 15 parts of amylase, 10 parts of β-mannanase, 10 parts of Chinese herbal medicine extract, 10 parts of protective agent, 10 parts of activator.

[0098]The acid protease, acid xylanase, cellulase, β-glucanase, amylase, and β-mannanase are all food-grade enzyme preparations;

[0099] The preparation method of described bacillus subtilis culture comprises the steps:

[0100] (1) Activation of strains

[0101] The well-preserved slant strain of Bacillus subtilis 1398-2-12 was inoculated on the slant medium, cultured at 33°C for 30 hours to activate the strain, and activated twice in this way;

[0102] The slant medium consists of: beef extract 6g, sodium chloride 8g, peptone 15g, glucose 4g, (NH) ...

Embodiment 3

[0135] A kind of wheat dietary enzyme containing neutral protease, is made up of the enzyme preparation of following parts by weight:

[0136] 30 parts of Bacillus subtilis culture, 30 parts of acid protease, 30 parts of acid xylanase, 30 parts of cellulase, 20 parts of β-glucanase, 20 parts of amylase, 15 parts of β-mannanase, 15 parts of Chinese herbal medicine extract, 15 parts of protective agent, 15 parts of activator.

[0137] The acid protease, acid xylanase, cellulase, β-glucanase, amylase, and β-mannanase are all food-grade enzyme preparations;

[0138] The preparation method of described bacillus subtilis culture comprises the steps:

[0139] (1) Activation of strains

[0140] The well-preserved slant strain of Bacillus subtilis 1398-2-12 was inoculated on the slant medium, cultured at 36°C for 36 hours to activate the strain, and activated 3 times in this way;

[0141] The slant medium consists of: beef extract 10g, sodium chloride 12g, peptone 20g, glucose 5g, (...

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Abstract

The invention discloses wheat ration enzyme containing neutral protease and a preparation method thereof and belongs to the technical field of enzyme preparation. The wheat ration enzyme is formed by scientific compounding of bacillus subtilis culture, acid protease, acid xylanase, cellulose, beta-glucanase, amylase, beta-mannase, Chinese herb extract, protective agent and activating agent. Due to the bacillus subtilis culture, the immunity of organism of animals can be improved, the growth of the immune organ is enhanced, the maturity of the structure and the function of the intestine of the animal is enhanced, the daily gain of the animal is increased and the feed conversion ratio is improved. The wheat ration enzyme disclosed by the invention has the advantages that safe digestive enzyme is provided for livestock and poultry, the digestion burden is effectively reduced, the material utilization rate and the growth rate are increased, and the environment is protected; simultaneously, due to use of the proper amount of activating agent, the effect of the enzyme preparation can be fully played under the equal conditions, so that the adding amount of the enzyme preparation is saved; due to the scientific compounding of the Chinese herb extract, not only the quality guarantee period of the compound enzyme preparation is prolonged, but also the immunity of the raised livestock and poultry can be improved.

Description

technical field [0001] The invention belongs to the technical field of enzyme preparation, in particular to a wheat dietary enzyme containing neutral protease and a preparation method thereof. Background technique [0002] There are four main reasons for adding enzyme preparations to feed: 1. Degradation of anti-nutritional factors present in animal feed. These substances cannot be degraded by animal endogenous enzymes, thereby interfering with the normal metabolism of animals, resulting in indigestion of animals and decreased production performance. 2. Improve the utilization rate of starch, protein and minerals. These substances are either surrounded by fiber-rich cell walls, or are present in certain structures that cannot be digested by animals (for example, in plant feed materials, a large amount of phosphorus is present in the form of phytate phosphorus.). 3. Degrade some specific chemical bonds in raw materials. These chemical bonds cannot be degraded by the animal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/50C12N9/42C12N9/24A23K1/165A23K1/14
CPCA23K10/30A23K20/00A23K20/189C12N9/0006C12N9/0089C12N9/16C12N9/2402C12N9/2411C12N9/2425C12N9/2437C12N9/244C12N9/2445C12N9/2457C12N9/248C12N9/2491C12N9/50C12Y101/03004C12Y115/01001C12Y301/03002C12Y302/01002C12Y302/01021C12Y302/01025C12Y302/01041Y02A40/70
Inventor 李洪兵李海清张锦杰胡永明易继云
Owner HUNAN HONGYING BIOTECHNOLOGY CO LTD
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